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Rapid Detection of Pseudomonas Aeruginosa by PCR Method Using Specific Primers of Quorum Sensing LasI gene
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H Aghamollaei   , K Azizi Barjini , M Moosazadeh Mogaddam 1 |
| 1- , mm.genetics@gmail.com |
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Abstract: (14517 Views) |
| Abstract
Background & aim: Pseudomonas aeruginosa is an opportunistic nosocomial pathogen that due to having intrinsic and acquired resistance to common antibiotics, mortality due to infections is very common. Therefore, early and accurate identification of bacteria could be effective in controlling infections and deaths. The aim of this study was to evaluate the use of a rapid method with high sensitivity and specificity based on polymerase chain reaction using gene-specific primers quorum sensing LasI system for detection of bacteria.
Methods: In this study, the comparison between the results of culture and PCR for the diagnosis of Pseudomonas aeruginosa in clinical samples and other bacterial species were carried out. 40 strains of Pseudomonas aeruginosa from isolated clinical specimens were identified and confirmed by biochemical tests. LasI gene specific primers were designed using bioinformatics analysis. Sequence of this gene was amplified techniques after extraction of bacterial genome. The specificity of PCR tests with DNA from of different species, Staphylococcus aureus, Klebsiella pneumonia, Escherichia coli and Vibrio cholerae were evaluated. A different dilution of the bacterial genome of Pseudomonas aeruginosa was used in PCR to evaluate the sensitivity of primer. Data were analyzed by t-test
Results: The results indicated that the PCR test result was positive for all strains of P. aeruginosa isolates however, PCR test results were negative for the four other bacteria. Even at 10-5 Pseudomonas aeruginosa genome concentration, PCR test was positive for all isolated strains
Conclusion: This study showed that the primers designed for detection of Pseudomonas aeruginosa using PCR, had higher sensitivity and specificity compared to previous methods.
Keywords: Pseudomonas aeruginosa, PCR, Quorum sensing, LasI |
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| Keywords: Pseudomonas aeruginosa, PCR, Quorum sensing, LasI |
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Full-Text [PDF 881 kb]
(3949 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2015/05/6 | Accepted: 2015/05/6 | Published: 2015/05/6
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