|
The Effects of Neonatal Administration of Tamoxifen and Zearalenone on the Features of Uterus Tissue in Mice
|
KH Sedaghat KH1   , R Parandin2 , M Behnam-Rassouli M 3, N Mahdavi-Shahri1 , M Kheirabadi1 , H Kalkali1 |
1- Department of Biology, Ferdowsi University of Mashhad, Mashhad, Iran
2- Department of Biology, Payame Noor University, Tehran, Iran
3- Department of Biology, Ferdowsi University of Mashhad, Mashhad, Iran , behnam@um.ac.ir |
|
|
Abstract: (5062 Views) |
Abstract
Background and aim: The uterus as the major female reproductive organ is particularly vulnerable to environmental and hormonal like compounds. Exposure to estrogen like disrupting chemicals during critical periods of development especially in the neonatal period can have adverse effects on reproduction and fertility later in life. In the present study, the effects of neonatal exposure of mycoestrogen zearalenone and anti-estrogen tamoxifen on some histological characteristics of the uterus in female mice were investigated.
Methods: 28 newborn female mice of BALB/c strain were divided into four groups of 7, three experimental and one control group. Treatment Groups included Tamoxifen (400 μg /kg /day) group, group treated with zearalenone (2 mg /kg /day) and group treated with tamoxifen + zearalenone. Control mice received no treatment. Daily treatments was started first day of life and continued up to 5 subcutaneously. Approximately, 70 days after birth in diestrous stage of estrous cycle, mice were deeply anesthetized and uterus was removed. Following histochemical staining, the samples were studied using light and transmission electron microcopies.
Results: The result of this study indicated that among the treatment groups, body weight decreased significantly (P>0.05) in the group which received Tamoxifen compared with control group. Uterus weight increased significantly in Zearalenone group (P>0.05), Tamoxifen and Zearalenone group (P>0.01). Endometrial thickness increased significantly (P>0.05) in group which received Tamoxifen and decreased significantly (P>0.05) in group which received Zearalenone. In addition, myometrial thickness decreased significantly (P>0.05) in group which received Zearalenone compared with control group. The study of electron microscopy showed that the number of cells of uterine luminal epithelial structure increased in the group treated with Tamoxifen compared with control group.
Conclusion: The result of study indicated that neonatal administration of Tamoxifen and Zearalenone compounds cause histological lesions in uterus structure. Because of the estrogenic nature of zearalenone, the treated neonatal mice with this mycotoxin cause structural abnormalities especially in myometrial structure. On the other hand, Tamoxifen as an estrogenic agonist cause endometrial thickness increase and alterations in the uterus.
|
|
| Keywords: Zearalenone, Tamoxifen, Uterus, Mice, Estrogen |
|
|
Full-Text [PDF 573 kb]
(1556 Downloads)
|
Type of Study: Research |
Subject:
Physiology
Received: 2016/07/3 | Accepted: 2016/08/22 | Published: 2017/03/15
|
|
|
|
|
|
|
| Send email to the article author |
|
|
|
| Add your comments about this article |
|
|
|
