[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 22, Number 4 (7-2017) ::
__Armaghane Danesh__ 2017, 22(4): 0-0 Back to browse issues page
The effects of hydro-alcoholic extract of Nigella sativa seed on sperm parameters and spermatogenesis in toxicity induced by diazinon in male rats
Dr Fereshteh Mir Mohammadrezaei 1, Miss reihaneh ghasemi2, Dr Sima Nasri3, Dr Amir Nili ahmadabadi4
1- assistant professor University of Mazandaran , fereshteh.mmrezaei@gmail.com
2- Master Science Payamenoor university
3- associate professor Payamenoor university
4- assistant professor Hamadan University of Medical Science
Abstract:   (128 Views)
Background & aim: Diazinon (DZN) is an organophosphate pesticide that can cause male infertility via the production of free radicals. Considering the antioxidant properties of Nigella sativa (NS) seed, the effects of hydro-alcoholic extract of NS seed were investigated on spermatogenesis in toxicity induced by DZN in male wistar rats. Methods: Thirty adult male rats were used and randomly divided into 6 groups (n=6): the negative control group received nothing, the sham group received corn oil and saline, the positive control group received DZN at a dose of 16 mg/kg body weight , the experimental groups 1 and 2 received DZN and doses of 100 and 200 mg/kg body weight NS extract; respectively and the experimental group 3 received a dose of 200 mg/kg body weight NS extract. All of treatment was done via gavage for two months. After 2 months, sperm parameters such as sperm count, motility and morphology of sperms were evaluated and the testicular tissue sections were stained by hematoxylin and eosin (H&E). Data were analyzed using Prism Software Version 5 and One-way ANOVA and Tukey ̕s post hoc test. Results: DZN (16 mg/kg) significantly decreased the number (P<0.01) and motility of sperms (P<0.001) compared to the sham group but didn’t significantly alter sperm viability and morphology compared to the sham group. The doses of 100 and 200 mg/kg body weight of NS extract increased sperms counts (P<0.01and P<0.001; respectively) compared to DZN group. NS did not significantly change the sperm motility in the presence of DZN. Histological studies showed the number of primary spermatocytes and spermatids significantly reduced in DZN group compared to the sham group) P<0.05). In treatment groups with the doses of 100 and 200 mg/kg of NS extract, a significant increase was observed in the number of spermatogonia, primary spermatocytes and spermatids compared to DZN group) P<0.05). In the experimental group 3 increased the number of sperm compared to sham group) P<0.05) but didn’t change testis tissue. Conclusion: The results indicated protective effects of NS extract on testis tissue which may increase spermatogonia, primary spermatocyte, spermatids and the number of sperms in toxicity induced by DZN in male rats but no change sperm motility.
 
Keywords: Diazinon, Nigella sativa, Sperm parameters, Testis
     
Type of Study: Research | Subject: Physiology
Received: 2017/04/8 | Accepted: 2017/09/4 | Published: 2017/09/28
Send email to the article author

Add your comments about this article
Your username or email:

Write the security code in the box >



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mir Mohammadrezaei F, ghasemi R, Nasri S, Nili ahmadabadi A. The effects of hydro-alcoholic extract of Nigella sativa seed on sperm parameters and spermatogenesis in toxicity induced by diazinon in male rats. Armaghane danesh. 2017; 22 (4)
URL: http://armaghanj.yums.ac.ir/article-1-1756-en.html
Volume 22, Number 4 (7-2017) Back to browse issues page
ارمغان دانش Armaghane danesh
Persian site map - English site map - Created in 0.052 seconds with 795 queries by yektaweb 3471