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Antioxidant effect of hydroalcoholic extract of fenugreek leaves (L. Trigonella foenum-graecum) on changes in sex hormones in rat model of menopause
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Zahra Yazdan Bakhsh1   , Naktel Nektel Hossein Al Marzouk1 , Farangis Ghasemi2 |
1- Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran
2- .Department of Biology, Faculty of science, Zand Institute of Higher Education, Shiraz, Iran , ghassemi.fr@gmail.com |
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Abstract: (40 Views) |
Background and Objective: Menopause is accompanied by ovarian dysfunction and a decrease in sex hormones, which more or less affects the physical and mental health of women. It is important to provide treatment solutions with minimal side effects. Fenugreek (Trigonella foenum-graecum) may be a good option, because it contains phytoestrogenic and some antioxidant compounds.
Methods: After preparing hydroalcoholic extract of fenugreek leaves and determining the lethal dose, 48 adult female desert rats weighing 180-200 grams were selected and divided into 6 groups include control, untreated menopausal rats, and 2 treatment groups include menopausal model rats and two non-menopausal treatment groups. Menopause induction was performed by daily injection of 7mg/kg.bw of cisplatin for 5 days, and rats in both models were treated with doses of 250 and 500mg/kg.bw of fenugreek hydroalcoholic extract by daily gavage. At the end of the treatment period (24 days) and after taking blood from rat’s hearts, the serum concentration of gonadotropins and sex hormones was measured by radioimmunoassay method, and some oxidative stress factors were measured by ELISA method. The obtained data were analyzed with SPSS software and ANOVA test and compared with Tukey's test at a significance level of P≤0.05.
Results: Testosterone, progesterone, and estradiol hormones were significantly decreased in menopausal model rats compared to control, but luteinizing hormone and follicle stimulating hormone showed a significant increase, which was significantly modulated by the consumption of fenugreek leaf hydroalcoholic extract (especially at a dose of 500 mg/kg.bw). An increase in malondialdehyde enzyme was observed in menopause induced rats, and its compensation with the consumption of the mentioned extract, as well as an increase in total antioxidant capacity and superoxide dismutase, especially in non-menopausal rats compared to the control group.
Conclusion: Considering the modulating effect of the hydroalcoholic extract of fenugreek leaf on the examined hormones, especially in non-menopausal rats, and the reduction of malondialdehyde and the increase of antioxidant enzymes as a result of the consumption of the said extract even in menopausal groups, we can hope for the effect of this plant in reducing the adverse effects of menopause by emphasizing its antioxidant properties to some extent.Background and Objective: Menopause is accompanied by ovarian dysfunction and a decrease in sex hormones, which more or less affects the physical and mental health of women. It is important to provide treatment solutions with minimal side effects. Fenugreek (Trigonella foenum-graecum) may be a good option, because it contains phytoestrogenic and some antioxidant compounds.
Methods: After preparing hydroalcoholic extract of fenugreek leaves and determining the lethal dose, 48 adult female desert rats weighing 180-200 grams were selected and divided into 6 groups include control, untreated menopausal rats, and 2 treatment groups include menopausal model rats and two non-menopausal treatment groups. Menopause induction was performed by daily injection of 7mg/kg.bw of cisplatin for 5 days, and rats in both models were treated with doses of 250 and 500mg/kg.bw of fenugreek hydroalcoholic extract by daily gavage. At the end of the treatment period (24 days) and after taking blood from rat’s hearts, the serum concentration of gonadotropins and sex hormones was measured by radioimmunoassay method, and some oxidative stress factors were measured by ELISA method. The obtained data were analyzed with SPSS software and ANOVA test and compared with Tukey's test at a significance level of P≤0.05.
Results: Testosterone, progesterone, and estradiol hormones were significantly decreased in menopausal model rats compared to control, but luteinizing hormone and follicle stimulating hormone showed a significant increase, which was significantly modulated by the consumption of fenugreek leaf hydroalcoholic extract (especially at a dose of 500 mg/kg.bw). An increase in malondialdehyde enzyme was observed in menopause induced rats, and its compensation with the consumption of the mentioned extract, as well as an increase in total antioxidant capacity and superoxide dismutase, especially in non-menopausal rats compared to the control group.
Conclusion: Considering the modulating effect of the hydroalcoholic extract of fenugreek leaf on the examined hormones, especially in non-menopausal rats, and the reduction of malondialdehyde and the increase of antioxidant enzymes as a result of the consumption of the said extract even in menopausal groups, we can hope for the effect of this plant in reducing the adverse effects of menopause by emphasizing its antioxidant properties to some extent. |
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| Keywords: Estradiol, Gonadotropin, Fenugreek, Malondialdehyde, Menopause |
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Type of Study: Research |
Subject:
Physiology
Received: 2025/08/13 | Accepted: 2026/01/24 | Published: 2026/02/3
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