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Abstract Background & aim: Diabetes is a metabolic disorder characterized by hyperglycemia resulting from perturbation in insulin secretion, action or both. The aim of this study was to investigate the effects of methanol extract of Teucrium orientale on blood glucose and liver damage markers in streptozotocin diabetic rats. Methods: In this experimental research 32 male albino Wistar rats, with body weights of 200 – 240 g were randomly allocated into four groups with 8 rats per each. Control group (normal rats), diabetic rats (received STZ in single dose 60 mg/kg bw, intraperitoneal way and without receive extract), treated normal rats (received T. orientale 200 mg/kg bw, oral gavage) diabetic treated rats (received STZ in single dose 60 mg/kg bw, intraperitoneal way and received T. orientale 200 mg/kg bw, oral gavage). Afterwards 21 days, blood glucose, malondialdehyde (MDA) in liver and the activity of the liver enzymes (ALT ،AST،ALP( were evaluated. The collected data were analyzed using SPSS software via the one-way ANOVA. Resulths: This study demonstrated that sSerum levels of glucose in T. orientale treated diabetic group (222 ± 9.8) were significantly lowerdecreased than in comparison with diabetic ratsgroup (572 ± 8) (P < 0.01).The MDA level in T. orientale treated diabetic group (1.01 ± 0.04) were significantly decreased in comparison with diabetic rats (1.25± 0.54) (P < 0.05). The ALT, AST and ALP levels in T. orientale treated diabetic group were also significantly decreased compared with diabetic rats (P < 0.01). Conclusion: Methanol extract of T. orientale had antidiabetic effects and consequently might alleviate the liver damage caused by streptozotocin-induced diabetes mellitus. Keywords: Diabetes, Teucrium orientale, ALT, AST, ALPLiver enzymes, streptozotocin

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ABSTRACT

Background & purpose: Hydatidosis is a zoonotic disease that caused by infection with the larvae of Echinococcus granulosus. Different antigens produced in larval stage of this parasite that recombinant vaccine base these antigens created significant immunity in infected animals. One of the important antigens is p14-3-3 that it's recombinant antigen created considerable immunity in mouse models. In this study according to the high immunity of antigen epitopes region the coding sequence of T-cell epitopes of P14-3-3 was cloned into pEGFP-N1vector in order to produce an effective DNA vaccine model to stimulate high level of Th1 immune response.

  Material and method: In this study bioinformatics tools were used to prediction of linear T-Cell epitopes of Echinococcus granulosus P14-3-3 &zeta antigen. The nucleotide coding sequence of these epitopes was synthesized by PCR. the ampliqon was digested with XhoI restriction enzyme and cloned into pEGFP–N1 vector That has been purificated by modified sambrook method with CaCl2 and PEG6000..Positive colony was selected by direct colony PCR and confirmed by the sequencing.and evaluation of it's expression in Eukaryotic cells was done by transformed to CHO cell line with electroporation.

Results: Linear T-cell epitopes of Echinococcus granulosus P14-3-3 after prediction,synthesis and amplification wae successfully cloned into pEGFP-N1 vector that purificated by new method with maximum vector and minimum RNA concentration.The expression of new constract in CHO cell line as a eukaryotic cells achivment by fluorescent microscope and will be used as a DNA vaccine model to evaluation immuno response in mouse models.

  Discussion: Successfully cloning of The linear T-cell epitppes coding sequence of Echinococcus granulosus P14-3-3&zeta antigen into pEGFP-N1 verificated by sequencing and fluorscent microscope images demonstrated expression of recombinant protein in CHO cell line

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Background & aim: Panton-Valentine leukocidin (PVL) is a Staphylococcus aureus gamma toxin. There may be a link between Meticillin-Resistant Staphylococcus aureus and Panton-Valentine leukocidin (PVL), as an important cytotoxin found particularly in severe infections. The purpose of this study was to isolate and identify Staphylococcus aureus virulence Panton-Valentine leukocidin (PVL) and methicillin resistance genes in clinical samples using PCR techniques.

Methods: In the present cross-sectional study, 89 isolates were collected from blood samples and detected as Staphylococcus aureus during the period of 6 months at Ali Ebne AbiTaleb Hospital, Zahedan, Iran. Initially, the case study examples were examined by biochemical tests. Then, based on recognized standards, Staphylococcus aureus isolates were isolated. Afterwards, isolates obtained were confirm by using 16srRNA gene. Subsequently, the antibiotic susceptibility of all isolates to methicillin was determined using Cefoxitin(30µg) disk diffusion and agar screening methods. Finally, the PCR method was used to determine PVL and mecA genes. All results were analyzed by the Chi-square test.

Results: Out of the total 89 isolates of Staphylococcus aureus blood isolates, 26 isolates from wounds and 63 were isolated. Most samples were obtained from female patients. According to the molecular analysis, 47 isolates (82/52%) were mecA gene and resistant to methicillin and 42 strains (47/2%) was methicillin-sensitive. Resistance to vancomycin wasn’t observed in isolates. Erythromycin and Penicillin had the highest prevalence of antibiotic resistance among isolates, respectively. Multi-resistant strains were the most PLV genes frequent. A significant relationship was observed between the resistance and the presence of Pantone Valentin (P≥0 / 05).

Conclusions: Due to the frequency of mecA and PLV genes in resistant and susceptible strains of Staphylococcus aureus, and also the distribution of Pantone Valentin gene in resistant strains; therefore, based on the statistical analysis, we can conclude that there may be a connection between these two factors (P≥0 / 05).

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Abstract:
 
Background and Aim:  Zinc phosphate is a solid gray chemical compound whose commercial sample is dark or even black and white. Severe hypertension and cardiac poisoning are the most serious complications of zinc phosphate poisoning and are associated with a high mortality rate. Poisoning with zinc phosphide as a rat poison, which has similar effects to aluminum phosphide tabletsis is uncommon. The report relates to a death case following poisoning with zinc phosphide mice.
 
Case Report: The patient was a young 18-year-old man who was admitted to suicide attempted at hospital. Due to the lack of toxic poison, the patient was treated with supravavalin as probable toxicity. After several hours of admission, his level of consciousness was reduced with deep breathing. In the tests after the onset of shortness of breath, pH was 6.94, HCO3: 4.9, blood sugar 21, creatinine 6.1 and BUN: 24. Then, the zinc phosphate was detected. Despite the onset of treatment, after 1 hour, the patient suffered from cardiopulmonary arrest and eventually died.
 
Conclusion: Poisoning with zinc phosphide can cause acute renal failure. Considering that phosphide is one of the poisonous toxins of rodents, especially in rat poison, toxic poisoning with phosphodies is also recommended in poisonous toxin poisoning cases.
 
 

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Background & aim: Human errors, which can be caused by factors such as mental and personality conditions, are the most important factors causing irreparable loss of life and property in traffic accidents. Depression is one of the most common and complex medical and social issues, and if left untreated, it can affect a person's abilities. The aim of this study was to determine and investigate the prevalence of background depression and related factors in drivers of passenger cars with an accident, Trauma Center.
 
Methods:The present study was a cross-sectional descriptive-analytical study of 195 drivers with a car accident in the first half of 2018 at Shahid Beheshti Hospital. Demographic information was collected by a questionnaire and depression was collected by a Beck questionnaire. The questionnaire took an average of 10 minutes to complete. The questionnaire was conducted by a physician who was familiar with the interview. The presence and severity of depression were measured based on this questionnaire and based on the score obtained from the Beck test in the category of very mild or normal depression (grades 1 to 10), mild (11 to 16), borderline and need for counseling (17 to 20), moderate or they were depressed (21 to 30), severely depressed (31 to 40) and overly depressed (41 to 63). Version 3.6.1 R software was used to analyze the data. Based on the type of data collected, statistical tests were used for student analysis, Kolmogorov-Smirinov, Spearman, Mann Whitney, Croscale Wallis, Fisher's meta-analysis or accurate test for analysis.
 
Results: The results indicated that the average age of drivers was 37.6 years. 55.36% of the study population were married, 46.67% had a university degree, 89.33% of the study population had a normal and low level of economic satisfaction and more than 60% of them did not have job satisfaction. The average depression score was based on the Beck 16 questionnaire, and only 28% of drivers were depressed and healthy, and about 14.36 percent had an alcohol consumption culture.
 
Conclusion: The results of the present study indicated that the prevalence of depression in drivers in Yasuj was high, out of 9 factors studied, only four factors of smoking, alcohol consumption, economic status and job satisfaction showed a significant correlation between depression and depression. Contrary to popular belief and culture that alcohol is not consumed, consumption among drivers with accidents was widespread.
 

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Background & aim: Pneumothorax is a common finding after trauma and with a wide range of clinical manifestations, from a concealed pneumothorax detectable only by a CT scan accidentally, to a potentially fatal tension pneumothorax. Pneumothorax can gradually progress to tension pneumothorax and become an emergency, consequently, a timely diagnosis is essential. Most traumatic patients have unstable conditions and are risky to transport for radiological evaluation, so they need a fast and bedside way of diagnosis. Currently, sonography is the only acceptable method which is available bedside. This study designed at this center to assess the diagnostic accuracy of ultrasound in trauma condition and comparing to that of CT scan as the final test for pneumothorax diagnosis.
 
Methods: in this cross-sectional prospective and analytical study in 2019, 290 patients were eligible for the study, fitted the Advanced Trauma Life Support (ATLS). A computerized tomography scan (CT) and extended Focused Assessment Sonography of Trauma (eFAST) done for each patient. Sonography performed while regarding the standards for the eFAST examination. During the sonography examination, the process of resuscitation not impaired or stopped. Conform to the entry and exit criteria of the study, data collected in a questionnaire and entered in R software version 3.6.1. A series of tests including Sensitivity, specificity, positive and negative predictive values (PPV, NPV), positive and negative likelihood ratios (LR+, LR-), ROC curve analysis, McNemar test, and the Kappa coefficient used for evaluation. The results compared with that of the CT scan as the definitive diagnostic method.
 
Results: from 290 patients enrolled in the study,  71% male and %29 were female. The mean age was 38.72 ± 16.7 years, and the age range was 14 to 78 years. The age distribution of the patients was not normal and skewed positively for 20 to 40 years old (median age 34.5 years old). The cause of trauma was non-penetrating in 99.3% and penetrating in 0.7%. According to the CT scan results as the final diagnostic method, 37 patients developed pneumothorax. Ultrasound sensitivity and specificity in pneumothorax diagnosis were 81.1% and 98.4%, respectively. The positive predictive and negative predictive for eFAST were 88.2% and 97.2%. Data fitness evaluated by the Kappa coefficient and McNemar test (Kappa of 0.82 and a p-value of 0.365). These tests showed a high concordance between the two diagnostic methods (eFAST and CT scan) for pneumothorax diagnosis. The area under the ROC curve for sonography was 0.898 and had a relatively good overlap with that of the ROC curve for CT scan.
 
Conclusion: In general, extended Focused Assessment Sonography of Trauma is valuable in the diagnosis of pneumothorax with a diagnostic value close to CT scan. If positive for pneumothorax, the result is precise, but when negative, additional assessment required due to a lower sensitivity. It could also be a better substitute for a simple chest x-ray.
 
 

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Background & aim: SARS-CoV-2 virus expresses specific patterns of genetic diversity in the genome. These changes in the virus and its genetic diversity in human populations can determine the transmission of the virus and the severity of the Covid-19 disease. Genetic diversity and immune differences in human populations can be the driving force in the evolution of the virus and its escape from the immune system. The aim of the present study was to investigate the genetic changes and escape routes of the SARS-CoV-2 virus from immune responses.

Methods: In this review article conducted in 2021 at Yasuj University of Medical Sciences, studies published in electronic databases; Pub-Med, Science Direct, Elsevier, Scopus and Google Scholar were investigated regarding the genetic diversity of the SARS-CoV-2 virus and its ways of escaping the immune system.

Results: The results indicated that various mutations have been observed in SARS-CoV-2 virus types, some of these mutations are effective on viral characteristics such as infection and severity of the covid-19 disease and are related to the ability to escape from immune responses and can lead to inhibition of innate immune responses. and be acquired.

Conclusion: By identifying the types of mutations of SARS-CoV-2 virus and their relationship with the ways of escaping from the immune system, we can hope for effective treatment methods as well as obtaining vaccines with greater efficiency and effectiveness.