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The Healing effect of Teucriumpolium L. on Acetic Acid-Induced Colitis in Male Rat
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N Toneedeh1   , HA Lashkarpoor2 , S Daneshchi3 , SL Asadi Yousefabad 4, O Koohi-Hossein Abadi5 , M Hosseinzadeh6 , A Jamshid Zadeh2 , S Mohammadi Samani2 , M Omidi7 |
1- Department of Pharmacology, Shiraz University of Medical Sciences, Shiraz, Iran
2- Drug Science Research Center, Shiraz University of Medical Sciences, Shiraz, Iran,
3- Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran,
4- Student Research Committee, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran , asadi_427@yahoo.com
5- Laboratory Centers, Shiraz University of Medical Sciences, Shiraz, Iran,
6- Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran,
7- Department of Pharmacology and Toxicology, Hormozgan University of Medical Sciences, Bandar Abbas , Iran |
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Abstract: (7038 Views) |
Background & aim: Inflammatory bowel disease (IBD) as a chronic condition of the intestine with unknown etiology involving different agents like immune, genetic and environment. Today, many of herbal and synthetic compounds are being used as anti-inflammatory products around the world. The aim of this study was to investigate the anti-inflammatory effect of aquatic extraction of Teucrium polium L. plant on colitis in rat.
Methods: In this experimental study, a total of 135 male rats, weighing 200±20 g and 10 to 12 weeks old, were randomly divided into nine equal groups: healthy control group were given normal saline without ulcerative colitis (UC) induction, negative control group which UC was induced by 3% acetic acid with no treatment, basal gel group which were received basal gel via enema after UC induction, two groups as positive controls were taken Asacol (10 mg/kg/day) and Mesalamine (10 mg/kg/day) trans-rectally and orally, respectively. Also, oral form of aqueous extraction with doses of 50 and 100 mg/kg/day in two groups and enema form (gel 10% and 20%) of Teucrium polium L. in two groups were given. At the end of study, MDA measurement as antioxidant index and histopathological evaluation of colon tissue were performed. The collected data were analysed using one-way ANOVA and Mann–Whitney U test.
Results: Enema gels 10% and 20% lead to repair of damaged tissue of colon at the microscopic level and caused reduction in MDA level (p<0.01). Gels 20% compared with negative control showed significant difference in in terms ofMDA (p<0.01). Mesalamine and Asacol as positive controls, compared with each other and alsooral and enema forms of Teucrium polium L. showed no significant difference in respect of MDA (p<0.01). Although, enema gel form compared with oral form of plant aquatic extraction showed no significant difference, but enema form had better effect in control of inflammation.
Conclusion: Teucrium polium L. has remarkable effects on healing processing of induced colitis in male rat. According to anti-inflammation, antioxidant and anti-microbial properties of Teucrium polium L., it can be mentioned that this plant is effective in healing of intestinal ulcers.
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| Keywords: Inflammatory bowel disease, Teucriumpolium L, male rat, colitis |
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Full-Text [PDF 879 kb]
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Type of Study: Research |
Subject:
Special
Received: 2017/07/23 | Accepted: 2018/02/7 | Published: 2018/02/26
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Toneedeh N, Lashkarpoor H, Daneshchi S, Asadi Yousefabad S, Koohi-Hossein Abadi O, Hosseinzadeh M, et al . The Healing effect of Teucriumpolium L. on Acetic Acid-Induced Colitis in Male Rat. armaghanj 2018; 23 (1) :29-41 URL: http://armaghanj.yums.ac.ir/article-1-1887-en.html
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