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In Vitro Antibacterial Activity of Teucrium Polium L. Hydroalcoholic Extract Against Standard and Clinical Strains of Escherichia Coli Compared with Conventional Antibiotics
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SJ Hedayat1   , H Ebrahimi1 , A Sharifi2 , O Eilami3 , Z Saeedinejad4 , SM Khosravani1 , SA Khosravani5 |
1- Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran,
2- Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
3- HIV/AIDS Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
4- Department of Internal Medicine, Yasuj University of Medical Sciences, Yasuj, Iran
5- Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran , khosravani2us@yahoo.com |
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Abstract: (1425 Views) |
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Armaghane-danesh, Yasuj University of Original Article
Medical Sciences Journal (YUMSJ)
In Vitro Antibacterial Activity of Teucrium Polium L. Hydroalcoholic Extract Against Standard and Clinical Strains of Escherichia Coli Compared with Conventional Antibiotics
Hedayat SJ1, Ebrahimi H1, Sharifi A2, Eilami O3, Saeedinejad Z4, Khosravani SM1, Khosravani SA2*
1Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran, 2Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran, 3 HIV/AIDS Research Center, Shiraz University of Medical Sciences, Shiraz, Iran, 4Department of Internal Medicine, Yasuj University of Medical Sciences, Yasuj, Iran
Received Date: 13 Apr 2025 Accepted Date: 13 Oct 2025
Abstract
Background & aim: Urinary tract infections (UTIs) are among the most prevalent infectious diseases, frequently caused by Escherichia coli. The escalating antibiotic resistance of this bacterium, driven by widespread antibiotic use and the transfer of resistance genes, has intensified therapeutic challenges. Teucrium polium L. rich in bioactive compounds such as monoterpenes and polyphenols, has been valued in traditional medicine. Therefore, the aim of this study was to determine and evaluate the antibacterial activity of the hydroalcoholic extract of Teucrium polium L. against standard and clinical strains of Escherichia coli in comparison with conventional antibiotics.
Methods: This is an experimental study in which the hydroalcoholic extract of Teucrium polium L. was prepared by the maceration method from plant samples collected in the city of Yasuj. The standard E. coli strain (ATCC 25922) and clinical isolates from UTI patients were tested. The susceptibility of the strains to antibiotics, including nitrofurantoin, gentamicin, cotrimoxazole, cefepime, ceftriaxone, and ciprofloxacin, was assessed using the disk diffusion method. The antibacterial activity of the extract was evaluated by determining the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) via the microdilution method, and the zone of inhibition (ZOI) diameter. The collected data were analyzed using the Kolmogorov–Smirnov test, one-way ANOVA, independent t-test, and repeated measures analysis of variance.
Results: Both standard and clinical E. coli strains were susceptible to the tested antibiotics. The MIC of the T. polium extract was 62.5 μg/mL for the standard strain and ranged from 31.25 to 62.5 μg/mL for clinical isolates. The MBC ranged from 125 to 250 μg/mL. No stastically significant differences were observed between the MIC and MBC of standard and clinical strains (pvalue>0.05). The ZOI diameter was larger for the standard strain compared to clinical isolates, though this difference was not statistically significant (P.value>0.05). The T. polium extract exhibited considerable antibacterial activity, albeit less potent than some antibiotics.
Conclusions: The hydroalcoholic extract of T. polium demonstrates antibacterial potential against E. coli and could serve as a natural adjunct to address antibiotic resistance. Further studies are recommended to investigate its toxicity and identify active compounds. These findings underscore the significance of medicinal plants in developing novel therapeutic strategies.
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| Keywords: Urinary Tract Infections, Teucrium polium, Escherichia coli, Plant Extracts, Drug Resistance |
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Type of Study: Research |
Subject:
Microbiology
Received: 2025/04/13 | Accepted: 2025/10/13 | Published: 2025/11/29
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Hedayat S, Ebrahimi H, Sharifi A, Eilami O, Saeedinejad Z, Khosravani S et al . In Vitro Antibacterial Activity of Teucrium Polium L. Hydroalcoholic Extract Against Standard and Clinical Strains of Escherichia Coli Compared with Conventional Antibiotics. armaghanj 2026; 31 (1) :34-49 URL: http://armaghanj.yums.ac.ir/article-1-3790-en.html
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