|
The Effects of LeukemiaInhibitory Factor onDevelopment of MousePreimplantation Embryo
|
I Amiri   , M Parvini , A Amini , R Mahmodi 1 |
| 1- , rmahmoudi40@yahoo.com |
|
|
Abstract: (3943 Views) |
| Introduction and Objective: Recent studies have demonstrated
that mammalian preimplantation embryos are exposed to a mixture
of many different growth factors and cytokines, expressed by the
follicles, oviducts and endometrium. Receptors for many of these
growth factors have also been shown to be expressed by
preimplantation embryos. In vitro culture of human and animal's
embryos in conventional media lacking growth factors can result in
suboptimal growth and a variety of short-term and long-term
developmental abnormalities. One of these factors is Leukemia
Inhibitory Factor (LIF). The aim of this study was to evaluate the
effects of LIF on the mouse preimplantation embryo development.
Materials & Methods: Six to eight weeks old NMRI mice were
superovulated by injection of 10IU PMSG and 10IU HCG 48h later.
The mated mice were killed 48 hours after HCG injection, oviducts
were flushed and two-cell embryos were collected and divided
randomly to two groups (control and treatment). Control medium
was HTF and treatment medium was HTF+1000u/ml LIF. In each
group the embryos were cultured in an incubator at 37°C with 5%
CO2 for 72h.The state of embryo development was evaluated in 12
hours interval using inverted microscope.
Results: There was not any significant difference in the rate of
morolla and blastocyst formation after 36 hours. In comparing
hatching rates, 60 and 72 hours after culture, there were significant
difference between control and treatment groups (p< 0.008).
Conclusion: LIF doesn’t provide obvious stimulation in the early
mouse embryo development until morolla stage. However, it has
positive effects on preimplantation blastocyst growth, differentiation
and hatching. |
|
| |
|
|
Full-Text [PDF 675 kb]
(1234 Downloads)
|
Type of Study: Research |
Subject:
Special
Received: 2015/06/1 | Accepted: 2015/06/1 | Published: 2015/06/1
|
|
|
|
|
|
|
| Send email to the article author |
|
|
|
| Add your comments about this article |
|
|
|
