[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 20, Issue 11 (2-2016) ::
__Armaghane Danesh__ 2016, 20(11): 985-995 Back to browse issues page
Effect of hydroalcholic extract of Cynodon dactylon the phagocytosis and respiratory burst of peritoneal macrophages of NMRI mice
SM Abtahi Froushani
Urmia auniversity , meysamabtahi@hotmail.com
Abstract:   (4076 Views)

Background: The anti-inflammatory effects of Cynodon dactylon have been determined in some previous studies. Nevertheless, there is no comprehensive study about immunomodulatory effects of C. dactylon. The present study was performed to investigate the modulatory effects of C. dactylonon macrophages functions of NMRI mice.

Methods: This survey was an experimental intervention study. The  study  population  consisted  of  14  male  NMRI  mice  randomly  categorized  into  3 treatment groups and one control group (each group was contained 10 mice). Mice in treatment groups received hydroalcoholic extract of C. dactylon for 3 constitutive weeksin different doses (100,200 and 400 mg/Kg- every day, orally). Control mice received PBS at the same volume. At the end of study, macrophages isolated from peritoneal cavity of mice and the neutral red uptake, respiratory burst after challenge with tetra phorbol acetate and respiratory burst after challenge with opsonized yeast were evaluated in these population. Data were analyzed using the one-way ANOVA plus Turkey’s test. P values of less than 0.05 were considered statistically significant.

Results: The results of stimulation of macrophages with ester of phorbol showed a significant increase in respiratory burst of macrophages isolated from treatment groups compared to control mice (p<0.01). Nevertheless, when macrophages challenged with opsonized yeast, there was no significant difference between groups .Moreover, the results of neutral red uptake by macrophages didn’t show any significant difference between macrophages of control and treatment groups.

Conclusion: However, the hydroalcoholic extract of C. dactylon caused a significant decrease in the production of the potentially harmful free radical, but it could not interfere with the function of macrophages after challenge with a real infection.

Keywords: Cynodon dactylon, Macrophage, NMRI-mice.
Full-Text [PDF 164 kb]   (783 Downloads)    
Type of Study: Research | Subject: Immunology
Received: 2015/07/22 | Accepted: 2016/01/1 | Published: 2016/01/23
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Abtahi Froushani S. Effect of hydroalcholic extract of Cynodon dactylon the phagocytosis and respiratory burst of peritoneal macrophages of NMRI mice. Armaghane danesh. 2016; 20 (11) :985-995
URL: http://armaghanj.yums.ac.ir/article-1-974-en.html


Volume 20, Issue 11 (2-2016) Back to browse issues page
ارمغان دانش Armaghane danesh
Persian site map - English site map - Created in 0.06 seconds with 32 queries by YEKTAWEB 4162