1 1728-6506 Yasuj University Of Medical Sciences 540 Special Effects of Topiramate in the Prevention of Drug Resistant Migraine Headache Khani H Abedini M Zarvani A Azadmarzabadi A Roshanbakhash A Roshanbakhash R Ghorbani A 1 4 2009 14 1 1 11 17 05 2015 17 05 2015 ABSTRACT Introduction & objective: Migraine is the most common cause of headache. The aim of the present study was to evaluate the effects of topiramate (TPM) in the prevention of drug-resistant migraine headache. Materials & Methods: This is a double-blind clinical trial conducted on 70 patients between ages 15 to 45 years referred to the Bu Ali Sina Hospital in Sari with a history of migraine attacks based on International Headache Society criteria for a period of more than one years with a minimum incidence of 1 to 6 attacks per month. The drug rate performance was assessed by response rate to treatment, mean changes in the number and severity of migraine attacks compared with the placebo group for 3 months. Collected data were analyzed using analysis of variance (ANOVA), Newman-Keuls and Spearman’s Coefficient Rank Correlation as the post hoc tests. GRAPHPAD software was used for analysis of the data. Results: 66 of 70 patients completed the study. The mean age of the patients was 30.33±7.9 years. A significant reduction in the severity and frequency of migraine attacks was seen in all months (P < 0.0001) for topiramate treated group in compare to placebo group. Responder rate for patients treated with TPM was significantly higher than placebo group (63.6%, P<0.0001) in the 3rd month of the treatment Side effects of treatment were transient and well tolerated. Conclusion: Low dose of TPM showed significant efficacy in prevention of migraine attacks within the first, second, and third month of treatment. Low dose of TPM seems to be a good therapeutic option for the patients with refractory migraine.
541 Special The Effect of Radioactive Lantern Mantle Powder and Bentonite-Zeoloite Minerals on the Volume of Blood Loss, Bleeding and Clotting Time Atefi M Mortazavi SMJ Roshan-shomal P Raadpey N Motazedi S Darvish M 1 4 2009 14 1 12 23 17 05 2015 17 05 2015 ABSTRACT Introduction & Objective: Over the past decade the US army has widely studied new technologies for stopping sever hemorrhages and has introduced an effective Zeolite based hemostatic agent. On the other hand, Mortazavi and his colleagues previously reported the bio-stimulatory effects of the topical application of radioactive lantern mantle powder on wound healing. Their subsequent studies showed significant changes in some histological parameters concerning healing. In this light, here the bio-stimulatory effect of burned radioactive lantern mantles powder as well as two minerals bentonite and zeolite are presented. Materials & Methods: This experimental study was conducted in the center for radiological studies, Shiraz University of Medical Sciences in 2008. Fifty male Wistar rats were divided randomly into 5 groups of 10 animals each. Following anesthesia, animals’ tails were cut at a thickness of 5 mm by using a surgical scissor. No intervention was made on the animals of the 1st group. The 2nd to 4th group received topical non-radioactive lantern mantle powder, radioactive lantern mantle powder, Bentonite mineral or a mixture of Bentonite-Zeoliteat minerals respectively. After treatment with above mentioned agents, the volume of blood loss was measured using a scaled test-tube. The bleeding time and clotting time were also measured using a chronometer. SPSS software was used for statistical analysis. ANOVA was used for comparing the means of each parameter in the 5 groups. Results: The the volume of blood loss, bleeding and clotting times in control animals were 4.39±1.92 cc, 112.10±39.60 sec and 94.9±54.26 sec, respectively. In the 5th group in which the animals were treated with a mixture of Bentonite-Zeoliteat minerals, the volume of blood loss, bleeding and clotting times were 1.31±0.60 cc, 34.50±4.65 sec and 24.2±4.61 sec, respectively. Conclusion: This is the 1st investigation that studied the alterations of bleeding and clotting times following the use of lantern mantle powder as well as bentonite or the mixture of bentonite-zeolite minerals. The results obtained in this study clearly show the significant effect of the volume of blood loss as well as the bleeding time following the topical use of the mixture of bentonite-zeolite minerals. The radioactive and non-radioactive lantern mantle and the bentonite mineral alone had considerable effects but overally the magnitude of the effect was less than that of the mixture of bentonite-zeolite minerals. 542 Special The Effects of Hesperidin, on Cardiac Dysfunction of Experimental Type-I Diabetic Rats Kouhpayeh A Mirkhani H Nekooeian AA Saint D 1 4 2009 14 1 25 35 17 05 2015 17 05 2015 ABSTRACT: Introduction & Objective: Flavonoids are polyphenolic compounds, which are considered as antioxidants due to their ability to scavenge free radicals and inhibit enzymes in oxygen-reduction pathways. Various studies have shown that these products reduce the cardiovascular disease mortalities. Heart failure is one the main cause of mortality in diabetic patients. It is believed that diabetes has deleterious cardiomyopathic effects, which would lead to heart failure. Several evidences indicate that oxidative stress is an important factor in the pathogenesis of diabetic complications, including cardiomyopathy. The objective of the present study was to examine the effects of hesperidin on cardiac function parameters in experimental diabetes mellitus type 1 (DM1). Material & Methods: Diabetes mellitus was induced in rats by single intraperitoneal injections of streptozotocin (60mg/kg). Diabetic rats were given oral Hesperidin (500 mg/kg) for two months. Afterwards, the animals' hearts were used to study left ventricular systolic pressure (LVSP), rate of rise (+dP/ dT) and rate of decrease (-dP/ dT) of left ventricular pressure, using Langendorff isolated heart apparatus. Results: Diabetes significantly reduced the LVSP, +dP/ dT and -dP/ dT compared to the control group(p<0.05). Hesperidin significantly improved all measured parameters in diabetic animals(p<0.05). Conclusion: These results show that hesperidin can improve diabetic cardiomyopathy in experimental diabetes mellitus. 570 Special In vitro Effect of Chamomile, Clove and Garlic Extract on Non-Enzymatic Glycosylation of Albumin Sirati Sabet M Geibi N Abbasi E Khabbaz F 1 4 2009 14 1 37 45 26 05 2015 26 05 2015 ABSTRACT: Introduction & Objective: Diabetes mellitus is a common disease in human societies. Proteins non-enzymatic glycation has deleterious effects on the structure and function of proteins. Non-enzymatic glycosylation of proteins can be involved in the pathogenesis of diabetic complications such as retinopathy and nephropathy. One way of preventing this reaction is the use of medicinal plants. Chamomile, clove and garlic are herbs which their alkaloid compounds have antioxidant property. The aim of this study was to evaluate the effect of chamomile, clove and garlic extract on non-enzymatic glycosylation of albumin. Materials & Methods: In this experimental study, hydroalcoholic extract of chamomile, clove and garlic was prepared. In vitro albumin glycosylation reaction was carried out in presence of different concentrations (0.01, 0.05 and 0.1 g/L) of chamomile, clove and garlic extracts and level of glycosylated albumin was measured. Non-enzymatic glycosylation of albumin was determined by nitrobluetetrazuliom method. Absorbance changes were measured in 530 nm by Eppendorf spectrophotometer. Results: In this study, chamomile extract in 0.01, 0.05 and 0.1 g/L of its concentrations had respectively 9%, 17% and 26%, inhibitory effect on albumin glycosylation. Albumin glycosylation reaction was inhibited 23% by 0.01 g/L concentration of clove and garlic extracts and the level of inhibition of albumin glycosylation decreased by increasing of extracts concentration. Chamomile extract with 0.1 g/L concentration has the most effect among the studied extracts. Conclusion: In this study, chamomile extract inhibited the in vitro albumin glycosylation reaction. Clove and garlic extracts showed inhibitory effect in low concentration but this effect decreased with increasing of the extract concentration. 571 Special Twenty four Hours Circadian Cortisol Profile in Shift Work Nurses Kakooei H Zamanian Ardakani Z Karimian M Ayttollahi T 1 4 2009 14 1 47 56 26 05 2015 26 05 2015 ABSTRACT: Introduction & Objective: It is well established that adrenal hormone cortisol has long been used in human psychobiological studies as a biological marker of circadian rhythm, stress, and anxiety. There is little information, however, about the interrelationships of night work, plasma cortisol levels in females’ nurses of the developing countries. The aim of the present study was to compare circadian cortisol profile between two groups of nurses with shift and permanent work in a Hospital affiliated to Shiraz University of Medical Sciences, Iran, between January 2006 to September 2008. Materials & Methods: In this experimental study, we examined the reproducibility of plasma cortisol levels during one month period in 34 shift work nurses and 10 permanent day shift works. We evaluated potential associations between night work and hormone levels, using current and shift work information for the health care shift workers. Results: The results clearly revealed that shift work significantly increased nighttime cortisol levels (34.93 μg/dl) during night shift and decreased the peak levels of cortisol in the morning (39.03 μg/dl). During the work shift the plasma cortisol presented a decreased concentration in the morning and increasing levels (44.93 μg/dl) by the end of the evening. In contrast , the permanent day work nurses, who had never worked at night shifts, showed a lowest secretion of cortisol during the first half of night time sleep (10.02 μg/dl ), and a peak value of the cortisol after awakening at 07:00 h ( 59.75 μg/ml). We also observed a significant association between night work and increased levels of cortisol over the remaining of the day. Conclusion: It can be concluded that nurses with night shift work have an abnormal cortisol secretion profile. Shift work nurses on rotating night shifts seem to experience changes in hormones levels that may be associated with increased of circadian disorders risk observed among night – shift workers. 572 Special Comparison of Marking Changes in Neural Cells Detection Protocol on Superior Laryngeal Nerve in Dogs Ghanbari Hassanabad A Mesbah Ardekani SH Namavar MR Khademi B Karbalaydoust S 1 4 2009 14 1 57 65 26 05 2015 26 05 2015 ABESTRACT Introduction & Objective: Neuron tracing is a novel method that is used for detecting communications between cells, tracing nervous pathways, and recognizing the nuclei relating to different pathways. One of the most common tracing methods is putting cutting end of nerve inside the tracer solution. This method is so sensitive that improper insertion of the nerve inside a solution or histological preparation, would lead not to observing the tracers. We evaluated methods for observing tracers. Material & Methods: 10 adult dogs were assigned into two groups. In the first group, the superior laryngeal nerve was cut and its proximal end, after washing and des-heating, was put inside the horse radish peroxidase (HRP) solution. In the second group, after washing the proximal cutting end of superior laryngeal nerve, we separated tiny vessels supplying the nerve to prevent hemorrhage. Then, by using the needle (gauge 22), 2-3 distal end of nerve sheath was precisely removed and nerve fibers were separated and kept inside the HRP solution. In both groups, nerve end was kept inside the HRP solution for 2 hours. Three to five days later, after perfusion and fixation their head and neck, their pons, medulla oblongata, three upper spinal segments and vagal ganglions were removed, sectioned and prepared histochemically and stained with thionin. Results: The number of labeled cells in the first group was less (2-4 cells per section) than the second group (4-8 cells). This difference was statistically significant (P < 0.05). Conclusion: This study showed that separating nerve fibers and fixation of nerve end inside the solution increased the absorption of tracer. 573 Special The Effect of Cinnamon (Bark) Extract on Male Reproductive Physiology in Mice Modaresi M Messripour M Rajaei R 1 4 2009 14 1 67 77 26 05 2015 26 05 2015 ABSTRACT: Introduction and Objective: Cinnamon is a plant with the scientific name Cinnamomum zeylanicum that belongs to the Lauraceae family. This plant has many therapeutic effects one of them is the increasing of sexual desire. This study was conducted to find out the effects of Cinnamon extracts on reproductive physiology of male laboratory mice. Materials and Methods: Animals were assigned in six groups, each consisted of eight mice. The mice groups were experimental groups (1, 2, 3, 4) and two control and placebo groups. All animals were kept in same condition. Different doses of Cinnamon hydro-ethanolic extract (50,100,200 and 400mg/kg/2day) were injected, intraperitonealy, to animals for 20 days while the control group received normal saline plus ethanol. The most important parameters in this study were included: variation in testicles weight, probabe histological changes in testes, changes in number of sexual cells and density of LH, FSH and testosterone in blood of the subjects. Results: The results indicated that cinnamon can significantly increase the level of LH and FSH in doses of 200, 400 mg/kg. The density of testosterone increased in dose of 50, 100 mg/kg also the number of sperms and primary spermatocytes raise in 100, 200 and 400 cinnamon extract while no significant changes were observed in weight of testicles and also in histological findings. Conclusion: The findings of this research indicated the positive effects of cinnamon extract on male reproductive system and hormonal changes in pituitary-gonad axis because sperm count and secretion of FSH hormone had a meaningful significance in dose of 200 and400 mg/kg of the cinnamon extract. 574 Special The Impact of the Mothers' Nutritional Education on 12-30 Months Toddlers Food Pattern Gashmard R Kashani nia Z Sajedi F Rahgozar M 1 4 2009 14 1 79 89 26 05 2015 26 05 2015 ABSTRACT: Introduction & Objective: Considering the importance of nutrition in early years and mother’s prominent role ,the objective of the present study was to evaluate the impact of nutritional education to mothers on 12-30 months toddlers food pattern. Materials & Methods: In this quasi-exprimental study subjects were consisted of 242 mothers with their 12-30 months toddlers who were referring to health-care centers in Tehran, Iran, in 2006. After defining the number of the samples, allocation of each health-care center was done by PPS1 method and then sequentially distributed into two groups, intervention and control. Data collection was done in two stages: at the beginning of the study and one month after intervention by three tools 1)questionnaire 2)scale 3)tape meter. During three months, mothers who participated in nutrition education program completed the FFQ. The researcher gave adequate comments to intervention group by face to face teaching. Data analysing was done by chi-squre, paired t.test & analysis of variance of repeated measures. Results: Before intervention, condition of food score was 6.859 and Z score was 2.789. After intervention, the conditionof food score was 7.983 and Z score was found to be 1.722. Before the intervention, in both groups, meat, fat, and sugar consumption was higher than normal ranges. After the intervention, fruit and vegetable consumption was increased. Before intervention, the condition of food questionnaire score was in middle range in two groups while the condition in intervention group was increased after intervention. Conclusion: Z score before intervention in two groups shows overwieght, but after the intervention lossing of wieght was seen. 576 Special Hypoglycemic Effects of Achillea Wilhelmsii in Normal and Streptozotocin Induced Diabetic Rats Sadeghi H Radmanesh E Akbartabar Turi M Mohammadi R Nazem H 1 4 2009 14 1 92 99 26 05 2015 26 05 2015 ABSTRACT Introduction & Objective: Diabetes mellitus is a syndrome, initially characterized by a loss of glucose homeostasis resulting from defects in Insulin secretion, insulin action both is resulting in impaired metabolism of glucose and other energy yielding fuels as lipids and protein. Several medicinal herbs have been described with hypoglycemic effects. These include: Allium Sativum, Trigonella Foenum, Marus nigra, Ocimum Sanctum, and Astragalus Ovinus. The main purpose of the present study was to determine the effect of Achillea Wilhelmsii C. Koch on blood glucose levels of diabetic rats induced by stereptozotocine (STZ). Materials & Methods: In this experimental research, forty-eight male Wistar rats were divided into two groups: non-diabetic (normal) and STZ-induced diabetic mice. Each group was further divided into four groups: control (induced by normal saline) and treatment received 100, 200.and 300 mg/kg aqueous- alcoholic extract of Achillea Wilhelmsii C. Koch daily for one month. The blood glucose level was measured and Data were analyzed by t-test and ANOVA. Results: At the end of first month, significant decrease was observed in blood glucose level in diabetic rats which received 100 mg/kg (p<0/001), 200mg/kg(p<0/01), 300mg/kg (p<0/001) of aqueous alcoholic extract of Achillea Wilhelmsii C. Koch in comparison with control groups. The extract had not have any significant effects on the blood glucose level of normal groups except in those which received 300mg/kg of the extract. Conclusion: The results of this study showed that aqueous- alcoholic extract of Achillea Wilhelmsii C. Koch have a significant effect on reducing the blood glucose level of diabetic rats. 578 Special The effect of Benson Relaxation Technique on Anxiety Level and Qquality of Life of Patients with Irritable Bowel Syndrome Hoseini M نیک‌روز L Poursamad A Taghavi A Rajaeefard A 1 4 2009 14 1 101 111 26 05 2015 26 05 2015 ABSTRACT: Introduction & Objective : Irritable bowel syndrom (IBS) is the most common functional bowel disorder which both the bowel and the brain are involved in this syndrom. Combined medical - relaxational therapy has been suggestd to decrease anxiety and relieve symptoms, make better feeling of self health and improve the quality of life in these patients.This study was designed to determine the effects of Benson relaxation technique on anxiety level and quality of life of patients with irritable bowel syndrome. Materials & Methods: In a clinical trial, 30 patients with irritable bowel syndrom were selected and randomly placed into two groups: case and control.The control group was treated by only usual medical therapy but the case group was treated by combined medical - relaxational therapy. The two groups continued therapy for three months. The anxiety of patients was measured by Spielberger test [State; Trait Anxiety Inventory (STAI)]. The quality of life of patients with regard to irritable bowel syndrome, was measured by BS-QOI with subscales 8, before intervention,1 week and 3 months after intervention in both groups. The collected data were analyzed by descriptive and Mann-whitney, repeated measurement tast and chi-square statistical methods. Results: State and trait anxiety mean of the case and control groups two weeks before and one week after intervention didn’t show any significant differences. But after three months the results showed a significant difference between the two groups(p<0.05). There wasn’t significant difference in scales of quality of life before intervention. One week after the intervention, differences of both group in four scales of dysphoria, health worry, social reaction and relationship were meaningful and the function of case group was more undesirable than that of the control group.Three months after completing the process, there wasn’t a meaningful increase in sex function in case group in comparison with the control group but there was a significant recovery in the case group in other performance scales (p<0.001). The quality of life of patients in control group compared to case group decreased or did not change significantly after 3 months. Conclusion: Performing Benson relaxation therapy is effective in reducing anxiety level coinciding with improvement quality 0f life therefore relaxation therapy can be recommended as an effective care program. 580 Special Relationship of Mother's Depression and Child Enuresis Arman S Karbasi Amel A Hydari A 1 4 2009 14 1 114 120 26 05 2015 26 05 2015 ABSTRACT: Introduction & objective: Functional enuresis, as a common psychiatric problem in children, can cause depression in mothers and also low quality of life. Considering the fact that the family has an important role in mental health of their child, it can be expected that the mother's depression can cause enuresis in her child. Up to now there had been no study about the relationship of mother's depression and child enuresis. The present study is designed with the aim of evaluating this association. Materials & Methods: In this descriptive- analytic cross sectional case-control study, 48 girls and 48 boys with the diagnosis of enuresis based on criteria of DSM-IV-TR that were admitted to the clinics of Isfahan University of Medical Sciences, from 2004-2005 were selected as the case group and 96 children without enuresis (48 girls and 48 boys) as the control group. The average age of both groups was 9 years. Then the mothers of the children of both groups answered to the Beck Depression Inventory (BDI). Results: The prevalence of depression in case group was significantly higher than the control group (P<0.001). The prevalence of depression in mothers with enuretic girls was significantly more than the enuretic boys (P<0.04). But the relative prevalence of depression of non enuretic girl's mothers didn't show significant diference from the non enuretic boy's mothers. Conclusion: Enuresis in children, especially in girls, is accompanied by increased prevalence of mother depression as compared with the control group. So in treatment of enuresis, especially in girls, attention to the treatment of mother’s depression and her support is crucial. 581 Special Birth Injuries and Related Risk Factors in Neonates Born in Emam Sajjad Hospital in Yasuj in 2005 to 2006 Rezaie M Ghafarian Shirazi HR Balaghi Einanloo K Hashemi H 1 4 2009 14 1 122 129 26 05 2015 26 05 2015 ABSTRACT Introduction & Objective: The first days of life has the highest risk in everyone’s life and the mortality rate in these days is equal to the mortality rate of the whole life. One of the causes of mortality in these days is birth injuries. Determining the type and incidence of birth injuries and their risk factors can be useful in reducing the mortality rate in neonates. Materials & Methods: In this descriptive analytical study, 2005 neonates who were born in Imam Sajad Hospital in Yasouj/ Iran, between the years 2005 to 2006 were studied. The rate of birth injuries and their risk factors were evaluated. Collected data were analysed by SPSS software using chi-squar test. Results: Out of 2005 neonates, 70.4% born by NVD and 29.6% by cesarian section. The study showed that 10.8% of NVD and 3.7% of cesarian section neonates had birth injuries. Two hundered and four of these injuries which occured in 175 of neonates were incude: capute succedaneom (36.7%), sub conjectival hemorrhage (27.9%), cephal hematoma (10.2%), facial erythema and abrasion (8.8%), facial echymosis (6.3%), hypoxic-ischemic encephalopathy (3.4%), brachial plexus palsy (3.4%), clavicular fractures (1.4%), facial nerve palsy (0.4%) and other skin lesions (0.9%). Risk factors were included NVD (difficult vaginal delivery), high gestation age, and low Apgar score at first minute of life, shoulder dystocia, vacuum and birth at night. Conclusion: The present study revealed that the incidence of birth injuries in this area is high. Considering the serious complications of birth injuries and hypoxic-ischemic encephalopathy with no treatment for some cases, it seems that using the preventing methods to reduce the prevalence of birth injuries is nessesary 582 Special An unusual Case of Transitional Cell Carcinoma of Renal Pelvis Presenting with Brain Metastases Razzaghi MR Nicknam SH Mohammad hosseini M Rezaei AR Javanmard B Rezaei I 1 4 2009 14 1 132 138 26 05 2015 26 05 2015 ABSTRACT: Introduction & Objective: Transitional cell carcinoma of renal pelvis presenting with brain metastases is a very rare case which should be diagnosed and treated in order to prevent further damages. Case: We report a rare case, who had presented with a constellation of neurological symptoms (due to multiple brain metastases), but without any urological symptoms. During evaluation of patient, we found transitional cell carcinoma (TCC) of left renal pelvis, for which palliative radical nephroureterectomy was performed . Conclusion: Although transitional cell carcinoma of renal pelvis presenting with brain metastases is a very rare case, but the patient was managed with gamma knife stereotactic radiosurgery for the metastatic lesions. Afterward he received four cycles of paclitaxel and carboplatin chemotherapy. The patient is alive with stable disease at 32- months’ follow-up.