Armaghane Danesh
ارمغان دانش
armaghanj
Medical Sciences
http://armaghanj.yums.ac.ir
1
admin
1728-6506
1728-6514
10.61882/armaghanj
en
jalali
1404
6
1
gregorian
2025
9
1
30
4
online
1
fulltext
fa
تأثیر اسانس برگ شمعدانی بر سطح بیان گلوتاتیون، مالون دی آلدئید، سوپراکسید دیسموتاز و 8ـ هیدروکسی دیاکسی گوانوزین دراستئوآرتریت القا شده در مفاصل زانو خرگوش
The Effect of Geranium Leaf Essential Oil on the Expression Levels of Glutathione, Malondialdehyde, Superoxide Dismutase and 8-Hydroxydeoxyguanosine in Osteoarthritis Induced in Rabbit Knee Joints
علوم آزمایشگاهی
Clinical laboratory
پژوهشي
Research
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">زمینه و هدف</span></span></span></b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">:</span></span></span></b> <span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">تولید بیش از حد رادیکالهای آزاد موجب آپوپتوز استئوکندروسیتها و تخریب ماتریکس خارج سلولی میشود که در نهایت به بروز نقایص استئوکندرال منجر میگردد. از آن جا که اسانس برگ شمعدانی عطری</span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black"> (Pelargonium graveolens)</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">دارای ترکیبات آنتیاکسیدانی است، این مطالعه با هدف تعیین و تأثیر </span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">اسانس برگ شمعدانی بر سطح بیان </span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">گلوتاتیون</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">، مالون دی آلدئید، </span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">سوپراکسید دیسموتاز </span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">و </span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">8ـ هیدروکسی دیاکسی گوانوزین </span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">دراستئوآرتریت القا شده در مفاصل زانو خرگوش بود.</span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="color:black"></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"></span></span></span></b></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">روش بررسی</span></span></span></b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">:</span></span></span></b> <span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">این یک مطالعه تجربی میباشد که در سال </span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">۱۴۰۰</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> بر روی </span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">۳۰</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> سر خرگوش نژاد نیوزیلندی انجام گرفت. </span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">پس از تهیه اسانس گیاه، خرگوشها به مدت 1 ماه برای تطبیق با شرایط آزمایشگاهی نگهداری شدند. به زانوهای راست خرگوشها کلاژناز به صورت داخل مفصلی تزریق شد. 30 روز پس از ایجاد </span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">استئوآرتریت ناشی از کلاژناز، برای خرگوشها به صورت خوراکی با آب مقطر(کنترل مثبت )، اسانس برگ شمعدانی عطری 50 و ۱00 میلی گرم بر کیلوگرم و سلکوکسیب(4/0 درصد در 3/0 میلیلیتر) یک بار در روز به مدت ۳۰ روز تجویز شدند. </span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">در پایان هفتههای چهارم، هشتم و دوازدهم، سطوح </span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black">GSH</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">، </span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black">MDA</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">، </span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black">SOD</span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">و 8</span></span></span><b><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black">-</span></span></span></b><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black">OHdG</span></span></span><b> </b> <span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">در خون محیطی و مایع سینوویال اندازهگیری شدند. </span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">دادههای جمعآوری شده با استفاده از آزمونهای آماری تحلیل واریانس یک طرفه، توکی و تی تجزیه و تحیلی شدند. </span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">یافتهها</span></span></span></b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">:</span></span></span></b><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> نتایج نشان داد که در گروه تیمار با اسانس شمعدانی، سطوح</span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black"> GSH </span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">و</span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black"> SOD </span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">در هفتههای هشتم و دوازدهم به طور معنیداری بالاتر از گروه کنترل بود(05/0</span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black">p<</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">). </span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">همچنین بیان</span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black"> MDA </span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">و 8</span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black">-OHdG </span></span></span><span style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> در گروه کنترل مثبت روند افزایشی داشت، در حالیکه در گروه تیمار کاهش قابل توجهی مشاهده شد(05/0</span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black">p<</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">)</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">.</span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black"></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">نتیجهگیری: </span></span></span></b><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">اسانس برگ شمعدانی عطری با افزایش فعالیت آنتیاکسیدانی، افزایش بیان</span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black"> GSH </span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">و</span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black"> SOD </span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">و کاهش بیان شاخصهای استرس اکسیداتیو</span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black"> MDA </span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">و 8</span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black">-OHdG) </span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">میتواند نقش حفاظتی در برابر آسیبهای استئوکندرال ناشی از استرس اکسیداتیو ایفا کند.</span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black"></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"></span></span></span></b></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:10%"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="fa" style="font-size:10.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black">Background & aim:</span></span></span></b><b> </b><span style="font-size:10.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black">Excessive production of free radicals induces apoptosis of osteochondral cells and degradation of the extracellular matrix, ultimately leading to osteochondral defects. Given that the essential oil of <i>Pelargonium graveolens</i> (rose geranium) contains antioxidant constituents, the present study aimed to determine the effects of rose-geranium leaf essential oil on the expression levels of glutathione (GSH), malondialdehyde (MDA), superoxide dismutase (SOD), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) in collagenase-induced osteoarthritis of the rabbit knee joint.</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:10%"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span style="font-size:6.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black"></span></span></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:10%"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Arial",sans-serif"><span style="color:black">Methods: </span></span></b><span style="font-family:"Arial",sans-serif"><span style="color:black">This experimental study was conducted in 2021 on 30 New Zealand white rabbits. After preparation of the plant essential oil, the rabbits were acclimatized to laboratory conditions for one month. Osteoarthritis was induced by intra-articular injection of collagenase into the right knee joints. Thirty days after OA induction, the rabbits received oral treatments of distilled water (positive control), rose-geranium leaf essential oil at 50 or 100 mg/kg, or celecoxib (0.3 mL of 0.4%), once daily for 30 days. At the end of weeks 4, 8, and 12, levels of GSH, MDA, SOD, and 8-OHdG were measured in peripheral blood and synovial fluid. Data were analyzed using one-way ANOVA, Tukey’s post hoc test, and t-tests.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:10%"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span style="font-size:4.5pt"><span style="font-family:"Arial",sans-serif"><span style="color:black"></span></span></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:10%"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><a name="_Hlk219144817"><b><span style="font-size:9.5pt"><span style="font-family:"Arial",sans-serif"><span style="color:black">Results:</span></span></span></b></a> <span style="font-family:"Arial",sans-serif"><span style="color:black">In the essential-oil–treated group, GSH and SOD levels at weeks 8 and 12 were significantly higher than in the control group (p<0.05). Conversely, MDA and 8-OHdG levels indicated an increasing trend in the positive control group, whereas a significant reduction was observed in the treated groups (p<0.05).</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:10%"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span style="font-size:6.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black"></span></span></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:10%"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Arial",sans-serif"><span style="color:black">Conclusion</span></span></b><span style="font-family:"Arial",sans-serif"><span style="color:black">: Rose-geranium leaf essential oil enhanced antioxidant activity by increasing GSH and SOD expression and reducing oxidative-stress markers (MDA and 8-OHdG). These findings suggest a potential protective role of this essential oil against oxidative stress–induced osteochondral damage.</span></span></span></span></span></span></span><br>
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اسانس برگ شمعدانی عطری, مالون دی آلدئید, سوپراکسید دیسموتاز, 8-هیدروکسی دی اکسی گوانوزین, گلوتاتیون
Geranium essential oil, Osteoarthritis, glutathione (GSH), Malondialdehyde (MDA), Superoxide dismutase(SOD), 8-hydroxy-2′-deoxyguanosine (8-OHdG)
466
484
http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-2508-2&slc_lang=fa&sid=1
H
Maghsoudi
حسین
مقصودی
hossein_m2002@yahoo.com
0026429179
100319475328460032957
Yes
Department of Biotechnology, Payam Noor University of Tehran, Tehran, Iran
گروه بیوتکنولوژی دانشگاه پیام نور تهران، تهران، ایران
F
Jola
فرشته
جولا
f.j.rozikhosj@gmail.com
100319475328460032958
100319475328460032958
No
Department of Biology, Payam Noor University of Tehran, Iran
گروه بیولوژی دانشگاه پیام نور تهران، تهران، ایران
GH
Bakhshi Khaneghi
غلامرضا
بخشی خانگی
bakhshi@pnu.ac.ir
100319475328460032959
100319475328460032959
No
Department of Biology, Payam Noor University of Tehran, Iran
گروه بیولوژی دانشگاه پیام نور تهران، تهران، ایران