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Molecular Identification of SHV,TEM, CTX-M β lactamases Genes and Antibiotics Resistance Pattern of k.pneumoniae Isolates Collected from ICU Patients of Namazi Hospital, Shiraz, Iran
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T Archin   , E Afzalian 1, M Kargar , Y Ghasemi |
| 1- , es.afzalian@gmail.com |
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Abstract: (5122 Views) |
| Abstract
Background and aim: β-lactamase enzymes producing bacteria ESBL have spread widely throughout the world. The production of enzymes induces bacterial resistance to a wide range of antibiotics which is leading to the limitation of infection control and correct treatment. The aim of the present study was to investigate patterns of antibiotic susceptibility to antibiotics and the presence of β-lactamase genes SHV, TEM, CTX-M, in Klebsiella pneumoniae isolates from clinical specimens of intensive care.
Methods: Susceptibility of isolated bacteria against 10 antibiotics was determined by agar disk diffusion method according to the CLSI guidelines. The strains (DDST) were examined for the presence of the spectrum β-lactamase enzymes. Using E-test, the minimum inhibitory concentration (MIC) of the antibiotic was determined to cefotaxime. Moreover, SHV, TEM, CTX-M genes were identified by, Multiplex PCR method, and some of them were sequenced.
Results: The antibiotic resistance against 10 antibiotics was determined. The highest percentage of isolates was resistant to ampicillin (100%) and sensitivity to imipenem was 1.66%). In this study, the majority of strains produced ESBL (60%). TEM gene in 34.38% and all three genes (TEM and SHV and CTX) at 33.13% of isolates were observed.
Conclusion: The present study showed that the K. pneumoniae producing ESBL in patients in ICU are common. Therefore, the use of procedures and policies for infection control in hospitals and especially ICU is necessary.
Key words: Klebsiella pneumoniae, ESBL, Multiplex PCR, antibiotic sensitivity |
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| Keywords: Key words: Klebsiella pneumoniae, ESBL, Multiplex PCR, antibiotic sensitivity |
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Full-Text [PDF 1219 kb]
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Type of Study: Research |
Subject:
Special
Received: 2015/05/6 | Accepted: 2015/05/6 | Published: 2015/05/6
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