1 1728-6506 Yasuj University Of Medical Sciences 3127 Biochemistry Toxicity and Apoptotic Effect of Nanoliposomes Containing Curcumin and miR-34a on Colorectal Cancer Cells (HT-29) and Breast Cancer(MCF-7) Nikseresht M b Ghasemi Chaleshtari M c Tajali Ardakani M d Abidi H e Mahmoudi R f Bardania H g b Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran c Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran d Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran e Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran f Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran g Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran 1 12 2021 26 6 867 883 23 07 2021 11 12 2021 Background & aim: Colorectal and breast cancer are the leading causes of death in the world. The aim of the present study was to determine the toxicity and apoptosis effect of nanoliposomes containing curcumin and miR-34a on colorectal cancer cells (HT-29) and breast cancer (MCF-7).   Methods: In the present experimental study conducted in 2018, nanoliposomes containing two compounds of curcumin and miR-34a were prepared and characterized using dynamic light scattering analysis and transmission electron microscopy. To evaluate the effect of nanoliposomes, HT-29 and MCF-7 cancer cell lines were cultured in DMEM and RPMI 1640 with bovine fetal serum and antibiotics, respectively. Cells were treated with nanoliposomes containing curcumin, miR-34a and nanoliposomes containing curcumin and miR-34a, and their viability was determined by MTT assay. Also, the percentage of cell death (apoptosis) was measured using flow cytometry. Data were analyzed using SPSS software and one-way ANOVA.   Results: The results indicated that co-administration of miR-34a and curcumin increased their anticancer effect. In addition, curlumin-containing nanoliposomes can kill cancer cells in a time- and dose-dependent manner. On the other hand, nanoliposomes containing miR34a killed cancer cells at a concentration of 5 nM after 48 hours. The results of MTT assay and apoptosis analysis using flow cytometry showed that simultaneous transfer of curcumin and miR-34a enhanced their effect on both cell lines (HT-29) and (MCF-7) after 48 hours.   Conclusion: The present results revealed that the loading of miR-34a and curcumin in nanoliposomes significantly increases their effects. In addition, co-administration of these drugs synergistically enhances their anti-cancer effects. The findings of the present study exhibited that the concomitant use of curcumin and miR-34a via nanoliposomes could be considered as a new combination therapy for cancer treatment.    
2856 Physiology Evaluation of the Effects of Thymol on Non-Alcoholic Fatty Liver Caused by Chronic Immobility Stress in Rats Miri S h Komeili GH i Niazi AA j Shahraki MR k h Department of Physiology, Zahedan University of Medical Sciences, Zahedan, Iran i Department of Physiology, Zahedan University of Medical Sciences, Zahedan, Iran j Departments of Pathology, Zahedan University of Medical Sciences, Zahedan, Iran k Department of Physiology, Zahedan University of Medical Sciences, Zahedan, Iran 1 12 2021 26 6 884 898 11 07 2020 08 11 2021 Background & aim: Thymol is a phenolic monoterpene that has been reported its antioxidant and anti-inflammatory properties in previous studies and appears to have a protective role for the liver against damage caused by free radicals. Therefore, the aim of the present study was to determine the effects of Thymol on non-alcoholic fatty liver caused by chronic immobility stress in rats.   Methods: The present experimental study was conducted in 2020. 24 Wistar rats were included in the study and divided into 3 equal groups; Control sham, negative control and treatment (Thymol recipient at a dose of 100 mg/kg). After 60 days, the mice were anesthetized with ketamine and xylazine and blood samples were taken from the heart and the whole liver was removed for histological examination. Inflammatory factors including IL-6 and TNF-α, antioxidant enzymes including; GPx, SOD and catalase, fat profiles including LDL, HDL, triglyceride and total cholesterol as well as malondialdehyde (MDA) were examined. The collected data were analyzed using one-way analysis of variance and Turkey’s post hoc test.   Results: The results indicated that in the fatty liver group, the amount of antioxidant enzymes decreased and fat factors and the amount of MDA increased. While treatment with Thymol was able to bring these factors closer to normal. Pathological results exposed that Thymol can reduce the amount of damage caused to non-alcoholic fatty liver by reducing cell necrosis.   Conclusion: The present study revealed that Thymol with antioxidant properties can improve        non-alcoholic fatty liver function due to chronic immobility stress by reducing inflammatory markers, increasing antioxidant indices and reducing cell necrosis.      2325 Special Effects of Two Corrective Protocols on Trunk Muscles in Females with Hyperlordosis; Williams vs McKenzie’s Protocol Ghoreyshi Nejad SH l Kheyrabadi S m Jashanzadeh O n avid M o Fatemi R p l Department of Sport Physiology, Shahid Chamran University, Ahvaz, Iran m Department of Physical Education, Islamic Azad University, Shiraz Branch, Shiraz, Iran n Department of Physical Education, Islamic Azad University, Shiraz Branch, Shiraz, Iran o Department of Physical Education, Islamic Azad University, Dehdasht Branch, Dehdasht, Iran p Department of Physical Education, Islamic Azad University, Dehdasht Branch, Dehdasht, Iran 1 12 2021 26 6 899 914 22 10 2020 08 11 2021 Background & aim: One of the most common causes of trunk deviations is a change in the curvature of the spine in the lumbar region. Physiotherapy is one of the most common treatments for hyperlordosis and low back pain. In the field of corrective exercises, the comparison of the findings of Williams and McKinsey's research is small and contradictory. The aim of the present study was to determine and compare the effects of two types of corrective exercise program on lumbar muscle strength in girls with hyperlordosis.   Methods: In the present quasi-experimental study conducted in 2019, 30 females with lumbar lordosis beyond normal degrees (Hyperlordotic) in lumbar curve were selected as the study sample and were randomly divided into Williams and McKenzie’s exercise groups. Training protocols were done for eight weeks, three times a week. The lumbar lordosis was measured using a flexible ruler, isometric strength of trunk and hip muscles were measured by using digital dynamometer and precieved pain was measured using Copeck Pain Questionnaire. Data was analyzed using independent and paired t-testes.   Results: The results indicated that both types of exercises lead to a significant increase in the strength of the lumbar, extensor and pelvic flexor muscles as well as the strength of the abdominal muscles. Exercises in both groups correspondingly significantly reduced the subjects' low back pain (p <0.001), nonetheless, no significant difference was observed between the results of the two types of exercises in any of the study variables (p> 0.05).   Conclusions: The results indicated that the administration of Williams and Mackenzie corrective movements can lead to a significant improvement in lumbar arch angle and isometric strength of trunk muscles; however, in terms of effectiveness, no significant difference was observed between the two training methods.       3126 Special A Molecular Docking Study of Phytoestrogenic Compounds from Hypericum perforatum L. and Anethum graveolens L. with Two Isoforms of Estrogen Receptor Mansourian M Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran 1 12 2021 26 6 915 940 22 07 2021 23 08 2021 Background & aim: Estrogens play a key role in the growth, development and maintenance of a wide range of tissues. Physiological responses to estrogen in specific tissues are mediated by at least two isoforms of the estrogen receptor alpha (ERα) and beta (ERβ). Currently, medicinal plants are widely used in the treatment and control of diseases due to their lower cost and fewer side effects than synthetic drugs, including Hypericum perforatum L. and Anethum graveolens L. Hypericum perforatum L. is effective in premenstrual syndrome, and Anethum graveolens L. as a regulator, effective in sex hormones and dysmenorrhea. The main purpose of the present study was to compare the molecular interaction of phytoestrogenic compounds of the studied plants with these two isoforms of estrogen receptor through molecular docking.   Methods: This molecular docking study was performed in 2021. The Molecular docking method was used to calculate the molecular interaction with the aim of additional investigation of the possible hormonal effects of phytoestrogenic compounds in the studied plants. Evaluation of theoretical binding site of flavonoid phytoestrogenic compounds fisetin, kaempferol, quercetin, myricetin, tetramethyl stilbene, trans-Anethole, limonene, resveratrol, and genistein as an agonist or antagonist was performed by ERα and ERβ. The amino acid residues involved in the interaction, the free binding energy and the binding constant were determined. The collected data were analyzed using different software and compared with the results of related articles.   Results: By examining the binding energy and different conformations of phytoestrogenic compounds into ERs, fisetin, kaempferol, quercetin, myricetin and resveratrol were selected as the best potential ligands. The results confirm that these compounds were quite sterically compatible with the location at the active site occupied by the 17β-estradiol endogenous agonist as full agonist and partial agonist genistein. The results of molecular docking provided evidence of the binding of phytoestrogenic compounds to ERα and ERβ with a bond-free energy of -5.26 to -8.47 kcal/mol and a binding constant of 131.62 µM to 614.61 nM and their interaction with key residues of active site including Glu353, Arg394 and His524 of ERα and Glu305, Arg346 and His475 of ERβ as partial agonist.   Conclusion: The interaction of the best phytoestrogenic compounds with the key amino acids of active site ERs such as the partial agonist of genistein was desirable. Valuable results of molecular docking in identifying the most important phytoestrogenic compounds can be considered as a starting point for optimization in the rational design of drugs in the treatment of estrogen-sensitive diseases.     3151 General Early Detection of MS in fMRI Images of the Brain Using Deep Learning Techniques Vahidian E Fatehi Dindarloo MH Jamali J Taghizadeh M Department of Medical Engineering, Kazerun Branch, Islamic Azad University, Kazerun, Iran Departments of Electrical Engineering, Kazerun Branch, Islamic Azad University, Kazerun, Iran Departments of Electrical Engineering, Kazerun Branch, Islamic Azad University, Kazerun, Iran Department of Medical Engineering, Kazerun Branch, Islamic Azad University, Kazerun, Iran 1 12 2021 26 6 941 951 30 08 2021 21 12 2021 Background & aim: MS is a disease of the central nervous system in which the body makes a defensive attack on its tissues. The disease can affect the brain and spinal cord and cause a wide range of potential symptoms, including balance, motor and vision problems. MRI and FMRI images are a very important tool in the diagnosis and treatment of MS. The aim of the present study was to determine and diagnose early diagnosis of MS in MRI images of the brain using deep learning techniques.   Methods: The present experimental study was conducted at Kazerun Azad University in 2020. 1000 images were from BRATS data sets and in the two groups of learning and testing, 70 to 30% were included in the study and a deep four-layer deep learning network based on the network. Convulsive neuralgia is simulated in MATLAB environment. In the deep learning structure, which itself had the ability to extract features, we used another method to do so. For the reason that deep learning, although capable of extracting features was also conducted by chance. In order for the previous steps to be definite, the researchers used another algorithm inside the iteration loops and inside the torsion layer to reduce the dimensions of the feature during the training, secondly to select the best features and thirdly to extract the features definitively.1000 MRI images of BRATS data set in the two groups of learning and testing in the ratio of 70 to 30% were included in the study. A four-layer deep learning network based on convolutional neural network is simulated in MATLAB environment. In the deep learning structure, which had ability itself to extract features, another method was used to do this. For the reason that deep learning has the ability to extract features, but did so randomly. In order for the previous steps to be definitive, another algorithm was used inside the iterative loops and inside the torsion layer to reduce the dimensions of the feature during the training, secondly to select the best features and thirdly to extract the features definitively.   Results: The graphical representation of the ROC curve indicated that the degree of sensitivity or correct prediction against incorrect prediction in this binary classification system in which the separation threshold varies was significant. The area below this curve was 0.8592 and the accuracy of the proposed method was 98.6891 and the sensitivity was 94.8766.   Conclusion: Due to the prevalence of MS disease, early diagnosis and presentation of an intelligent method based on fMRI imaging is essential for treatment. This intelligent method tries to be able to help diagnose and treat more accurately, better identify the features and patterns affecting the disease than previous methods as a physician assistant. Finally, the results obtained from the present study revealed that the efficiency of the proposed method was evaluated at an excellent level and showed its optimality as much as possible. In addition, the obtained results indicated the speed of training and testing of data in high volume and fast convergence of the algorithm. It is correspondingly easier to expand and generalize.   3139 Immunology Professional Rational Drug and Biological Product Design Methods Based on Chemoinformatics as a Novel Science Ranjbar MM Banitaba SMH Mollazadeh SH Motedayen MH Karimi GH Helali Nasab M Department of Immunology, Razi Vaccine and Cold Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran Department of Chemistry, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran, Quality Assurance Group, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran Department of Immunology, Razi Vaccine and Cold Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran Department of Immunology, Razi Vaccine and Cold Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran Department of Microbiology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran, Karaj, Iran 1 12 2021 26 6 952 968 09 08 2021 04 12 2021 Background & aim: The use of Chemoinformatics for drug discovery and development plays an important role in introducing new and more effective drugs, optimizing the properties of existing drugs and the growth of drug-related industries, as well as the health of human, animal and plant communities. Therefore, the purpose of the present study was to determine and evaluate the advanced methods of rational design of drugs and biological products based on modern knowledge of Chemoinformatics.          Methods: The present systematic and meta-analytical review study was conducted in 2020. Chemoinformatics includes logical design, creation, organization, management, retrieval, analysis, dissemination of information, visualization and use of chemical information. This science is not only successful in the discovery and development of chemical drugs, but correspondingly in the development of biological products (vaccines, antibodies). The four main well-developed methods in drug informatics design include; High-yield virtual screening is based on molecular docking, quantitative correlation of activity structure, pharmacophore search, and fragment-based design. Therefore, the methods were reviewed and the advantages and disadvantages were introduced based on the evaluation of the results of studies and related articles.   Results: The results and high quality of analysis of small molecules and biomolecules and evaluations of their interactions and behavior can lead to the cost of time and manpower in achieving the ideal drugs for various diseases. Therefore, it can be of great help not only in the field of treatment and prevention but also in the diagnosis and tracking of infectious and non-infectious agents.   Conclusion: In the present study, effort has been made to clarify new parts of the drug design and engineering with the help of bioinformatics science for its use for Iranian researchers in the field of research and production. This can lead to innovative solutions in accessing better-performing drugs.     3169 Dentistry Prevalence of Dry Mouth (Xerostomia) and Hyposalivation in Individuals with no Oro-Dental Complications in Shiraz, Iran Zahed M Azad A Qapanchi J Ranjbar Z Mansourabadi P Farpour N Mansouri Z Oral Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Oral Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Oral Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Oral Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Oral Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Oral Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Oral Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. 1 12 2021 26 6 969 981 19 09 2021 15 11 2021 Background & aim: Saliva is one of the most important body fluids. The lack of this fluid has many outcomes for the patient. The aim of the present study was to detect objective and subjective dry mouth in individuals with no oro-dental complications in Shiraz, Iran and compare these factors to depression level, medication intake and level of education.   Methods: In the present descriptive cross-sectional study conducted at Shiraz Dental School in 2018, 450 samples were selected from the Patients' companions referred to Shiraz Dental School over 18 years. In the present study, the Fox questionnaire was used to mentally assess dry mouth (zerostomia) and the Zong self-assessment scale was used to assess depression. Spitting method was used to evaluate the amount of stimulated and unstimulated saliva (objective dry mouth or hyposalivation). When saliva was less than 0.1 g/min, objective dry mouth was stimulated and when saliva was less than 0.7g/min, objective dry mouth was considered non-irritating. Data were analyzed using SPSS software and chi-square test.   Results: In the present study, 399 patients (118 males, 280 females) were studied. Dry mouth (zerostomia) was found in 46% of the population. However, 18.5% had non-stimulated objective dry mouth and 12.3% had objectively stimulated dry mouth. Patients aged 30 to 45 years suffered more from mental and objective dry mouth compared to other age groups (p=0.048 and p=0.032). Depression, use of antihypertensive and antidiabetic drugs and low level of education were significantly associated with objective dry mouth (p<0.01, p<0.01 and p=0.038).   Conclusion: Mental dry mouth can occur apart from objective dry mouth. Correspondingly, dry mouth as an objective and mental symptom can be seen not only in the elderly but similarly in the young peoples. Physicians and dentists must deal with the factors that cause this complication, such as; depression, medications, and education levels as a disabling and destructive condition that affects all age groups.     3106 Parasitology Frequency of Trichostrongylus Species and Other Trichostrongylids in Ruminants Slaughtered in Hamadan Slaughter House in 2020 Kashi nahanji M Bakhtiari M Fallah M Department of Parasitology and Mycology, School of Medicine, Hamadan Department of Parasitology and Mycology, School of Medicine, Hamadan Department of Parasitology and Mycology, School of Medicine, Hamadan 1 12 2021 26 6 982 992 20 06 2021 08 11 2021 Background & aim: Zoonotic intestinal parasites and intestinal nematodes of livestock are indirectly one of the human health challenges in developing countries. Considering the possible role of gastrointestinal parasites in domestic animals in the transmission of infection to humans, their economic significance in the production of protein products, and the lack of up-to-date information on the contamination status of ruminants in the region. As a result, the present study aimed at determining the frequency of Trichostrongylus species in ruminants slaughtered at Hamadan slaughterhouse in 2020.   Methods: Abomasum‎ and intestinal samples were taken from 338 livestock by referring to Hamadan slaughterhouse. The samples were placed separately in sealed containers and transferred to the parasitology research laboratory of Hamadan University of Medical Sciences to diagnose parasitic infection. After opening and washing the contents of the samples in a large tray, these contents were filtered through a wire sieve several times and the obtained transparent material was gradually poured into other trays. At that point, the worms were separated with the help of a hand-held magnifying glass and the light of the study lamp and were placed within bottles containing alcohol-glycerin. The worms were then identified at the genus and species levels using diagnostic keys. Information about livestock such as gender, type of livestock, anatomical position, and habitat were recorded in the information form. Data were analyzed using descriptive statistics, and the results were presented as tables and graphs.   Results: Out of 338 livestock, 70 (20.70%), 264 (78.10%), and 4 (1.18%) were Cattles, sheep, and goats, respectively. In addition, 240 (71%) and 98 (28.99%) livestock were male and female, respectively. 301 (89.05%) and 37 (10.94%) livestock were young and old, respectively. The highest percentage of infected specimens was related to Ostertagia Ostertagi (64 cases, 48.5%) and the lowest percentage of infected specimens was related to male Nematodirus filicollis (1 case. 1.6%). Most of the infestations were reported for the male livestock and from those raised in Qorveh area. In addition, no worm infestation was observed incattle and goats.   Conclusion: Ostertagia infection was highly prevalent in the studied livestock. The zoonosis Trichostrongylus parasite was not observed in the present study. The prevalence of intestinal nematodes in sheep in the region is still significant and should be controlled by the relevant authorities.     3100 Clinical Effects of Biofeedback Therapy on Cardiovascular and Respiratory Indices in NREM Sleep Parasomnias with Obstructive Sleep Apnea: A Case Study Khorramdel K Pasalari S Nami M Department of Psychology and Education Science, Fatemiyeh Shiraz, Institute of Higher Education, Shiraz, Iran Department of Psychology and Education Science, Fatemiyeh Shiraz, Institute of Higher Education, Shiraz, Iran Department of Neuroscience, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran 1 12 2021 26 6 993 1007 08 06 2021 26 12 2021 Background & aim: Non-REM parasomnias are a relatively common condition in the general population. Current treatment plans are usually based on small case series and reports. Considering the effects of sleep disorders on different aspects of human life and the failure of pharmacological therapies, the present study was designed to investigate the effect of biofeedback therapy on cardiovascular and respiratory parameters in a case of Non-REM parasomnias with obstructive sleep apnea.   Methods: The present case study was conducted in 2020 on a 60-years-old man with a family history of sleepwalking disorders. The treatment plan in the present study was as follows: execution of the principles of sleep hygiene by the patient, use of continuous positive airway pressure machine (CPAP), and eight weekly biofeedback therapy sessions (including the use of mental exercises for the patient with the use of heart rate sensors, respiration rate per minute, and coordination of respiratory and cardiovascular events). Before the start and after the treatment period, cardiovascular parameters such as blood pressure, arterial blood oxygen saturation, heart rate, and respiratory parameters (apnea, hypopnea, etc.) were measured. Statistical analyses were performed using the delta calculation of changes in indicators and scores obtained from the patient at different stages of the study.   Results: Prior to the interventions, the number of obstructive sleep apneas was 34 times, the number of obstructive hypopneas was 106 times, and the incidence of oxygen desaturation events was 389 times, which accounted for 24% of the total sleep time. After the therapeutic interventions, the obstructive sleep apnea completely disappeared, the number of obstructive hypopneas was reported only 12 times, and the rate of oxygen desaturation events was reduced to 102 times.   Conclusion: The treatment plan in the present study resulted in complete improvement in some parameters such as obstructive sleep apnea and relative improvement in others such as arterial blood oxygen pressure and heart rate. The present study was able to explore and clarify new aspects, both in terms of identifying the mechanisms of the disorder and in terms of treatment.     3107 Psychology Correlation Between Pregnancy Poisoning, Cognitive Development and Symptoms of Behavioral Disorders in Children Bagheri M Hosseini SE Javidi H Sohrabi N Department of Psychology, Islamic Azad University, Marvdasht Branch, Marvdasht, Iran Department of Biology Education, Zand Institute of Higher Education Shiraz, Shiraz, Iran Department of Psychology, Islamic Azad University, Marvdasht Branch, Marvdasht, Iran Department of Psychology, Islamic Azad University, Marvdasht Branch, Marvdasht, Iran 1 12 2021 26 6 1008 1018 23 06 2021 20 11 2021 Background & aim: Maternity events such as preterm birth, low birth weight and low Apgar score can affect postpartum life. Therefore, the aim of the present study was to determine the relationship between pregnancy poisoning and cognitive development and symptoms of behavioral disorders in children.   Methods: The present descriptive correlation study was conducted in 2017. The statistical population included 1500 children born in 2007, 2006 and 2010 at Hafez hospital, Shiraz. The selected sample was 306 according to Morgan table, but due to the samples decrease, 300 children were included in the study. Our sample is limited to children with information about maternal preeclampsia. Cognitive development of these children was measured by a children's Raven color questionnaire with 41% validity and 62% reliability. Symptoms of behavioral disorders were also assessed by the Rutter Behavioral Disorders Questionnaire, a special form for parents with 97% validity and 92% reliability. The collected data were analyzed using Pearson correlation coefficient test.   Results: A significant and positive relationship was observed between pregnancy poisoning and children's cognitive development. The correlation coefficient between the two is 0.187, which is significant at the level of p <0.01. Moreover, the correlation between pregnancy poisoning with aggression is equal to 0.317, with child anxiety is equal to 0.247, with maladaptation is equal to 0.240 and with attention deficit-hyperactivity disorder is -0.212, all of which are significant at the level of p <0.01. It was indicated that there was an inverse and significant relationship between pregnancy poisoning with aggression, anxiety, maladaptation and attention deficit hyperactivity disorder, but the correlation between pregnancy poisoning and child antisocial behavior was 0.107 and no significant relationship was observed.   Conclusion: The results indicated that pregnancy poisoning affects cognitive development and symptoms of behavioral disorders in children. On the other hand, more hospitalization of these infants in the intensive care unit can lead to behavioral disorders in the child in the future.     3079 Gynaecology A Case Report of a Pregnant Patient with Acute Necrotizing Pancreatitis in Imam Sajjad Hospital of Yasuj Asadi Z Mohammadi Salehi Dehno SI Salehi Dehno SI Eslam nik P Department of Obstetrics and Gynecology, School of Medicine, Yasuj University of Medical Science, Yasuj, Iran, Department of Obstetrics and Gynecology, School of Medicine, Yasuj University of Medical Science, Yasuj, Iran, Department of Surgery, Yasuj University of Medical Sciences, Yasuj, Iran. Department of Obstetrics and Gynecology, School of Medicine, Yasuj University of Medical Science, Yasuj, Iran, 1 12 2021 26 6 1019 1026 09 05 2021 18 12 2021 Abstract: Background & aim: Pancreatitis is an inflammation of the pancreas that occurs acutely or chronically. Symptoms of acute pancreatitis include: epigastric pain, nausea, vomiting and in severe cases shock and even death. Pancreatitis should as well be considered as a differential diagnosis in pregnant patients presenting with epigastric pain. Therefore, the aim of the present study was to determine and report a case of a pregnant patient with acute necrotizing pancreatitis in Imam Sajjad Hospital of Yasuj.   Case Report: The patient was a 24-year-old woman, having her first pregnancy with a gestational age of 28-29 weeks. The patient had a history of 3 years of primary infertility following polycystic and hypothyroid ovaries and had been referred to the hospital with symptoms of abdominal pain, nausea and vomiting. The patient was asked for amylase and lipase tests as well as imaging to confirm necrotic inflammation of the pancreas. According to the surgical advice, the patient underwent hydration treatment, lack of oral nutrition, broad-spectrum antibiotics, and finally surgery to remove necrotic tissue. The patient was discharged in good general condition.   Conclusion: Acute pancreatitis during pregnancy can lead to many maternal and fetal complications, including maternal-fetal mortality and preterm delivery, which can be prevented with timely diagnosis and appropriate treatment.