Armaghane Danesh

fa تأثیر آنتی‌باکتریال عصاره هیدروالکلی گیاه هلپه(Teucrium polium L.) بر سویه‌های استاندارد و بالینی اشریشیاکلی در مقایسه با آنتی‌بیوتیک‌های متداول In Vitro Antibacterial Activity of Teucrium Polium L. Hydroalcoholic Extract Against Standard and Clinical Strains of Escherichia Coli Compared with Conventional Antibiotics میکروبیولوژی Microbiology پژوهشي Research زمینه و هدف: عفونت‌های ادراری از شایع‌ترین بیماری‌های عفونی هستند که اغلب به واسطه باکتری اشریشیاکلی(Escherichia coli) ایجاد می‌شوند. افزایش مقاومت آنتی‌بیوتیکی این باکتری به دلیل مصرف گسترده آنتی‌بیوتیک‌ها و انتقال ژن‌های مقاومت، چالش‌های درمانی را تشدید کرده است. گیاه هلپه(Teucrium polium L.) به دلیل دارا بودن ترکیبات فعال زیستی، از جمله؛ مونوترپن‌ها و پلی‌فنول‌ها، در طب سنتی مورد توجه بوده است، لذا هدف از این مطالعه تعیین و بررسی آنتی‌باکتریال عصاره هیدروالکلی گیاه هلپه(Teucrium polium L.) بر سویه‌های استاندارد و بالینی اشریشیاکلی در مقایسه با آنتی‌بیوتیک‌های متداول بود. روش بررسی: این یک مطالعه تجربی می‌باشد که به وسیله عصاره هیدروالکلی گیاه هلپه با روش خیساندن از نمونه‌های جمع‌آوری شده در شهر یاسوج تهیه گردید. سویه استاندارد اشریشیاکلی(ATCC 25922) و سویه‌های بالینی جدا شده از بیماران مبتلا به عفونت ادراری بررسی شدند. حساسیت سویه‌ها به آنتی‌بیوتیک‌های نیتروفورانتوئین، جنتامایسین، کوتریماکسازول، سفیپیم، سفتریاکسون و سیپروفلوکساسین با روش دیسک دیفیوژن ارزیابی شد. اثر آنتی‌باکتریال عصاره با تعیین حداقل غلظت مهاری(MIC) و حداقل غلظت باکتری کشی(MBC) به روش رقیق سازی در میکروپلیت و اندازه‌گیری قطر هاله عدم رشد(ZOI) سنجیده شد. داده‌های جمع‌آوری شده با استفاده از آزمون‌های آماری کولموگروف ـ اسمیرنف، آنالیز واریانس یک طرفه، تی مستقل و آنالیز واریانس با اندازه گیری های مکرر تجزیه و تحلیل شدند. یافته‌ها: هر دو سویه استاندارد و بالینی اشریشیاکلی به آنتی‌بیوتیک‌های مورد استفاده حساس بودند. MIC عصاره هلپه برای سویه استاندارد 5/62 میکروگرم بر میلی‌لیتر و برای سویه‌های بالینی 25/31 تا 5/62 میکروگرم بر میلی‌لیتر بود. MBC در محدوده 125 تا250 میکروگرم بر میلی‌لیتر قرار داشت. تفاوت آماری معنی‌داری بین MIC و MBC سویه‌های استاندارد و بالینی مشاهده نشد(05/0p>). قطر هاله عدم رشد در سویه استاندارد بزرگ‌تر از سویه‌های بالینی بود، اما این تفاوت معنی‌دار نبود(05/0p>). عصاره گیاه هلپه اثر آنتی‌باکتریال قابل توجهی نشان داد، هر چند شدت آن نسبت به برخی آنتی‌بیوتیک‌ها کمتر بود. نتیجه‌گیری: عصاره هیدروالکلی گیاه هلپه پتانسیل آنتی‌باکتریال علیه اشریشیاکلی را دارد و می‌تواند به عنوان مکمل طبیعی در برابر مقاومت آنتی‌بیوتیکی مورد استفاده قرار گیرد.   1.Heidari SE, Heidari M, Doosti A. Epidemiology of urinary tract infection and antibiotic resistance pattern of E. coli in patients referred to Imam Ali hospital in Farokhshahr, Chaharmahal va Bakhtiari, Iran. 2013. 2.Moumeni L, Zamanizadeh B. The antibacterial properties of Allium cepa(onion) and Zingiber officinale(ginger) extracts on Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Candida albicans isolated from vaginal specimens. 2010. 3.Kumavath R, Gupta P, Tatta ER, Mohan M, Salim SA, Busi S. Unraveling the role of mobile genetic elements (MGEs) in antibiotic resistance transmission and defending strategies in Bacteria. Frontiers in Systems Biology 2025; 5: 1557413. 4.Ye J, Sun Q, Wu Q, Xu J, Yang Y, Zhao R, Li Q. The expression regulation of recA gene and bacterial class 2 integron-associated genes induced by antibiotics. Frontiers in Microbiology 2025; 16: 1632813. 5.Tajeddin E, Alebouyeh M, Mohammad Alizadeh AH, Zali MR. Relationship between antibiotic resistance patterns and existence of class 1, 2 and 3 integrons in bacterial isolates from human bile samples in patients with biliary tract disorders. 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Antimicrobial resistance pattern of Escherichia coli isolated from patients with urinary tract infection (UTI) in Yasuj city during 1391-1392. Armaghane Danesh 2014; 19(4): 337-46. 30.Molana Z, Shahandeh Z. Effect of garlic (allium sativum) and garlic extract on growth inhibition of pseudomonas aeruginosa. Journal of Babol University of Medical Sciences 2003; 5(3): 57-62. 31.Mahmoud M, Anahita DA, Parisa N, Somayeh A, Farzaneh N. Phytochemical study of two plants, chicory and colepore, and evaluation of their antimicrobial and antifungal effects, 2002. 32.Poursagh, Mashgholdzhiker A, Limoui M. Study of the therapeutic effects of the herb Teucrium polium in candidal infection in women in Sepidan city in 2011. Abstracts of the National Conference on Natural Products and Medicinal Plants. 2013;1(1). 33.Mohseni R, Hosseini M, Ghaedi M, Sharifi A, Mohammadi Hr, Moridikia F,et al. Investigation of antibacterial effect and expression of alkaline protease gene in pseudomonas aeruginosa (pao1) using copper oxide, copper complex and satureja khuzistanica: rt-pcr method. International Journal of Pharmaceutical Research 2020; 12(4): 09752366. 34.Teimori M. Investigation of antimicrobial effect of extract of Teucrium polium L. on some gram positive and gram negative bacteria. Biology Findings 2012; 8(1): 1-6. 35.Tabatabaei Yazdi F, Alizadeh Behbahani B, Heidari Sureshjani M, Mortazavi SA. The In vitro study of antimicrobial effect of teucrium polium extract on infectious microorganisms. Avicenna Journal of Clinical Medicine 2014; 21(1): 16-24. <v:rect id="_x0000_s1028" strokecolor="white" style="position:absolute; margin-left:1px; margin-top:-63px; width:229.9pt; height:20.9pt; z-index:251660288">Armaghane-danesh, Yasuj University of                                                                     Original Article <v:shape id="Text_x0020_Box_x0020_11" o:gfxdata="UEsDBBQABgAIAAAAIQC2gziS/gAAAOEBAAATAAAAW0NvbnRlbnRfVHlwZXNdLnhtbJSRQU7DMBBF 90jcwfIWJU67QAgl6YK0S0CoHGBkTxKLZGx5TGhvj5O2G0SRWNoz/78nu9wcxkFMGNg6quQqL6RA 0s5Y6ir5vt9lD1JwBDIwOMJKHpHlpr69KfdHjyxSmriSfYz+USnWPY7AufNIadK6MEJMx9ApD/oD OlTrorhX2lFEilmcO2RdNtjC5xDF9pCuTyYBB5bi6bQ4syoJ3g9WQ0ymaiLzg5KdCXlKLjvcW893 SUOqXwnz5DrgnHtJTxOsQfEKIT7DmDSUCaxw7Rqn8787ZsmRM9e2VmPeBN4uqYvTtW7jvijg9N/y JsXecLq0q+WD6m8AAAD//wMAUEsDBBQABgAIAAAAIQA4/SH/1gAAAJQBAAALAAAAX3JlbHMvLnJl bHOkkMFqwzAMhu+DvYPRfXGawxijTi+j0GvpHsDYimMaW0Yy2fr2M4PBMnrbUb/Q94l/f/hMi1qR JVI2sOt6UJgd+ZiDgffL8ekFlFSbvV0oo4EbChzGx4f9GRdb25HMsYhqlCwG5lrLq9biZkxWOiqY 22YiTra2kYMu1l1tQD30/bPm3wwYN0x18gb45AdQl1tp5j/sFB2T0FQ7R0nTNEV3j6o9feQzro1i OWA14Fm+Q8a1a8+Bvu/d/dMb2JY5uiPbhG/ktn4cqGU/er3pcvwCAAD//wMAUEsDBBQABgAIAAAA IQAD5V4gIwIAAFIEAAAOAAAAZHJzL2Uyb0RvYy54bWysVNuO2yAQfa/Uf0C8N3aiJM1acVbbbFNV 2l6k3X4AxjhGBYYCiZ1+fQfIpmn7tqofEMMMh5lzZry+HbUiR+G8BFPT6aSkRBgOrTT7mn572r1Z UeIDMy1TYERNT8LT283rV+vBVmIGPahWOIIgxleDrWkfgq2KwvNeaOYnYIVBZwdOs4Cm2xetYwOi a1XMynJZDOBa64AL7/H0PjvpJuF3neDhS9d5EYiqKeYW0urS2sS12KxZtXfM9pKf02AvyEIzafDR C9Q9C4wcnPwHSkvuwEMXJhx0AV0nuUg1YDXT8q9qHntmRaoFyfH2QpP/f7D88/GrI7JF7aaUGKZR oycxBvIORoJHyM9gfYVhjxYDw4jnGJtq9fYB+HdPDGx7ZvbizjkYesFazC/dLK6uZhwfQZrhE7T4 DjsESEBj53QkD+kgiI46nS7axFw4Hi5nq+WiRBdH33zxFsWPyRWser5tnQ8fBGgSNzV1qH1CZ8cH H3Loc0h8zIOS7U4qlQy3b7bKkSPDPtml74z+R5gyZKjpzWK2yAS8AELLgA2vpK7pqoxfbsFI23vT pnYMTKq8x+qUwSIjj5G6TGIYm/GsSwPtCRl1kBsbBxE3PbiflAzY1DX1Pw7MCUrUR4Oq3Ezn8zgF yUgkUuKuPc21hxmOUDUNlOTtNuTJOVgn9z2+lPvAwB0q2clEckw1Z3XOGxs3yXQesjgZ13aK+v0r 2PwCAAD//wMAUEsDBBQABgAIAAAAIQDdznAA4AAAAA4BAAAPAAAAZHJzL2Rvd25yZXYueG1sTI9B T4NAEIXvJv6HzZh4MXYXTImlLE3TaDy39eJtC1MgsrPAbgv11zuc9PbezMubb7LNZFtxxcE3jjRE CwUCqXBlQ5WGz+P78ysIHwyVpnWEGm7oYZPf32UmLd1Ie7weQiW4hHxqNNQhdKmUvqjRGr9wHRLv zm6wJrAdKlkOZuRy28pYqURa0xBfqE2HuxqL78PFanDj28067FX89PVjP3bbfn+Oe60fH6btGkTA KfyFYcZndMiZ6eQuVHrRsldRxFEWy2WUgJgjajXPTqyS+CUBmWfy/xv5LwAAAP//AwBQSwECLQAU AAYACAAAACEAtoM4kv4AAADhAQAAEwAAAAAAAAAAAAAAAAAAAAAAW0NvbnRlbnRfVHlwZXNdLnht bFBLAQItABQABgAIAAAAIQA4/SH/1gAAAJQBAAALAAAAAAAAAAAAAAAAAC8BAABfcmVscy8ucmVs c1BLAQItABQABgAIAAAAIQAD5V4gIwIAAFIEAAAOAAAAAAAAAAAAAAAAAC4CAABkcnMvZTJvRG9j LnhtbFBLAQItABQABgAIAAAAIQDdznAA4AAAAA4BAAAPAAAAAAAAAAAAAAAAAH0EAABkcnMvZG93 bnJldi54bWxQSwUGAAAAAAQABADzAAAAigUAAAAA " strokecolor="white" style="position:absolute; 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margin-left:67px; margin-top:1034px; width:495pt; height:36pt; z-index:251658240" type="#_x0000_t202"> <v:textbox> <table width="100%"> <tbody> <tr> <td> <div>1056</div> </td> </tr> </tbody> </table> </v:textbox> </v:shape> Medical Sciences Journal (YUMSJ)</v:rect> In Vitro Antibacterial Activity of Teucrium Polium L. Hydroalcoholic Extract Against Standard and Clinical Strains of Escherichia Coli Compared with Conventional Antibiotics Hedayat SJ1, Ebrahimi H1, Sharifi A2, Eilami O3, Saeedinejad Z4, Khosravani SM1, Khosravani SA2* 1Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran, 2Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran, 3 HIV/AIDS Research Center, Shiraz University of Medical Sciences, Shiraz, Iran, 4Department of Internal Medicine, Yasuj University of Medical Sciences, Yasuj, Iran Received Date: 13 Apr 2025     Accepted Date: 13 Oct 2025                 Abstract Background & aim:  Urinary tract infections (UTIs) are among the most prevalent infectious diseases, frequently caused by Escherichia coli. The escalating antibiotic resistance of this bacterium, driven by widespread antibiotic use and the transfer of resistance genes, has intensified therapeutic challenges. Teucrium polium L. rich in bioactive compounds such as monoterpenes and polyphenols, has been valued in traditional medicine. Therefore, the aim of this study was to determine and evaluate the antibacterial activity of the hydroalcoholic extract of Teucrium polium L. against standard and clinical strains of Escherichia coli in comparison with conventional antibiotics. Methods: This is an experimental study in which the hydroalcoholic extract of Teucrium polium L. was prepared by the maceration method from plant samples collected in the city of Yasuj. The standard E. coli strain (ATCC 25922) and clinical isolates from UTI patients were tested. The susceptibility of the strains to antibiotics, including nitrofurantoin, gentamicin, cotrimoxazole, cefepime, ceftriaxone, and ciprofloxacin, was assessed using the disk diffusion method. The antibacterial activity of the extract was evaluated by determining the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) via the microdilution method, and the zone of inhibition (ZOI) diameter. The collected data were analyzed using the Kolmogorov–Smirnov test, one-way ANOVA, independent t-test, and repeated measures analysis of variance. Results: Both standard and clinical E. coli strains were susceptible to the tested antibiotics. The MIC of the T. polium extract was 62.5 μg/mL for the standard strain and ranged from 31.25 to 62.5 μg/mL for clinical isolates. The MBC ranged from 125 to 250 μg/mL. No stastically significant differences were observed between the MIC and MBC of standard and clinical strains (pvalue>0.05). The ZOI diameter was larger for the standard strain compared to clinical isolates, though this difference was not statistically significant (P.value>0.05). The T. polium extract exhibited considerable antibacterial activity, albeit less potent than some antibiotics. Conclusions: The hydroalcoholic extract of T. polium demonstrates antibacterial potential against E. coli and could serve as a natural adjunct to address antibiotic resistance. Further studies are recommended to investigate its toxicity and identify active compounds. These findings underscore the significance of medicinal plants in developing novel therapeutic strategies.   عفونت‌های ادراری, گیاه هلپه, اشریشیاکلی, عصاره گیاهی, مقاومت دارویی Urinary Tract Infections, Teucrium polium, Escherichia coli, Plant Extracts, Drug Resistance 34 49 http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-2973-3&slc_lang=fa&sid=1 SJ Hedayat سیدجواد هدایت Msaadat48@gmail.com 100319475328460033863 100319475328460033863 No Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran, کمیته تحقیقات دانشجویی، دانشگاه علوم پزشکی یاسوج، یاسوج، ایران H Ebrahimi هادی ابراهیمی hadiebmed@gmail.com 100319475328460033864 100319475328460033864 No Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran, کمیته تحقیقات دانشجویی، دانشگاه علوم پزشکی یاسوج، یاسوج، ایران A Sharifi اصغر شریفی asgharsharifi@yahoo.com 100319475328460033865 100319475328460033865 No Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran مرکز تحقیقات سلولی و مولکولی، دانشگاه علوم پزشکی یاسوج، یاسوج، ایران O Eilami اورنگ ایلامی owrangeilami@yahoo.com 100319475328460033866 100319475328460033866 No HIV/AIDS Research Center, Shiraz University of Medical Sciences, Shiraz, Iran مرکز تحقیقات ایدز، دانشگاه علوم پزشکی شیراز، شیراز، ایران Z Saeedinejad ذاکر سعیدی نژاد 100319475328460033867 100319475328460033867 No Department of Internal Medicine, Yasuj University of Medical Sciences, Yasuj, Iran گروه داخلی، دانشگاه علوم پزشکی یاسوج، یاسوج، ایران SM Khosravani سیدمحمد خسروانی ua89shahrokh@gmail.com 100319475328460033868 100319475328460033868 No Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran, کمیته تحقیقات دانشجویی، دانشگاه علوم پزشکی یاسوج، یاسوج، ایران SA Khosravani سیدعبدالمجید خسروانی khosravani2us@yahoo.com 100319475328460033869 100319475328460033869 Yes Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran مرکز تحقیقات سلولی و مولکولی، دانشگاه علوم پزشکی یاسوج، یاسوج، ایران