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Evaluation of the Antibacterial Effect of Propolis Nanomaterials, Propolis Ethanolic Extract, Ciprofloxacin and Their Combination Against Pseudomonas aeruginosa
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T Archin1    , AG Onagh 2, AA Tehrani3 , S Kashipour4 |
1- Department of Microbiology, Urmia University, Urmia, Iran
2- Department of Microbiology, Urmia University, Urmia, Iran , a.ownagh@urmia.ac.ir
3- Department of Pathobiology, Urmia University, Urmia, Iran
4- Department of Nanochemistry, Nanotechnology Research Institute, Urmia University, Urmia, Iran |
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Abstract: (4909 Views) |
Background & aim: Pseudomonas aeruginosa is one of the most important opportunistic pathogens. Due to the increased use of antibiotics and the risk of their resistance, studies on the detection of natural antibacterial compounds have increased. Hence, the aim of the present study was to determine and evaluate the antibacterial effect of propolis nanomaterials, ethanosybenic anodic anodomic extracts of propolis, ciprofloxacin.
Methods: In the present experimental study, which was conducted at the University of Urmia in 2018, high-energy propolis nanoparticles were prepared using ultrasonic waves. Then the antimicrobial effect of propolis ethanolic extract, propolis nanoamulsion alone, ciprofloxacin alone and their combined combination (propolis + ciprofloxacin, propolis cyanide + ciprofloxacin nanoparticles) by microenvironmental microenvironmental microenvironmental and microbial methods Pseudomonas aeruginosa was diagnosed. Data were analyzed using one-way variance test. In this study, high-energy propellant nanoemulsion was prepared using ultrasound waves. Antibacterial effect of ethanolic extract of propolis, propolis nanoemulsion, Ciprofloxacin alone and their combinations (EEP+Ciprofloxacin and propolis nanoemultion + ciprofloxacin), were determined. For determination of MIC, MBC and FIC of each substance, Broth microdilution and Disk diffusion test were used. Data Statistical analysis was performed using SPSS software and one-way ANOVA test.
Results: First, 8,156 nm of propolis extract was prepared from propolis nanoparticles. The results of MIC and MBC experiments indicated that the minimum inhibitory concentration of alcoholic propolis extract extract and propolis nanomaterials against Pseudomonas aeruginosa was 1000 and 46.88 micrograms per milliliter, respectively. Moreover, the evaluation of the combined effect of propolis extract and propolis nanomaterials with ciprofloxacin is 9.46 2 2.44, 7.3 22 1.32 μg / ml, respectively. Furthermore, platelets containing combined propolis nanoamulsion dissociation disks with ciprofloxacin with concentrations of 1.32 7 3.7, micrograms per milliliter of halo growth, respectively, caused a growth of 15 mm in diameter, while single-use nanograms per 6 milli6 milligrams of propolis The growth control halo was 15 mm larger.
Conclusion: In the present study, for the first time, nanoamulsion was prepared from the ethanolic extract of propolis, which alone and in combination with ciprofloxacin has the effects of inhibiting growth inhibition and high elongation on Pseudomonas aeruginosa. It had a synergistic effect and as a result, the dose of each of the compounds and the time required to kill the bacterium was reduced by comparing the use of each of them alone.
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| Keywords: Pseudomonas aeruginosa, Ciprofloxacin, Propolis Nanoemulsion, Ethanolic extract of propolis |
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Full-Text [PDF 2276 kb]
(740 Downloads)
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Type of Study: Research |
Subject:
Microbiology
Received: 2019/01/11 | Accepted: 2019/06/9 | Published: 2020/03/4
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