per
Yasuj University Of Medical Sciences
Armaghane Danesh
1728-6506
1728-6514
2015-11
20
8
651
665
article
Comparison of therapeutic effects of bone marrow mesenchymal stem cells and liquid culture environment (secreta) in the treatment of induced knee abrasion created in guinea pigs
MR Sadraie
mreza_sadraie@yahoo.com
1
D Mehrabani
MEHRABAD@SUMS.AC.IR
2
A Vahdati
medvetbiochem93@gmail.com
3
Department of Animal Sciences, Fars Research and Sciences Branch, Fars, Iran
Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Department of Animal Sciences, Fars Research and Sciences Branch, Fars, Iran
Background and aim: Osteoarthritis (OA) is a common disease with unknown causes which is related to the age and is more common in middle and older age. The aim of this study was to evaluate the effect of bone marrow derived mesenchymal stem cells (BM-MSCs) and secreta in healing of induced OA in guinea pig. Methods: BM-MSCs were extracted from guinea pig bone and cultured. OA was induced by cutting the anterior cruciate ligament in 15 guinea pigs. Then, 106 BM-MSCs at 3rd passage were administered to 5 animals, secreta was injected to 5 other and 5 were kept as the control group as untreated. After three months, the healing processes were evaluated by testing of histopathology and radiological parameters. Results: The radiological assessment showed a significant reduction of OA in stem cells and secreta groups in comparison to the control group (P<0.05). Also, OA histological feature in stem cells and secreta groups was better than control group. However, the matrix distribution of articular cartilage and collagen types 1 and 2 in secreta group were significantly better than other groups (P<0.05). Conclusion: Our results showed that the use of BM-MSCs and their secreta in treatment of OA was associated with reduction of radiological and histological index of OA.
http://armaghanj.yums.ac.ir/article-1-915-en.pdf
Osteoarthritis
bone marrow derived mesenchymal stem cells
secreta
healing.
per
Yasuj University Of Medical Sciences
Armaghane Danesh
1728-6506
1728-6514
2015-11
20
8
666
676
article
Prediction and cloning linear Tcell epitopes of P14-3-3 antigen into pEGFP–N1 as a DNA vaccine model to induse immuno response against hydatidosis and it\'s expression in CHO cell line
R mesri
1
M Esmaelizad
m.esmaelizad@rvsri.ac.ir
2
SA Angaji
3
M Tahmaseb
4
M Ahmadzadeh
5
S Mohammadi
6
1Department of Cell and Molecular Biology, Kharazmi University, Tehran, Iran
2Department of Central labratoary, Razi Vaccine and Serum Research Institute, Karaj, Alborz, Iran.
1Department of Cell and Molecular Biology, Kharazmi University, Tehran, Iran
1Department of Cell and Molecular Biology, Kharazmi University, Tehran, Iran
1Department of Cell and Molecular Biology, Kharazmi University, Tehran, Iran
1Department of Cell and Molecular Biology, Kharazmi University, Tehran, Iran
ABSTRACT
Background & purpose: Hydatidosis is a zoonotic disease that caused by infection with the larvae of Echinococcus granulosus. Different antigens produced in larval stage of this parasite that recombinant vaccine base these antigens created significant immunity in infected animals. One of the important antigens is p14-3-3 that it's recombinant antigen created considerable immunity in mouse models. In this study according to the high immunity of antigen epitopes region the coding sequence of T-cell epitopes of P14-3-3 was cloned into pEGFP-N1vector in order to produce an effective DNA vaccine model to stimulate high level of Th1 immune response.
Material and method: In this study bioinformatics tools were used to prediction of linear T-Cell epitopes of Echinococcus granulosus P14-3-3 &zeta antigen. The nucleotide coding sequence of these epitopes was synthesized by PCR. the ampliqon was digested with XhoI restriction enzyme and cloned into pEGFP–N1 vector That has been purificated by modified sambrook method with CaCl2 and PEG6000..Positive colony was selected by direct colony PCR and confirmed by the sequencing.and evaluation of it's expression in Eukaryotic cells was done by transformed to CHO cell line with electroporation.
Results: Linear T-cell epitopes of Echinococcus granulosus P14-3-3 after prediction,synthesis and amplification wae successfully cloned into pEGFP-N1 vector that purificated by new method with maximum vector and minimum RNA concentration.The expression of new constract in CHO cell line as a eukaryotic cells achivment by fluorescent microscope and will be used as a DNA vaccine model to evaluation immuno response in mouse models.
Discussion: Successfully cloning of The linear T-cell epitppes coding sequence of Echinococcus granulosus P14-3-3&zeta antigen into pEGFP-N1 verificated by sequencing and fluorscent microscope images demonstrated expression of recombinant protein in CHO cell line
http://armaghanj.yums.ac.ir/article-1-761-en.pdf
Echinococcus granulosus
DNA vaccine
cloning
P14-3-3
per
Yasuj University Of Medical Sciences
Armaghane Danesh
1728-6506
1728-6514
2015-11
20
8
677
688
article
Study of protective effect of Avicennia marina hydroethanolic leaf extract on testes tissue and spermatogenesis in male rat induced with carbon tetrachloride
Z soleimani
zohre.solymani@gmail.com
1
N mirazi
mirazi205@gmail.com
2
Biology Department, Faculty of Basic Sciences, Bu-Ali Sina University, Hamedan, Iran
Biology Department, Faculty of Basic Sciences, Bu-Ali Sina University, Hamedan, Iran
Aim: The toxic chemical compounds are widelyused in the world. Carbon tetrachloride which is used in hygiene industries caused tissue disorders. Medicinal plants have protective effects in body tissues. In this study the protective effect of Avicennia marina leaf extract (MLE) on spermatogenesis in male rat were induced with ccl4 investigating. Method and materials: The 42 male rats with 220-250 gr body weight were divided randomly in 6 groups(n=7): control (taking normal saline,0.5ml/day, i,p ), sham(taking olive oil, 0.5ml/day, i,p single dose),group induced by ccl4(carbon tetrachloride 1:1 with olive oil,0.5ml single dose, i,p), treated groups: (1,2 and3 by carbon tetrachloride 1:1 with olive oil,0.5ml single dose and 200mg/Kg, 400mg/Kg and 800mg/kg MLE /day for 96 hrs, i,p). After the examination the blood samples were collected from heart directly and testosterone , FSH, LH , sperm count, sperm motility and GSI were analyzed and the microscopic studies of testes tissue were done. All data were expressed as mean±SEM. and statistical significance differences were accepted at P<0.05. Results: Our results showed that the carbon tetrachloride has necrotic effect in testes. The number of sperm and motility were increased and microscopic study of testes tissue showed the necrosis and inflammation with decrease in spermatogonia and spermatocytes comparedwith ccl4 induced only group significantly (P<0.001) and treated groups were no changed. Conclusion: the Avicennia marina hydroethanolic extract has antioxidant and flavonoids compounds which can protect the testes tissues from toxic chemical agents.
http://armaghanj.yums.ac.ir/article-1-951-en.pdf
Avicennia marina
spermatogenesis
testosterone
ccl4
rat
per
Yasuj University Of Medical Sciences
Armaghane Danesh
1728-6506
1728-6514
2015-11
20
8
689
705
article
Emergence of Multidrug-Resistant Pseudomonas aeruginosa: Detection of Isolates harboring blaCTX gene causing infections in hospital and determination of their susceptibility to antibiotics
Z Rabani
rabani_malihe@yahoo.com
1
J Mardaneh
Jalalmardaneh@yahoo.com
2
1Department of Microbiology, Fars Science and Research Branch, Islamic Azad University, Shiraz, Iran. 2Department of Microbiology, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
1Professor Alborzi Clinical Microbiology Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, Iran.
Background & aim: Because of its ubiquitous nature, ability to survive in moist environments, and innate resistance to many antibiotics and antiseptics, P. aeruginosa is a common pathogen in hospitals. The goals of this study were detection of Psudomonas aeruginosa harboring blaCTX gene causing infections in hospitals and determination of their susceptibility to antibiotics and ESBL production.
Methods: In the present cross-sectional study, clinical samples from hospitalized patients were collected and culture was done on apropriate media. Final identification was performed using biochemical tests and API 20NE system. According to the protocol CLSI 2014 disc diffusion, combination disk, modified hodge test (MHT) and E-test were used for antibiotic susceptibility, ESBL production, carbapenemas production, and MIC values of imipenem respectively. The blaCTX gene was detected in the isolates by PCR molecular method.
Results: In the current study, 45 isolates of Pseudomonas aeroginosa were obtained from hospitalized patients, consisting of 19 males (42.2%) and 26 females (57.8%). As observed, 57.8% (26 strains) of isolates were recovered from sputum. The most effective antibiotics against isolates were amikacin and colistin with 97.8% suseptibility whereas the highest resistance was to cefotaxime (97.8%). As revealed 77.8% of isolates showed response to group 2 carbapenems (imipenem, meropenem). All imipenem resistant strains had the MIC more than 32. Seventeen strains (37.7%) were showed resistant to quinolones (ciprofloxacin, norfloxacin). The results of PCR on blaCTX gene indicated that 15.5% of the isolates possess the gene.
Conclusion: Carbapenem group of antibiotic in 22% of infections caused by Pseudomonas aeruginosa were ineffective and indiscriminate prescribing of these drugs will increase the ratet of resistance.
http://armaghanj.yums.ac.ir/article-1-923-en.pdf
Hospital infection
Pseudomonas aeruginosa
Multidrug-resistant (MDR)
blaCTX gene
antibiotic susceptibility.
per
Yasuj University Of Medical Sciences
Armaghane Danesh
1728-6506
1728-6514
2015-11
20
8
706
719
article
Evaluation antiviral effect of leaf extract of Rosmarinus officinalis against Herpes simplex virus type 1 in cell cultur
G Yousefi Kordestani
masoud_parsania@yahoo.com
1
M Parsania
golnaz_y9@yahoo.com
2
Islamic Azad University, Tehran Medical Sciences Branch
2Department of Microbiology, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran.
Introduction Herpes simplex virus type I belongs to herpesviridae. Several studies are ongoing for achieve a drug with good effectiveness and lower side effects, because the incidence of infections caused by this virus is increasing and there is some HSV resistance in the world. In this study, anti-viral effects of rosemary extract against of HSV was evaluated in cell culture. Material and methods: At first the rosemary extract toxicity on Hela cells with trypan blue and MTT methods has been examined and the maximum range of non-toxic concentration on cells has been achieved. At next stages of study direct effect of this concentration of extract examined and after that different concentration of extract in different periods of virus replication have been evaluated. Virus titer with TCID50 method has been measured. Results: Results show, 0.6 mg/ml concentration of rosmary extract is non-toxic on Hela cells and has the maximum effect to prevent HSV-1 replication. The maximum effect of extract has been showed is on immediately after virus adsorption and 1hour after cells infection. In this condition virus titer has decreased compared to other times of virus replication. Conclusion: In accordance with results of this study, herbal basic materials perform their anti-virus effect through interfere with alpha and beta genes expression.
http://armaghanj.yums.ac.ir/article-1-682-en.pdf
Rosemary
Herpes Simplex Virus Type 1
HeLa Cell
per
Yasuj University Of Medical Sciences
Armaghane Danesh
1728-6506
1728-6514
2015-11
20
8
720
731
article
The Effect of Insulin-like Growth Factor-1, Cysteamine and β-Mercaptoethanol on the In Vitro Maturation of Immature Mice Oocytes
A Dehghan Manshadi
1
M Rostami Hosseinkhani
2
M Nikseresht
3
A Hedayatpoor
4
M Akbartabar toori
5
S Mehrabi
6
R Mahmoudi
rmahmoudi40@yahoo.com
7
1Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran
1Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran
Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
departmant anatomy University of Medical Sciences, Tehran, Iran
4Social Determinants of Health Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
2Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
Background & aim: In vitro maturation of oocytes is a promising technique for reducing the costs and complications of ovarian stimulation by gonadotropins. The aim of this study was to investigate the effects of combination of insulin-like growth factor-1 and antioxidant cysteamine and &beta-Mercaptoethanol on maturation and fertilization of immature oocytes.
Methods: in this experimental study, following 48 hrs injection of 7.5 IU PMSG to immature female mice, the germinal vesicle oocytes from ovaries were removed and transferred to TCM199 culture medium containing 50 ng /ml insulin-like growth factor-1 and 100 &mumol Cysteamine and &beta -Mercaptoethanol. After 24 hrs of culture, the oocytes of MII in IVF were fertilized and embryonic development to the two cells was studied under an inverted microscope. Data analysis was performed by using ANOVA and Post hoc Tukey test.
Results: The results showed that the rate of maturation, fertilization and 2-cell embryo formation in GV oocytes with cumulus cells in TCM199 medium containing insulin-like growth factor-1, Cysteamine and BME were 92.10, 93.30, 80.60% and in the GV oocytes without Cumulus cells were cultured in the same medium were 65.80, 64.00, 58.60% respectively which were statistically significant (P <0.001).
Conclusion: In the present study, the simultaneous combination of insulin-like growth factor-1, &beta-Mercaptoethanol and CYS increased maturation, fertilization and developmental rate to 2-cells stage with cumulus cells more than the oocyte without cumulus cells to a greater extent. This represented the need of adding supplemental growth factors and antioxidants to the medium and is associated with cumulus cells.
http://armaghanj.yums.ac.ir/article-1-1135-en.pdf
Germinal vesicle oocytes
insulin-like growth factor 1
Cysteamine
Beta-Mercaptoethanol
Cumulus cell
in vitro maturation
per
Yasuj University Of Medical Sciences
Armaghane Danesh
1728-6506
1728-6514
2015-11
20
8
732
743
article
Comparison of the eight weeks of supplementation Creatine and Glutamine consumption along with resistance exercise on the level of ALP in female mice
A eskandari
abasqqa2014@gmail.com
1
Gh Sharifi
gre_sharifi@yahoo.com
2
M Modaresi
mehrdad_modaresi@hotmail.com
3
Faculty of Physical Education and Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
Faculty of Physical Education and Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
Faculty of Physical Education and Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
Background and purpose: in recent years, in order to improve power, speed, the increase in the volume of the musculature, preventing sports injuries and maintain the muscle performance athletes use from different resistance exercises and food supplements. In this regard, present study has been conducted with the aim of comparison the influence of an 8 week period consumption of creatine (2 gr.kg-1.day-1 in 1st week and 0.48 gr.kg-1.day-1during 2nd to 8th weeks) and glutamine (1 gr.kg-1.day-1 from first to eighth weeks) along with resistance exercise on level of ALP of female mice. Materials and methods: This experimental study was done on 80 Small adult female mice of Surrey species (28 ± 5 gram). The animals were randomly divided into 8 groups of: resistance exercise, resistance exercise + creatine, resistance exercise + glutamine, resistance exercise + glutamine + creatine, creatine, glutamine, creatine + glutamine and control groups (N= 10). Resistance exercise (5 days a week) was including: climbing (4 sets, 5 times repetition with two minutes rest between the sets) from a ladder (with the height of one meter and including 26 steps) and bearing 30 percent of the weight of the Mouse body (hanging from tail) in the first week and the increasing it up to 200 percent of body weight till the last week of the experiment. During 48 hours after the last practice session of resistance exercise, the blood sample was taken and the the level of ALP has been measured. Findings:The results showed that the level of ALP enzyme in creatine + glutamine + resistance exercise groug had been increased in comparison with the control group (144.3 ± 15.86 in comparison with 234.7 ± 25.69 U.L-1 P < 0.05). Conclusion: The results of this research indicate Creatine and Glutamine supplementation consumption along with resistance exercise increases in the level of ALP enzyme in the liver of mice.
http://armaghanj.yums.ac.ir/article-1-681-en.pdf
ALP enzyme
liver
creatine
glutamine
resistance exercise
female mice.
per
Yasuj University Of Medical Sciences
Armaghane Danesh
1728-6506
1728-6514
2015-11
20
8
744
755
article
The Study of early maladaptive Schemas in Men Dependent on Drugs and those Not Dependent
MR Firoozi
m.firoozi@yu.ac.ir
1
SH Kharamin
2
S Ahmadi
3
F Panahi
f.panahi@yahoo.com
4
1Department of Psychology, University of Yasuj, Yasuj, Iran
Social Determinants of Health Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
3Department of Sociology, University of Yasuj, Yasuj, Iran
Islamic Azad university, Yasuj branch, Yasuj, Iran
Introduction and Objectives of the Study Dependence on drugs is a prevalent problem throughout the world, in general and in Iran, in particular. Such a phenomenon is associated with numerous negative consequences. Given the changes in the consumption patterns in different countries, especially in Iran, and the abuse of drugs, identifying the factors which may pave the way for drug abuse is absolutely essential, which can be taken into account in setting the objectives of therapy programs. Cognizant of this, the present study has sought to study early maladaptive schemas in men dependent on drugs and those that are not. Materials and Method: The research design adopted in the present study was causal-comparative. The populaiton of interest was all the men dependent on drugs in the city of Yasouj, who had presented to recovery centers affiliated to welfare organization and Yasouj University of Medical Sciences in the year 2014 and were undergoing treatment. Using multi-stage cluster sampling, initially out of 23 centers for recovery programs, four centers were randomly chosen. Following that, out of each center, 20 and in total, 80 clients were chosen as the sample through convenience sampling. In addition, 80 men not dependent on drugs were chosen through matching with the sample dependent on drugs in terms of age, gender and locality. For the purpsoe of measuing early maladaptive schemas, we made use of the short version of Young’s early maladaptive Schemas Questionnaire. In order to determine the prevalent schemas in men dependent on drugs and those not dependent and the difference between the two groups, use was made of independent-sample t-test and effect size (d). Findings: The findings suggest that mean values of those dependent on drugs in all the schemas in quesiton were significantly higher than those of men not dependent. Although the difference in effect size of all schemas fell in the domain of the large effect, the largest difference in effect size between the two
http://armaghanj.yums.ac.ir/article-1-199-en.pdf
dependent