S Afshar Yousefi , Y Kazemzadeh, H Shirvani , S Mirzayan Shanjani ,
Volume 29, Issue 5 (10-2024)
Abstract
Background & aim: Lifestyle modification and the use of nanotechnology are considered therapeutic strategies for cancers. Therefore, the aim of the present study was to determine the effect of combined exercise and curcumin supplementation on the expression of T cell factor 1 (TCF-1) and glycogen synthase kinase 3 beta (GSK3β) genes in the brains of mice with glioblastoma multiforme.
Methods: In the present experimental study conducted in 2023 at the Islamic Azad University, 40 male Wistar rats were divided into 5 healthy control groups, glioblastoma multiforme (GBM), GBM+ combined training (CT), GBM+ nanocurcumin supplement (N-CUR) and GBM+CT+N-CUR (8 rats in each group). Glioblastoma was injected into the frontal cortex of rat. Exercise training was performed for 4 weeks and 3 days per week as a combination of aerobic and resistance training. Nano curcumin supplement was gavage with a dose of 80 mg/kg for 4 weeks, 5 days per week. At the end, rats sacrificed and TCF-1 and GSK3β genes of brain glioblastoma were analyzed by Real-time PCR method. The collected data were analyzed using Shapiro-Wilk, Levin, one-way analysis of variance, Tukey's post hoc test and Pearson correlation.
Results: The results of the present study indicated that the expression of TCF1 mRNA and GSK3β mRNA in the brain tumor tissue of rats showed a significant increase compared to the healthy control group (p<0.0001 for both). However, compared to the GBM group, all the treatment groups, especially the GBM+CT+N-CUR group, indicated a significant decrease in the expression of TCF1 mRNA and GSK3β mRNA in the brain tumor tissue (p<0.05). The correlation between these two factors was not confirmed in different research groups (p>0.05). Based on histological image, tumor induction caused tissue integrity destruction and the level of inflammatory factors and lymphocytes infiltration in this tissue also expanded. However, exercise training and nano curcumin supplementation led to control of tumor tissue damage and reduction of tumor tissue, and these changes were greater in combination therapy.
Conclusion: It seemed that the combination of exercise and Nano curcumin supplement could be an effective treatment method in down regulation of some effective genes in brain tumor tissue. Therefore, they can be used during the recovery period or cancer treatment with the doctor's order. However, in this field, more studies are needed, especially in human samples.
