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:: Volume 20, Issue 8 (11-2015) ::
__Armaghane Danesh__ 2015, 20(8): 720-731 Back to browse issues page
The Effect of Insulin-like Growth Factor-1, Cysteamine and β-Mercaptoethanol on the In Vitro Maturation of Immature Mice Oocytes
A Dehghan Manshadi1, M Rostami Hosseinkhani1, M Nikseresht2, A Hedayatpoor3, M Akbartabar toori4, S Mehrabi2, R Mahmoudi
1- 1Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran
2- Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
3- departmant anatomy University of Medical Sciences, Tehran, Iran
4- 4Social Determinants of Health Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
Abstract:   (2482 Views)

Background & aim: In vitro maturation of oocytes is a promising technique for reducing the costs and complications of ovarian stimulation by gonadotropins. The aim of this study was to investigate the effects of combination of insulin-like growth factor-1 and antioxidant cysteamine and &beta-Mercaptoethanol on maturation and fertilization of immature oocytes.

Methods: in this experimental study, following 48 hrs injection of 7.5 IU PMSG to immature female mice, the germinal vesicle oocytes from ovaries were removed and transferred to TCM199 culture medium containing 50 ng /ml insulin-like growth factor-1 and 100 &mumol Cysteamine and &beta -Mercaptoethanol. After 24 hrs of culture, the oocytes of MII in IVF were fertilized and embryonic development to the two cells was studied under an inverted microscope. Data analysis was performed by using ANOVA and Post hoc Tukey test.

Results: The results showed that the rate of maturation, fertilization and 2-cell embryo formation in GV oocytes with cumulus cells in TCM199 medium containing insulin-like growth factor-1, Cysteamine and BME were 92.10, 93.30, 80.60% and in the GV oocytes without Cumulus cells were cultured in the same medium were 65.80, 64.00, 58.60% respectively which were statistically significant (P <0.001).

Conclusion: In the present study, the simultaneous combination of insulin-like growth factor-1, &beta-Mercaptoethanol and CYS increased maturation, fertilization and developmental rate to 2-cells stage with cumulus cells more than the oocyte without cumulus cells to a greater extent. This represented the need of adding supplemental growth factors and antioxidants to the medium and is associated with cumulus cells.

Keywords: Germinal vesicle oocytes, insulin-like growth factor 1, Cysteamine, Beta-Mercaptoethanol, Cumulus cell, in vitro maturation
Full-Text [PDF 191 kb]   (809 Downloads)    
Type of Study: Research | Subject: Special
Received: 2015/11/17 | Accepted: 2015/11/17 | Published: 2015/11/17
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Dehghan Manshadi A, Rostami Hosseinkhani M, Nikseresht M, Hedayatpoor A, Akbartabar toori M, Mehrabi S et al . The Effect of Insulin-like Growth Factor-1, Cysteamine and β-Mercaptoethanol on the In Vitro Maturation of Immature Mice Oocytes. Armaghane danesh. 2015; 20 (8) :720-731
URL: http://armaghanj.yums.ac.ir/article-1-1135-en.html


Volume 20, Issue 8 (11-2015) Back to browse issues page
ارمغان دانش Armaghane danesh
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