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The effect of geranium leaf essential oil Pelargonium graveolens on the expression of MMPs, IL-1β, NO, and PGE2 in collagenase-induced arthritis in rabbits
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Hossein Maghsoudi1   , Fereshteh Joulla2 |
1- Payam-e-Noor University Shar=e-Rey Branch , hossein_m2002@yahoo.com
2- Payam-e-Noor University Shar=e-Rey Branch |
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Abstract: (10 Views) |
Introduction: Chronic diseases, including osteoarthritis, are often debilitating due to their progressive course and affect the quality of life of the elderly. Among herbal products, geranium can be considered as a potential natural medicine due to the antioxidant properties of its leaf essence.
Aim of the research: In the present study, the physiological relationship of aromatic geranium leaf essential oil on cartilage protection in collagenase-induced osteoarthritis in New Zealand rabbits was determined.
Research method: After preparing the essential plant, the rabbits were kept for 1 month to adapt to the laboratory conditions. Collagenase was injected intra-articularly into the right knees of rabbits. 30 days after collagenase-induced osteoarthritis, rabbits were administered orally with distilled water (positive control), geranium essential oil (50, 100 mg/kg) and celecoxib (0.3 mL of 0.4%) once a day. became
Result: after the development of arthritis, the essential oil significantly reduced the stiffness of the articular cartilage in the affected rabbits. The expression of collagen type II, proteoglycan and aggrecan significantly increased in the rabbit knee joints of the group treated with essential oil. However, celecoxib had no effect on cartilage protection. The expression levels of ADAMTS-4, ADAMTS-5, MMP-1, MMP-3, and MMP-13 in the group treated with essential oil decreased in a dose-dependent manner. In contrast, the level of tissue inhibitory metalloproteinase increased in a dose-dependent manner. Proinflammatory cytokines involved in cartilage destruction, such as IL-1β, and inflammatory mediators containing PGE2 and NO were also inhibited in the essential oil-treated group.
These results show that the effect of geranium essential oil on the protection of affected cartilage is done by reducing the expression of inflammatory mediators and aggrecanases and MMPs.
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| Keywords: Osteoarthritis, Pelargonium graveolens, collagenase-induced osteoarthritis, celecoxib, metalloproteinase, aggrecanases |
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Type of Study: Research |
Subject:
Biochemistry
Received: 2025/01/25 | Accepted: 2025/08/25 | Published: 2025/08/26
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