[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
Indexing & Abstracting::
Publication Ethics::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Indexing & Abstracting
DOAJ
GOOGLE SCHOLAR
..
:: Search published articles ::
Showing 7 results for Candida Albicans

B Bonyadpour , M Akbarzdeh, K Pakshir, Aa Mohagheghzadeh,
Volume 14, Issue 2 (7-2009)
Abstract

ABSTRACT: Introduction & Objective: It has been estimated that up to 75% of women in their child-bearing age have been affected by vulvovaginal candidiasis at least once in their life time. Almost 45% of women experience this infection two or more times. The antifungal azole group, in topical and oral forms, is the common way of therapy. Herbal products are often used for vulvovaginal therapy. Nowadays, Toucrium polium (TP) products are being used as traditional medicine to reduce signs of Candida vaginitis. There is no study regarding to antifungal activity of TP smoke product in Iran. The aim of this study was to evaluate the In vitro activity of TP smoke product against Candida, isolated from women with Candida vaginitis, compared with antifungal drugs which are ordinary used to cure Candida vaginitis. Materials & Methods: The present study was conducted at the University of Medical Sciences of Shiraz in 1387 (2008). During seven months, samples were taken from 450 patients suffering from urogenital infections and 105 Candida vaginitis were detected. Germ tube test was used for identification of fungal species. TP smoke product was prepared in suitable potency. Antifungal activity of fluconazole, clotrimazole and TP product were evaluated by disk diffusion method. Sterile blank disks were loaded by TP smoke product in potency of 10-240 microliter/disk. Inhibition zone around the disks were measured and compared with each other. Results: 105 Candida species were isolated from the patients. Candida species were identified by germ tube test as Candida albicans 74 (70.5%) and Candida non-albicans 31(29.5%).The mean of inhibition zone around the clotrimazole disks was 22±5.39 along with one case of resistance. Forty seven species had resistance to fluconazole while 94% and 55.2.% of all samples were sensitive to clotrimazole and fluconazole respectively. All of the clinical isolates and standard Candida species were sensitive to TP smoke product. Conclusion: Considering the resistance of Candida species to antifungal drugs and good antifungal activity of TP smoke product, it is necessary to analyze the main compounds and chemical contents of TP extract for better evaluation of its antifungal activity. Keywords: Clotrimazole, Fluconazole, Candida Vaginitis, Candida Albicans, Toucrium Polium
F Haghighi , Sh Rodbar Mohammadi , P Mohammadi , M Eskandari,
Volume 15, Issue 2 (7-2010)
Abstract

Introduction & Objective: In recent years, the incidence of opportunistic fungi has shown a marked increase. Infection caused by common pathogenic fungi is a significant health problem in immune compromised hosts. The present study evaluated antifungal activity of Titanum dioxide nanoparticles and Ethylene Diamine Tetra-acetic Acid against Candida albicans as self-cleaning agent by standard micro dilution test. Materials & Methods: The present study was conducted at the Medical University of Tarbiyat Modares in 2009. TiO2 nanoparticles were obtained through the hydrolysis of TiCl4 (Titanium tetrachloride). Size and type of these nanoparticles were characterized by scanning electron microscopy (SEM) and X-Ray-Diffraction (XRD). Afterwards, the Minimum Inhibitory Concentration (MIC) and Minimal Fungicide Concentration (MFC) test for TiO2 and EDTA were performed. Results: Concentration of synthesised TiO2 was 7.03 mg/ml and 5.63 5.63 ×1020 particles/ml. Evaluation of morphology and diameter of the TiO2 nanoparticles with SEM showed that nanoparticles were spherical with diameter between 40-65 nm. MIC50 of 2.2, 1.24 and 0.125 µg/ml respectively. MIC90 and MFC of TiO2, EDTA and fluconazole were 3.51, 2.48 , 0.5 µg/ml and 4.06, 3.1 ,1 µg/ml respectively. Conclusion: In the present study, using of synthesized TiO2 nanoparticles with chemical method showed a suitable activity against Candida in comparison with Fluconazole. Thus it might represent a good candidates in elimination of Candida in medical from medical devices. Key Words:
E Farahbakhsh, Mh Yadegari , M Rajabi Bazl, M Taghizadeh Armaki,
Volume 16, Issue 3 (8-2011)
Abstract

Introduction & Objective: Nowadays, opportunistic fungi especially Candida albicans are the most common cause of life-threatening infections in immunodeficiency patients. Increasing Azole-resistant strains of C.albicans are a main problem in human immunodeficiency virus-infected patients. The aim of this study was to evaluate the CDR2 gene in C.albicans azole resistant strains, isolated from AIDS patients with oropharyngeal candidiasis by RT-PCR method. Materials & Methods: The present experimental study was conducted at Tarbiat Modares University of Medical Sciences in 2009. C. albicans isolates from HIV infected patients were identified by standard procedures, including germ tube formation, clamidospor and color of colonies on CHROM agar. At first, susceptibility of C. albicans isolates was assessed by disk diffusion agar technique. Then, CDR2 resistance gene was analyzed by RT-PCR and electrophoresis of the PCR products. Finally, patterns of the resulted bands were compared with standard fluconazole resistant strains. The collected data was analyzed using the SPSS software. Results: The results of drug sensitivity of 66 C. albicans isolates from AIDS patients revealed that 62.6% were susceptible, 8.6% were susceptible-dose dependent (SDD) and 28.7% were resistant. In RT-PCR analysis, 6% of patients had the CDR2 gene. Conclusion: The use of phenotypic methods like disk diffusion agar, which is cheaper, along with genotypic methods, like RT-PCR, which provide the possibility of studying the mechanism of drug resistance, is recommended.
A Ownagh, M Adibhesami ,
Volume 17, Issue 3 (8-2012)
Abstract

Background & aim: Propolis is a resinous substance collected by honeybees from buds and leaves of trees and plants, possessing various biological properties. This study aimed to assess the anti-fungal activities of Ethanolic extract of Propolis (EEP) in an experimental infection of vaginal candidiasis. Methods: In this study, twenty-four female rabbits were used for experimental infections. Animals were randomly divided into six equal groups: positive control group, negative control group, and four treatment groups. The four test groups were given doses of 125, 250, 500 and 1000 µg/ml of Propolis Ethanolic extract respectively while the positive control group received the standard Nystatin treatment and negative control group were given saline, locally, two times a day for 20 days. The data were analyzed, using descriptive statistics. Results: Among all groups, the results showed that dose of 1000 µg/ml of EEP were the most effective dose in treatment of experimental vaginal candidiasis. Clinical signs of vaginal candidiasis were healed in Nystatin group after 9 days. No effect was recorded after 20 days treatment of infection for others doses of EEP that were used in this study. Conclusion: It can be concluded that Ethanolic extract of Propolis in 1000 µg/ml concentration could treat experimental vaginal candidiasis in rabbits in a shorter time in comparison with standard nystatin treatment..
M Azizi Lari , S Hajimaghsoodi , Aa Jafari , Mh Akhavan Karbassi , M Dehghan,
Volume 27, Issue 5 (8-2022)
Abstract

Background & aim: Denture stomatitis is the most common opportunistic infection caused by candida in denture wearer’s palate, which is more common and severe among diabetic patients. Several studies have already been conducted to examine various drugs for treatment of candidiasis. Recently, some cases of drug resistance of none albicans Candida species to antifungal drugs have been reported. The aim of the present study was to compare the in vitro susceptibility of isolated Candida species of uncontrolled diabetic patients against Nystatin and Fluconazole.

Methods: In the present lab trial study, 43 patients with uncontrolled diabetes mellitus and denture stomatitis referred to Yazd Diabetes Research Center and Clinics were screened and sample were collected from the palate using sterile swabs. At that point samples were cultured to determine the differential colony counts through the color difference between the colonies and furthermore MIC of Nystatin and Fluconazole by two different methods (Broth microdilution &Disk diffusion). Data were analyzed using SPSS23 and Kruskal-Wallis test.

Results: In the present study, the most commonly isolated species were: Candida albicans (38/3%), followed by Candida tropicalis (14/9%), Candida krusei (10/6%), Candida glabrata (8/5%) and other species (27/7%). According to both methods, Candida albicans was the most sensitive to both Nystatin and Fluconazole. The most resistant species to Nystatin and Fluconazole were Candida glabrata and Candida Krusei respectively. In 100% concentration, sensitivity of candida species to Nystatin was significantly higher than Fluconazole, except for Candida albicans (p-value=0/05).

Conclusion: The present study indicated that Nystatin was better than Fluconazole for treatment of denture stomatitis in uncontrolled diabetic patients.

 
M Garghani , A Asfaram , N Roustaee, S Ansari , F Rahmani , H Taheri , Aa Saadat Nia , S Nooripoor Sisakht ,
Volume 29, Issue 4 (7-2024)
Abstract

Background & aim: Currently, due to the widespread use of antifungal drugs and the inability to treat candida patients with infection, the recurrence of the disease has increased in patients; therefore, this has caused researchers to use medicinal plants to treat fungal diseases. The aim of the present srudy was to investigate the effect of methanolic extract of Nasturtium officinale and Salvia officinalis on the clinical and standard isolates of Candida in comparison with the antifungal drug fluconazole.

Methods: In the present experimental study conducted in 2019 on 31 clinical and standard Candida isolates, including 11 Candida albicans isolates, 10 Candida glabrata isolates, and 10 Candida parapsilosis isolates isolated from candidal vaginitis, urinary tract infection, and otomycosis in Yasuj, Iran. Once collecting sage and spring grass, their hydroalcoholic extracts were prepared and used to determine fungal sensitivity by broth microdilution method. For this purpose, according to the standard yeast suspension dilutions along with each of the medicinal agents and plant extracts were added to the 96-well plates and incubated at 35°C. To conclude, the lowest concentration of growth inhibitor was calculated for each isolate. The collected data were analyzed using Excel software.

Results: In the present study, among the three drugs and extracts examined, the MIC50 for hydroalcoholic extract of sage on three species of Candida albicans, Candida glabrata and Candida parapsilosis, respectively; 25,000, 12,500 and 12,500 μg/ml and MIC50 for the methanol extract of spring grass on three species in the same way, 25,000 μg/ml and MIC50 for fluconazole for three species; 0.25, 0.5 and 0.125 μg/ml were obtained respectively.

Conclusions: Compared to fluconazole, hydroalcoholic extracts of sage and spring grass had much less effect on clinical and standard Candida isolates. The common species that causes candidiasis is Candida albicans, and according to the results of the presnt study, the hydroalcoholic extracts of sage and spring grass have relatively little effects in laboratory conditions; therefore, the use of this plant extract for the treatment of candidiasis is not recommended.

 
M Zare , J Razmara, S Parvizpour , Am Barzegari ,
Volume 29, Issue 4 (7-2024)
Abstract

Background & aim: Despite being considered a common yeast among humans, Candida albicans can become an invasive agent under certain conditions and cause various types of acute or chronic infections. The use of existing antifungal drugs is limited due to their side effects and the resistance of this yeast to them. Previous in vitro studies have demonstrated that thyme plant possesses antifungal properties and its metabolites have the ability to kill the candida strains resistant to azole antifungal drugs. Secreted aspartyl protease 5 (SAP5) plays a crucial role in the pathogenicity of this yeast. Therefore, the aim of the present study was to determine and investigate the molecular docking of biological compounds of garden thyme (L. Thymus vulgaris) with secreted aspartyl protease-5 of Candida albicans yeast to find possible inhibitory compounds.

Methods: In the present molecular docking study conducted in 2024, the active phytochemical compounds of the Thymus vulgaris plant were obtained from the LOTUS and NPASS databases. The structure of the SAP5 protein was retrieved from the RCSB PDB database. Molecular docking of ligands with the SAP5 enzyme was performed using AutoDock Vina in the PyRx 0.8 software package to calculate binding energies and determine the docking position of each compound in its interaction with SAP5. The compounds with the best binding energy to the target protein were further evaluated for pharmacokinetic properties and toxicity, and the selected ligands were visualized using BIOVIA Discovery Studio. The collected data were analyzed using different software and compared with the results of previous articles in this field.

Results: The binding energies of Thymus vulgaris compounds to the active site of the SAP5 enzyme ranged from -9.9 to -3.4 kcal/mol. The highest binding affinities to the active site were observed for the compounds Eriodictin, (-)-Taxifolin, and Ellagic Acid. These compounds also demonstrated favorable pharmacokinetic and toxicity profiles. Pharmacokinetic studies of three selected compounds using the SwissADME online server showed their promising medicinal properties. Despite this, investigating their toxicity using ADMETlab and ProTox-II servers showed the toxicity of eriodictin.

Conclusion: The results of the present study indicated that some compounds in thyme plant may inhibit SAP5. Therefore, probably one of the mechanisms involved in the anti-candidal effects of garden thyme on Candida albicans yeast is through the inhibition of this protein.

 

Page 1 from 1     

ارمغان دانش Armaghane Danesh
Persian site map - English site map - Created in 0.06 seconds with 33 queries by YEKTAWEB 4714