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:: Volume 22, Issue 1 (4-2017) ::
__Armaghane Danesh__ 2017, 22(1): 104-117 Back to browse issues page
Single Nucleotide Polymorphisms of MMP2 Gene Promoter on the Risk of Development and Metastasis of lung Cancer
H Keshvary Ravan1, F Kouhkan 2, H Sazgar1
1- Department of Biology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2- Department of Molecular Biology and Genetic Engineering, Stem Cell Technology Research Center, Tehran, Iran , biology1991@yahoo.com
Abstract:   (3768 Views)



Background & aim: The high incidence and poor prognosis of the lung cancer makes it aa a major health problem in the last few decades. Determination of frequency of different histopathology types of primary lung cancer has great importance in creating integrated treatment programs and recognized the effective factors causing the disease. Overexpression of MMPs has a direct relation with invasion and metastasis of malignant tumors in different tissues. The aim of this study was to assess the effect of MMP-2 gene promoter polymorphism with lung cancer and metastases in patients with lung cancer and compared with the control groups by the PCR-RFLP method.


Methods: In the present case-control study, The MMP-2 polymorphisms were analyzed by restriction fragment-length polymorphism (RFLP) in 50 patients with lung cancer and 77 cohort sample. All samples were taken under supervision of a physician. DNA isolation was performed using DNA extraction kit (Cinnagen, Iran). MMP9 gene was amplified by specific primers and PCR product was digested with FSPBI restriction enzyme. Data were analysis using Chi square by the SPSS software.


Results: The examination of allelic and genotypic distribution in patients with lung cancer and control showed that the allele frequency of C and T in patients with lung cancer were 90 and 10% (P=0.04) and in the control were 80.15 and 30% (P=0.05) respectively. Also genotype frequency of CC, CT and TT in patients with lung cancer were 82, 16 and 2 (P=0.05) and in the control were 69.93, 31.16, 3.1 percentage respectively (P=0.5). No significant difference was seen in comparison of genotype groups in non-metastatic and control. Comparison of homozygous CC genotype and control were confirmed the direct involvement of c allele in metastasis


Conclusion: It seems that individuals with C allele can increase susceptibility to lung cancer. Also these findings indicate that CC genotype as a risk factor facilitating the spread of metastasis of lung cancer.


Keywords: Lung cancer, Matrix metalloproteinase, MMP-2, PCR RFLP
Full-Text [PDF 337 kb]   (1254 Downloads)    
Type of Study: Research | Subject: Clinical
Received: 2016/11/29 | Accepted: 2017/04/21 | Published: 2017/05/5
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Keshvary Ravan H, Kouhkan F, Sazgar H. Single Nucleotide Polymorphisms of MMP2 Gene Promoter on the Risk of Development and Metastasis of lung Cancer . Armaghane danesh. 2017; 22 (1) :104-117
URL: http://armaghanj.yums.ac.ir/article-1-1601-en.html

Volume 22, Issue 1 (4-2017) Back to browse issues page
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