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:: Volume 23, Issue 1 (4-2018) ::
__Armaghane Danesh__ 2018, 23(1): 14-28 Back to browse issues page
Study of the effect of wheat germ oilon carbon tetrachloride induced hepatic damage in adult male mice
Fatemeh Rezazadeh 1, Vahid Nejati 2, Ali Shalizar jalali 3, Gholamreza Najafi 3, Fatemeh Rahmani 2
1- MSc Student Urmia University , F.Rezazadeh000@Gmail.com
2- Associate Professor Urmia University
3- Assistant Professor Urmia University
Abstract:   (821 Views)
Background: Carbon tetrachloride, an organic solvent and chemical intermediate used in the industry, can cause numerous side effects including hepatotoxicity. This study was aimed to evaluate the possible hepatoprotective potential of wheat germ oil against carbon tetrachloride-induced hepatic disorders in male mice.
Materials and Methods: 48 male mice were randomly divided into 6 groups of 8 and treated for 4 weeks including control, carbon tetrachloride (1 ml/kg body weight intraperitoneally, 2 times a week),carbon tetrachloride (1 ml/kg body weight intraperitoneally) + wheat germ oil (250 mg/kg body weight per day orally), carbon tetrachloride (1 ml/kg) + wheat germ oil (500 mg/kg/day orally), wheat germ oil (250 mg/kg body weight per day orally) and wheat germ oil (500 mg/kg body weight per day orally) groups. At the end of the treatment, animal blood was collected to measure liver enzymes including AST, ALT and ALP. Hepatic tissue samples were harvested, fixed in 10% formalin, stained with hematoxylin and eosin and analyzed using optical microscopy. The data were analyzed by one-way ANOVA and Tukey's post hoc test.
Results: In mice receiving carbon tetrachloride combined with wheat germ oil (250 and 500 mg/kg), liver enzymes (ALP, ALT and AST) significantly (p<0.05) decreased compared to the carbon tetrachloride group. Lipid peroxidation in carbon tetrachloride + wheat germ oil (250 and 500 mg/kg) groupswas significantly (p<0.05) lower than the carbon tetrachloride group and levels of catalase and total antioxidant capacity significantly increased (p<0.05). Moreover, wheat germ oil at doses of 250 and 500 mg/kg improved tissue injuries due to administration of carbon tetrachloride.
Conclusion: These findings suggest that wheat germ oil can exert hepatoprotection in carbon tetrachloride evoked hepatotoxicities in mice. 
Keywords: Carbon tetrachloride, Wheat germ oil, Liver, Oxidative stress, Mice
Full-Text [PDF 1151 kb]   (99 Downloads)    
Type of Study: Research | Subject: Clinical
Received: 2017/09/3 | Accepted: 2018/01/21 | Published: 2018/02/26
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Rezazadeh F, Nejati V, Shalizar jalali A, Najafi G, Rahmani F. Study of the effect of wheat germ oilon carbon tetrachloride induced hepatic damage in adult male mice. Armaghane danesh. 2018; 23 (1) :14-28
URL: http://armaghanj.yums.ac.ir/article-1-1923-en.html


Volume 23, Issue 1 (4-2018) Back to browse issues page
ارمغان دانش Armaghane danesh
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