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Showing 2 results for Negintaji
Z Arsalan, A Asfaram , I Ghitasi, F Bizhani, K Negintaji , M Jafari Barmak, A Ghanbari,
Volume 27, Issue 3 (4-2022)
Abstract
Background & aim: Steroid production has been reported in the asexual tissues of the nervous system. Stimulants are in the normal activity, function and function of the nervous system. Identifying the conduction pathways involved in glucocorticoids and enabling brain parenchymal cells can offset the balance in the active nervous system at old ages when the body is depleted. Therefore, in this study, by increasing the activity of sonic hedgehog (SHH) pathway attempted by purmorphamine and its capacity by Gant 61, the effect of this pathway on steroid process in culture medium of glial neurons is evaluated.
Methods: The present experimental study was conducted in 2021. First, neuronal stem cells were obtained from the cortex of a 14-day-old embryonic mice by standard methods. Survival of neuronal stem cells after treatment with 5 μM pregnenolone with different concentrations of purmorphamine (1,2,5, 10 and 20) and Gant 61 was performed by MTT method. Then the cells were placed in a differentiation medium and after treatment with different concentrations and 5-day incubation, the surface of the cells was removed from the cell culture medium and the amount of testosterone and estrogen were measured by ELISA and HPLC. Data were analyzed using ANOVA statistical test using graph pad software.
Results: The survival data of the groups indicated an increase in survival after treatment with purmorphamine (114.3) compared to the Gant 61 group (63.7 Pg) (p≤0.5). Progesterone data in the supernatant of glial neurons showed that purmorphamine groups (287.2 Pg) had a significant increase compared to control (88.28) and Gant 61 (40.5 Pg) groups (p≤ 0.001). Also, testosterone data show that purmorphamine groups (73.8 Pg) in both ELISA and HPLC methods have a significant increase compared to the control (153.8 Pg) and Gant61 (52.92 Pg) groups (p≤ 0.0001). Also, pregnenolone group (236.5 Pg) showed a significant increase compared to Gant61 (40.5 Pg) group (p≤ 0.05). Analysis of estrogen data by HPLC method showed that there was a significant increase in estrogen production in the purmorphamine groups (331.2 Pg) compared to the control (42.11 Pg) and Gant61 (42.11 Pg) groups (p≤ 0.0001).
Conclusion: The data from this study indicated that the induction of the shh pathway by purrmorphamine increased the production of steroid hormones (estrogen-progesterone and testosterone) by glial-neuronal differentiating cells, which inhibition of this pathway had the opposite effect. The present study concluded that induction of the shh pathway can lead to the production of steroids.
K Negintaji , M Foroozanfar , M Jafarinia , A Ghanbari ,
Volume 28, Issue 5 (9-2023)
Abstract
Background & aim: Pregnenolone acts as a precursor to other steroid hormones and exerts its effect as an anti-inflammatory molecule to maintain immune homeostasis in various inflammatory conditions. In these diseases, a decrease in the level ofP has been observed, which emphasizes its role in neuroprotection and nerve regeneration and its anti-inflammatory role. Accordingly, the purpose of the present study was to determine the ability of Pregnenolone in the proliferation of mouse neural stem cells and reduce inflammatory and oxidant markers. of inducing inflammation with lipopolysaccharide in laboratory conditions.
Methods: In the present experimental study conducted at Yasuj University of Medical Sciences, neural stem cells (NSCs) were isolated from the embryonic cortex of E14 mice with standard protocol and incubated for 5 days. Subsequently, neurosphere formation and propagation for second passage the survival of the cells was done after pregnenolone combined treatment with lipopolysaccharide (LPS) inflammatory model. The number of neurospheres and cells derived from neurospheres were counted after 5 days of incubation in the inflammatory model. The supernatant of the cells was removed and the levels of oxidant and antioxidant markers MDA, NO FRAP, and inflammatory markers IL6 and TNFα were measured by ELISA method. Data were analyzed by one-way variance statistical method and Tukey's post hoc test.
Results: The results indicated that pregnenolone with its effect on inflammatory factors could increase the proliferation of neural stem cells in conditions of inflammation and the greatest effect was observed in the group treated with 10 μM dose of pregnenolone with an increase of 68% compared to the LPS group. On the other hand, it caused a decrease in the inflammatory factors TNF-α (12%) and IL-6 (30%) and oxidative stress factors including NO (38%) and MDA (20%) compared to the LPS group, as well as a significant increase FRAP was an antioxidant marker (P<0.0001) in the model of inflammation caused by LPS in the culture medium of mouse neural stem cells.
Conclusion: The results of the present study indicated that Pregnenolone, by affecting inflammatory factors, increased the proliferation of neural stem cells in the conditions of inflammation, and it was as well able to reduce the amount of inflammatory and oxidant markers in the inflammatory model of the culture medium.
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