Yasuj University Of Medical Sciences
Armaghane Danesh
1728-6506
1728-6514
22
2
2017
6
1
Functional Comparison of monocytes isolated from culture flask by lidocaine/EDTA, trypsin and cold-PBS/EDTA
145
160
FA
H
Esmaili Gourvarchin Galeh
Department of Microbiology, Urmia University, Urmia, Iran,
h.smaili69@yahoo.com
N
SM
Abtahi Froushani
Department of Microbiology, Urmia University, Urmia, Iran,
meysamabtahi@hotmail.com
Y
N
Afzal Ahangaran
Department of Microbiology, Urmia University, Urmia, Iran,
n.a.ahangran@gmail.com
N
S
Naji Hadai
Solid Tumor Research Center, Urmia University of Medical Sciences, Urmia, Iran.
najihadadi.s@umsu.ac.ir
N
Abstract
Background & aim: Easy access, rapid recovery and high potency of monocyte cell therapy have led to special attention in cell therapy research. Monocytes are considered as sticky cells to the flask. Therefore, finding the appropriate isolation method that has the least damage to the cell and its function is of particular importance. The purpose of this study was to compare the functional capabilities of monocytes after isolation with three methods: lidocaine / EDTA, trypsin and cold PBS / EDTA.
Methods: In this experimental study, after extraction of peripheral blood mononuclear cells from Balb / c mice, cells (107 × 1 cells / ml) were incubated in RPMI culture medium in T25 culture flask for 4 hours. After incubation time, non-adherent cells (mainly lymphocytes) were separated by two rinsing and removed from the flask. Three different methods of trypsin, lidocaine and phosphate buffer saline were used for isolation of monocyte cells. After isolating the cells with each method, the functional capabilities of the monocytes were measured and compared with each other. The collected data were analyzed using one-way ANOVA.
Results: The amount of extraction, survival, metabolic activity, phagocytosis percentage, respiratory explosion, nitric oxide levels, and yeast potential in cells isolated by lidocaine were significantly higher than other groups. Although the use of trypsin, although it results in the removal of more cells, is cooled to the PBS method, but these cells exhibit significant physiological impairment in comparison with the Lidocaine / EDTA or PBS / EDTA method. Neutral red uptake was detected by trypsin isolated monocyte cells in comparison to the other two methods at lower levels. In comparison between cold PBS and lidocaine, it seems that there is no significant difference between the monocytes obtained from the two methods in terms of neutralization.
Conclusion: Compared to trypsin and PBS / EDTA, the Lidocaine / EDTA method is an appropriate method for isolating monocytes adherent to the flask, due to the extraction of more efficient and efficient cells.
.
Isolation, Function, Monocyte, Lidocaine, Trypsin, Phosphate Buffered Saline
http://armaghanj.yums.ac.ir/article-1-1690-en.html
http://armaghanj.yums.ac.ir/article-1-1690-en.pdf
Yasuj University Of Medical Sciences
Armaghane Danesh
1728-6506
1728-6514
22
2
2017
6
1
The Effect of Wheat Sprout Extract on Skin Injury Following Injection of Lead Acetate in Rat
161
175
FA
HR
Moradi
Department of Basic Sciences, University of Tehran, Iran
hmoradi20@yahoo.com
N
H
Morovvati
Department of Basic Sciences, University of Tehran, Iran
hmorovvati@ut.ac.ir
Y
M
Adibmoradi
Department of Basic Sciences, University of Tehran, Iran
adibmoradi@ut.ac.ir
N
H
Najafzadeh Varzi
Department of Basic Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
najafzadeh@scu.ac.ir
N
Abstract
Background & aim: Skin is constantly exposed to environmental contaminants such as heavy metals (lead).Medicinal plants have been concern for the treatment of of human pain. and pain. Wheat Sprout is one of medicinal plants which are rich in vitamins, minerals and powerful antioxidant compounds respectively. The aim of this study was to evaluate the effect of wheat Sprout extract on tissue texture following injection of lead acetate in rats.
Methods: Thirty healthy adult Wistar rats were divided randomly into six groups: Control group received 1 ml/kg/day of normal saline, group 2 received 20 mg/kg/day of lead acetate intraperitoneally respectively, group 3 and group 4 received 100 mg/kg/day and 200 mg/kg/day of wheat sprout extract by gavage feeding, group 5 and group 6 received 100 mg/kg/day and 200 mg/kg/day of wheat sprout extract by gavage feeding along with 20 mg/kg/day of lead acetate intraperitoneally. After five weeks, skin tissue of dorsal region and blood samples were collected for histomorphometric studies and serum assessment. Serum samples were tested for determining antioxidant activity (AOA) based on power ferric reduction antioxidant (FRAP) assay and peroxidation of lipids by measuring malondialdehyde (MDA). The 5 to 6 μm thickness sections were made using paraffin embedding method after stained by hematoxylin and eosin, safranin and masson trichrome. For microscopic study, Dino-Lite digital lens and Dino Capture 2 Software were used.
Results: The lead significantly decreased the total thickness of the skin, the dermal layer, hypoderm, the number and maximum depth of hair follicles and the epidermis pod thickness of the hair root compared with the control group (p <0.05). A significant increase was seen in numbers of sebaceous glands and hair follicles in group receiving 200 mg/kg/day of wheat sprout, compared to that in control group (P <0.001). Wheat sprout simultaneously with lead increased the epidermis sheath thickness of the root (p <0.01) but no significant increase was seen in the depth of hair follicles in comparison with the lead group.MDA level showed a significant increase in lead group, compared to control group (P <0.01). AOA level showed a significant increase in wheat sprout (200 mg/kg/day) group, compared to other groups (P <0.001).
Conclusion: The results showed that lead can induce negative effects in skin tissues. Wheat sprout extract (200 mg/kg/day) can inhibit toxic effects of lead in skin tissues and that leads to refreshing in skin.
Skin, Wheat Sprout, Lead, Histology, Rat
http://armaghanj.yums.ac.ir/article-1-1460-en.html
http://armaghanj.yums.ac.ir/article-1-1460-en.pdf
Yasuj University Of Medical Sciences
Armaghane Danesh
1728-6506
1728-6514
22
2
2017
6
1
The effect of the Movie- Based Education on Sleep Quality in Patients with Bronchial Asthma
176
188
FA
E
Elahiyan Borojeni
Department of Nursing, Yasuj University of Medical Sciences, Yasuj, Iran
elahian.elahe@gmail.com
N
A
Afrasiabifar
Department of Nursing, Yasuj University of Medical Sciences, Yasuj, Iran
afrasiabifar.ardashir@yums.ac.ir
Y
Sh
Najafi Doulatabad
Department of Nursing, Yasuj University of Medical Sciences, Yasuj, Iran
shahlaiss@yahoo.com
N
A
Mousavizadeh
Department of Epidemiology, Yasuj University of Medical Sciences, Yasuj, Iran
Health.epid@gmail.com
N
Background & aim: In recent decades, the use of media education to control the problems of patients with chronic diseases has been taken into consideration by medical staff, but few studies have been done on their effectiveness in improving the quality of sleep in patients with bronchial asthma. The aim of this study was to determine the effect of film-based education on sleep quality in patients with bronchial asthma.
Methods: The present study was a quasi-experimental study and all of the patients with bronchial asthma referred to the Asthma clinic of Boroujen were included in this study. Fifty one patients who were eligible for inclusion in the study were selected by non probability sampling method. The samples were assigned to randomly assigned blocks between the two test and control groups. For patients in the test group, patient education was performed through a 15-minute video clip by computer, DVD player and mobile phone, but patients in the control group only received routine care. The Pittsburgh Sleep Quality Index questionnaire was used to collect data one week before intervention and one month after intervention. The collected data were analyzed by descriptive statistics and t-test, Chi-square, Mann-Whitney and Wilcoxon tests.
Results: There was no significant difference before the intervention, in the overall score of sleep quality and its dimensions in the two groups (p = 0.66). There was a significant difference between the two groups after the intervention in the general score of sleep quality and the dimensions of sleep quality, sleep disturbances and sleep latency (p = 0.001) However, there was no significant difference in the use of sleep apnea, sleep duration, adequacy of sleep and daily dysfunction in the test and control groups.
Conclusion: The movie-based instruction has been able to improve the quality of sleep in patients with bronchial asthma. Considering the ease of implementation of this educational method, suggesting other researches to evaluate its effect on other patients with chronic diseases, it should be considered as a method of patient education for self-care at home.
Bronchial Asthma, Movie- based Education, Patient Education, Sleep
http://armaghanj.yums.ac.ir/article-1-1761-en.html
http://armaghanj.yums.ac.ir/article-1-1761-en.pdf
Yasuj University Of Medical Sciences
Armaghane Danesh
1728-6506
1728-6514
22
2
2017
6
1
Therapeutic effect of Aerobic Training on Cross-Linked Telopeptides of Type I Collagen (CTX) as Bone Reasorption Indicative in Obese Male with Mild to Moderate Asthma
189
204
FA
H
Ghasemalipour
Department of Physical Education and Sport Science, South Tehran Branch, Islamic Azad University, Tehran, Iran,
a.alipour66@yahoo.com
N
M
Eizadi
Department of Exercise Physiology, Saveh Branch, Islamic Azad University, Saveh, Iran,
izadimojtaba2006@yahoo.com
Y
MH
Dadgan
Department of Physical Education and Sport Science, South Tehran Branch, Islamic Azad University, Tehran, Iran,
m_dadgan@yahoo.com
N
N
Shakeri
Department of Exercise Physiology, Science and Research Branch, Islamic Azad University, Tehran, Iran
n.shakeri@srbiau.ac.ir
N
Background & aim: Long-term usage of inhaled corticosteroids is associated with osteoporosis in asthma patients. The purpose of this study was to determine the effect of long-term aerobic exercise on C-type collagen type T-terminal (CTX) as an indicator of bone resorption and serum calcium levels in asthmatic patients treated with inhaled corticosteroids.
Methods: For this purpose, 24 obese asthma patients treated with inhaled corticosteroids with a mean age of 38 ± 7 years and body mass index of 31.5 ± 3.15 kg / m2 were randomly divided into experimental(Without exercise) and aerobic training groups. The experimental group participated in an aerobic exercise program for 3 months in 3 sessions ranging from 45 to 60 minutes in the range of 55-75% of maximum heart rate weekly. Fasting samples were taken in both groups before and after intervention for measuring CTX and calcium levels. In order to compare the data in pre-test between two bgroups, independent t-test was used for two groups. t-test was used for determining the intra-group variation in the t test for significance level of t test p <0.05.
Results: Aerobic intervention led to a significant reduction in weight, body mass index and body fat percentage in the experimental group (p <0.05) but CTX levels decreased after aerobic training compared to baseline levels (p = 0.002). Aerobic exercises also led to a significant increase in serum calcium (p <0.001), but this variable did not change in the control group.
Conclusion: A long-term aerobic intervention can induce favorable effect on bone turn over by improved bone resorption indicatives in asthma patients. Based on this finding, regular aerobic training is purposed to prevent and decrease osteoporosis intensity in corticosteroid-treated asthmatic patients.
Aerobic training, Asthma, Osteoporosis, Cross-linked telopeptides of type I collagen
http://armaghanj.yums.ac.ir/article-1-1685-en.html
http://armaghanj.yums.ac.ir/article-1-1685-en.pdf
Yasuj University Of Medical Sciences
Armaghane Danesh
1728-6506
1728-6514
22
2
2017
6
1
Effects of rhythmic aerobic exercise plus core stability training on serum chemerin levels and Glycemic index of overweight women
205
219
FA
Z
Taghavian
Department of Exercise Physiology, Shahrekord University, Shahrekord, Iran
zamani.h1347@gmail.com
N
H
Rabiee
Department of Exercise Physiology, Shahrekord University, Shahrekord, Iran
hajar.rabie@yahoo.com
N
M
Faramarzi
Department of Exercise Physiology, Shahrekord University, Shahrekord, Iran
dr.md1144@gmail.com
Y
A
Azamian
Department of Exercise Physiology, Shahrekord University, Shahrekord, Iran
sabanouri888@yahoo.com
N
Background & aim: Chemerin are novel adipokines that are secreted from adipose tissue and improved insulin sensitive. The purpose of this study was to examine the ffects of rhythmic aerobic exercise plus core stability training on serum chemerin levels and Insulin resistance, glucose levels and body composition of overweight women.
Methods: In this quasi-experimental study, 40 healthy women aged 25-45, waist circumference equal to and above 88 cm, body mass index of 3025 kg / m2 were selected and randomly divided into control (n = 16) and experimental (n = 19) groups. ). Five people were excluded from the study. The experimental group was trained 12 weeks (3 sessions per week per session). The exercise program included aerobic exercise (55% -80%), maximum heart rate, and trunk stability training. The pre-test and post-test stages were collected at 12-hour fasting time, blood samples were collected in the same conditions. The ELISA method was used to determine the fasting and low-level insulin levels. Insulin resistance was evaluated based on the evaluation of HOMA-IR model of homeostasis. To compare the pre-test and post-test changes, the dependent t-test was used and the inter-group variations of one-way ANOVA with a significant level (p≤0.05) were used.
Results: Chemerin plasma levels, body mass index, weight, body fat percentage and The ratio of waist circumference to hip In Overweight After 12 weeks Rhythmic aerobic exercise Along with Trunk stability exercises Showed a significant reduction )p≤0/05). But insulin resistance The fasting plasma glucose and Insulin levels between two groups Practice and Control was no significant difference (p>0/05).
Conclusion: Despite the fact that this kind of intervention can be an effective treatment to improve the level of low-fat adipokine and lower body waist circumference, however, a trickier exercise is required to significantly change insulin resistance and glucose levels In women with overweight.
Aerobic exercise, Trunk stability, Chemerin, Insulin resistance, Obesity
http://armaghanj.yums.ac.ir/article-1-1629-en.html
http://armaghanj.yums.ac.ir/article-1-1629-en.pdf
Yasuj University Of Medical Sciences
Armaghane Danesh
1728-6506
1728-6514
22
2
2017
6
1
with gingivitis and periodontitis referring Resalat Dental Clinic, Chaleshtor in 2015
220
229
FA
MS
Khafari ghosheh
Student Research Committee, Shahrekord University of Medical Sciences, Shahrekord, Iran
heidari_62@yahoo.com
N
B
khalili
2Department of Parasitology and Mycology, Shahrekord University of Medical Sciences, Shahrekord, Iran,
bahman_khalili@yahoo.com
Y
S
Kheri
Department of Biostatistics, Shahrekord University of Medical Sciences, Shahrekord, Iran,
journalism.group@yahoo.com
N
A
Shataghi
Department of Dentistry, University of Medical Sciences Shahrekord Medical Sciences, Shahrekord, Iran
MAJID.JAHANBAZI@YAHOO.COM
N
S
Heidari sorshjani
Social Determinants of Health Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
N
Abstract
Background & aim: Entamoeba gingivalis and Trichomonas tenax are oral protozoa that could cause periodontitis and gingivitis. The present study was done to determine the prevalence of these two protozoa in people over 14 years with periodontitis and gingivitis.
Methods: In this descriptive, cross-sectional study, 540 patients referring Resalat Dental Clinic, Shahrekord were enrolled and assigned in two groups of 270 patients with periodontitis and gingivitis and270 healthy individuals. The prepared specimens were examined by extensive wet procedures, Gimsa staining and Trichorom staining. Data were analyzed by chi-square, Fisher’s exact test, and logistic regression in SPSSv.20.
Results: No E. gingivalis- and T. tenax-positive cases were seen in the healthy group. The prevalence of E. gingivalis and T. tenax was obtained 3% by extensive wet procedure, 1.9% by Trichoderma staining, and 0.7% by Giemsa staining respectively. By logistic regression model, none of variables of age, gender, place of residence, smoking, tooth brushing, flossing, and oral PH were associated protozoan infection of E. gingivalis and T. tenax (P>0.05).
Conclusion: In patients with periodontitis and gingivitis referred to the dental clinic, parasitic infections were attenuated to gingivialis and trichomoniasis vaginalis, and possibly other non-parasitic agents, including bacteria or other microorganisms, may play a role.
Entamoebagingivalis, Trichomonastenax, Gingivitis, Periodontitis
http://armaghanj.yums.ac.ir/article-1-1708-en.html
http://armaghanj.yums.ac.ir/article-1-1708-en.pdf
Yasuj University Of Medical Sciences
Armaghane Danesh
1728-6506
1728-6514
22
2
2017
6
1
Effectiveness Cognitive – Behavioral on Academic Enthusiasm of Students Under the Supervision of the State Welfare Organization
230
241
FA
Abbasi
1Department of Psychology, Salman Farsi University of Kazerun, Kazerun, Iran
shahriardargahi@yagoo.com
N
A
Iraji
Department of Psychology, Yasooj Branch, Islamic Azad University, Yasooj, Iran,
: Amaneh.iraji@gmail.co
Y
K
Khazan
Department of Counseling, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran,
N
D
Azimi
Department of counseling, University of Allame Tabatabaii, Tehran, Iran
N
Abstract
Background & aim: Academic achievement and students' academic achievement are one of the key issues affecting academic eagerness. The purpose of this study was to evaluate the effectiveness of cognitive-behavioral therapy on the students' academic well-being. In this research, 40 randomly selected sampling students were selected as case study in two experimental and control groups of 20. For data collection, Frederick et al (2005) educational agility questionnaire was used and the cognitive-behavioral therapy protocol was applied to the students of the experimental group. MANOVA Multivariate Analysis of variance (ANOVA) was used to analyze the data.
Methods: The research was semi-experimental with pretest, posttest and control group. The study sample included all female students under the supervision of well-being in Yasuj. In this study, using simple random sampling method, 40 students under the supervision of well-being in both experimental and control groups (for each subgroup of 20 patients) were selected as sample. To collect information from questionnaires academic enthusiasm Frederick et al (2005) and the Protocol on the students in the experimental group were administered cognitive-behavioral therapy. Multivariate analysis of variance for data analysis (MANOVA) was used.
Results: Based on the results of MANOVA, cognitive-behavioral therapy in the post-test the emotional longing, the longing cognitive, and behavioral enthusiasm and passion for the school, had a significant effect. Size 0.67 is the effect of CBT on academic enthusiasm. According to post-test scores of experimental and control groups, Cognitive-behavioral therapy enhances students' educational aspirations under the supervision of well-being in the experimental group compared with the control group in the posttest.
Conclusion: The direct and indirect use of cognitive-behavioral training can help students to ease and encourage to school and study.
Cognitive-behavioral therapy, Academic enthusiasm, Students
http://armaghanj.yums.ac.ir/article-1-1317-en.html
http://armaghanj.yums.ac.ir/article-1-1317-en.pdf
Yasuj University Of Medical Sciences
Armaghane Danesh
1728-6506
1728-6514
22
2
2017
6
1
Modeling the Structure of the Relationship Between Emotion Regulation Difficulties, Positive and Negative affect Sleep Quality
242
254
FA
S
Amiri
Department of Psychology, Urmia University, Urmia, Iran
amirysohrab@yahoo.com
Y
A
Isazadegan
Department of Psychology, Urmia University, Urmia, Iran
N
Abstract
Background & aim: Poor quality sleep is very common in modern societies and has a significant negative impact on psychological and physiological Dimensions. The purpose of the present study was to investigate correlational relationships and draw up a positive and negative affect mediation model between the difficulty in regulation of emotion and quality of sleep.
Methods: In this descriptive-correlational study, the participants of the study were selected through multi-stage cluster sampling. Then, the difficulty questionnaire was distributed in emotion regulation, affective and negative and sleep quality among the participants . Collected data was analyzed for descriptive, correlation, structural equation modeling to investigate the research objectives.
Results: The results showed that there was a significant positive correlation between the difficulty components of emotion regulation and negative emotion with poor sleep quality and positive correlation between positive affection and high sleep quality (p <0.01). Also, the pattern of structural equation modeling indicates the role of mediating positive and negative affects in the relationship between the difficulty in regulation of emotion and sleep quality .
Conclusions: The results supported the hypothesis that the difficulty in regulation of emotion interferes with the quality of sleep, and positive affection can have a moderating role in this regard.
Emotion regulation difficulties, Positive affect, Negative affect, Sleep quality, Structural equation modeling
http://armaghanj.yums.ac.ir/article-1-1512-en.html
http://armaghanj.yums.ac.ir/article-1-1512-en.pdf
Yasuj University Of Medical Sciences
Armaghane Danesh
1728-6506
1728-6514
22
2
2017
6
1
Spiritual Perception Of Women From Labor Pain
255
270
FA
P
Yadollahi
Department of Midwifery,Islamic Azad University, Kazeroon Branch, Kazeroon ,Iran
yadollahi_556@yahoo.com
Y
Z
Taghizdeh
2Department of Midwifery, School of nursing and midwifery, Tehran University of Medical Science, Tehran, Iran
ebadi1347@yahoo.com
N
A
Ebadi
3Behavioral Sciences Research Center, Nursing Faculty, Baqiyatallah University of Medical Sciences, Tehran, Iran
zibataghizadeh@yahoo.com
N
M
Gharacheh
4Department of Midwifery, Islamic Azad University, Gachsaran Branch, Gachsaran, Iran
gharacheh.m@ gmail .com
N
Abstract
Background & aim: Consciously and actively experiencing labor pain can be associated with spiritual development and understanding of individual strengths and provide an opportunity for women to always have pleasant memories of childbirth. The present study aimed to explore women’s spiritual experiences of labor pain.
Materials & Methods: This is a qualitative study conducted on a purposeful sample of 17 women with the experience of normal childbirth. Data were collected by semi-structured, in-depth interviews. The interviews were recorded, transcribed and analyzed using content analysis approach proposed by Graneheim and Lundman.
Results: The participants were 17 women, with a mean age of 33 years and the majority of them had undergraduate education or higher and middle-level income. Sub-categories emerged from this study included “the duality of feelings”, “positive insight”, “self-steam” and “spirituality” that generated the main category of “transcendence by experiencing labor pain”.
Conclusion: According to recent policies of the Ministry of Health in order to make normal vaginal delivery pleasant and considering the Iranian cultural and religious background about the sanctity of natural childbirth, profound and spiritual understanding of the experience of labor pain can help to develop appropriate programs and supportive practices for the laboring women, resulting in maintaining human greatness.
.
Labor pain, Perception of pain, Spiritual
http://armaghanj.yums.ac.ir/article-1-1531-en.html
http://armaghanj.yums.ac.ir/article-1-1531-en.pdf
Yasuj University Of Medical Sciences
Armaghane Danesh
1728-6506
1728-6514
22
2
2017
6
1
The characterization Molecular of cry genes in the Bacillus thuringiensis isolated from soil
271
281
FA
R
Mahmoudi
Department of Toxicology, Shahreza Branch, Islamic Azad University, shahreza, Iran
rezamahmoudi6969@yahoo.com
N
GH
Irajian
Department of Microbiology, Iran University of Medical Sciences, Tehran, Iran,
girajian@yahoo.com
N
I
Javadi
Department of Toxicology, Shahreza Branch, Islamic Azad University, shahreza, Iran
Y
E
Panahi kokhdan
Department of Biology, University of Guilan, Rasht, Iran
esmaeel_panahi@yahoo.com
N
Background & aim: Bacillus thuringiensis (Bt) is a Gram-positive and sporulating bacteria. The main habitat of the bacteria is soil and able to produce a wide variety of insecticidal crystal protein's with Insecticide properties. The aim of the study was to detection of cry1, cry2, cry3, cry4 and cry5 genes in the B. thuringiensis isolated from soil samples by Multiplex-PCR method.
Methods: In this cross-sectional study, 200 grams of soil sample randomly were collected from at 10 cm depth and totally 100 soil samples were taken from different areas of Tehran, Iran. According to the protocol approved by WHO, suspected colonies on B. thuringiensis isolated and strains were confirmed by the microbiological and biochemical tests. After DNA extraction Multiplex-PCR using specific primers in thermal cycler gradient the presence of cry genes were confirmed. Data were analyzed using one-way ANOVA.
Results: Out of 100 soil samples studied, 41 strains were confirmed as B. thuringiensis. MPCR results showed that 14 strains carrying the crystalline toxin, 7 strains (50%) cry1, 4 strains (5.28%) cry2 and 3 strains (5.21%) cry3 genes. Cry4 and cry5 genes were not observed in any isolates (0.0%).
Conclusion: Based on great diversity of crystal protein genes and the absence of reliable phenotypic methods for detection of the presence of crystal proteins, simultaneous identification of these genes can be found as low cost and high speed response for identification of crystalline genes produced by B. thuringiensis.
Bacillus thuringiensis, cry genes, Multiplex-PCR
http://armaghanj.yums.ac.ir/article-1-1412-en.html
http://armaghanj.yums.ac.ir/article-1-1412-en.pdf