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The Effect of Hydroalcoholic Extract of Pistacia atlantica Fruit (Bene) on Clinical Isolates of Candida albicans from Patients with Denture Stomatitis Compared to Nystatin
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R Baziar1   , F Khajehzadeh2 , M Ghorghani3 , E Panahi Kokhdan3 , H Bardania4 |
1- Department of Dentistry, Yasuj University of Medical Sciences, Yasuj, Iran,
2- Department of Oral and Maxillofacial Pathology, Yasuj University of Medical Sciences, Yasuj, Iran , khajezadehfatemeh3@gmail.com
3- Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
4- Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran |
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Abstract: (1912 Views) |
Background & aim: Denture stomatitis is the most common oral fungal infection in the elderly, primarily caused by Candida albicans. The present study aimed to investigate the antifungal activity of the hydroalcoholic extract of Pistacia atlantica fruit (bene) compared to nystatin against clinical isolates of Candida albicans obtained from patients with denture stomatitis.
Methods: The present in vitro descriptive-analytical study was conducted in 2023. Samples were collected from 19 elderly patients with denture stomatitis. Candida isolates were identified and confirmed by culture on Sabouraud dextrose agar, CHROMagar Candida, germ tube test, and growth at 42°C. Ultimately, 10 isolates of Candida albicans were obtained. The hydroalcoholic extract of bene fruit was prepared using the maceration method, and susceptibility testing was performed by the broth microdilution method (CLSI M27-S4) in RPMI 1640 medium in triplicate. Data were analyzed using Shapiro-Wilk, Friedman, and Wilcoxon tests via SPSS software version 20.
Results: The MIC range for the bene extract was 1–2 mg/mL, with MIC₅₀ of 1 mg/mL and MIC₉₀ of 2 mg/mL. The MIC range for nystatin was 0.125 to more than 4 μg/mL, with MIC₅₀ of 0.125 μg/mL and MIC₉₀ of 2 μg/mL. The difference in MIC between the two compounds was statistically significant (Wilcoxon test, p=0.004).
Conclusion: The hydroalcoholic extract of Pistacia atlantica fruit exhibits notable antifungal activity against clinical isolates of Candida albicans, although it is significantly less potent than nystatin. This extract could be considered as a supplement or a basis for further investigation in the development of topical herbal antifungal pr |
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| Keywords: Stomatitis, Denture, Candida albicans, Plant extracts, Nystatin |
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Full-Text [PDF 528 kb]
(23 Downloads)
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Type of Study: Applicable |
Subject:
Dentistry
Received: 2024/12/20 | Accepted: 2025/12/21 | Published: 2026/02/3
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