Armaghane Danesh
ارمغان دانش
armaghanj
Medical Sciences
http://armaghanj.yums.ac.ir
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gregorian
2026
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تأثیر عصاره الکلی میوهPistacia atlantica (بنه) بر روی ایزوله‌های بالینی کاندیدا آلبیکنس جدا شده از مبتلایان به دنچر استوماتیت در مقایسه با نیستاتین
The Effect of Hydroalcoholic Extract of Pistacia atlantica Fruit (Bene) on Clinical Isolates of Candida albicans from Patients with Denture Stomatitis Compared to Nystatin
دندانپزشکی
Dentistry
كاربردي
Applicable
<span style="font-size:11pt"><span style="background:white"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"yaghut",serif"><b><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">زمینه و هدف:</span></span></span></b><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> دنچر استوماتیت شایع‌ترین عفونت قارچی دهانی در سالمندان است که عمدتاً به وسیله کاندیدا آلبیکنس ایجاد می‌شود، لذا هدف از این مطالعه تعیین و بررسی فعالیت ضدقارچی عصاره هیدروالکلی میوه درخت بنه(</span></span></span><i><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black">Pistacia atlantica</span></span></span></i><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">) در مقایسه با نیستاتین علیه ایزوله‌های بالینی کاندیدا آلبیکنس جدا‌شده از بیماران مبتلا به دنچر استوماتیت بود.</span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black"></span></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="background:white"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"yaghut",serif"><b><span lang="AR-SA" style="font-size:8.0pt"><span style="font-family:Yagut"><span style="color:black"></span></span></span></b></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="background:white"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"yaghut",serif"><b><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">روش بررسی:</span></span></span></b> <span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">این یک مطالعه توصیفی ـ آزمایشگاهی در سال </span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">۱۴۰۲</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> انجام شد و از </span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">۱۹</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> بیمار سالمند مبتلا به دنچر استوماتیت نمونه‌برداری شد.</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> ایزوله‌های</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> کاندیدا با کشت روی سابورو دکستروز آگار، کروم‌آگار کاندیدا، تست جرم تیوب و رشد در </span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">۴۲</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> درجه سانتی‌گراد شناسایی و تأیید شدند. در نهایت </span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">۱۰</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> ایزوله کاندیدا آلبیکنس به دست آمد. عصاره هیدروالکلی میوه بنه با روش ماسراسیون تهیه و حساسیت‌سنجی به روش براث میکرودیلوشن(</span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black">CLSI M27-S4</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">) در محیط</span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black">RPMI </span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">۱۶۴۰</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> در سه تکرار انجام گرفت. داده‌های جمع‌آوری شده با استفاده از آزمون‌های آماری </span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">شاپرویلک، نان پارامتریک و </span></span></span><span lang="AR-SA" style="font-family:Yagut"><span style="color:black">ویلکاکسون</span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> تجزیه و تحلیل شدند. </span></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="background:white"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"yaghut",serif"><span dir="LTR" lang="AR-SA" style="font-size:6.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black"></span></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="background:white"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"yaghut",serif"><b><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">یافته‌ها:</span></span></span></b><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> دامنه </span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black">MIC</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> عصاره بنه </span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">2ـ1</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> میلی‌گرم بر میلی‌لیتر، </span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black">MIC</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">۵۰ </span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">،</span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">۱</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> و </span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black">MIC</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">۹۰ </span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">برابر با </span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">۲</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> میلی‌گرم بر میلی‌لیتر بود. دامنه </span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black">MIC</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> نیستاتین</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">125/0</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> تا بیش از </span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">۴</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> میکروگرم بر میلی‌لیتر، </span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black">MIC</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">۵۰ </span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">،</span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">125/0</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> و </span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black">MIC</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">۹۰ </span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">،</span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">۲</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> میکروگرم بر میلی‌لیتر بود. تفاوت بین </span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black">MIC</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> دو ترکیب از نظر آماری معنی‌دار بود(آزمون ویلکاکسون 004/0=</span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black">p</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">).</span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black"></span></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="background:white"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"yaghut",serif"><b><span lang="AR-SA" style="font-size:7.0pt"><span style="font-family:Yagut"><span style="color:black"></span></span></span></b></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="background:white"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"yaghut",serif"><b><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">نتیجه‌گیری:</span></span></span></b><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> عصاره هیدروالکلی میوه درخت بنه فعالیت ضدقارچی قابل‌توجهی علیه ایزوله‌های بالینی کاندیدا آلبیکنس دارد، هرچند به‌طور معنی‌داری ضعیف‌تر از نیستاتین عمل می‌کند. این عصاره می‌تواند به‌عنوان مکمل یا پایه‌ای برای توسعه فرآورده‌های گیاهی ضدقارچی موضعی مورد بررسی بیشتر قرار گیرد.</span></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="background:white"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"yaghut",serif"><span dir="LTR" style="font-size:3.0pt"><span style="font-family:"Arial",sans-serif"><span style="color:black"></span></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="background:white"><span style="line-height:115%"><span style="unicode-bidi:embed"><span style="font-family:"yaghut",serif"><b><span lang="fa" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"Arial",sans-serif"><span style="color:black">Background & aim:</span></span></span></span></b><b> </b><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"Arial",sans-serif"><span style="color:black">Denture stomatitis is the most common oral fungal infection in the elderly, primarily caused by <i>Candida albicans</i>. The present study aimed to investigate the antifungal activity of the hydroalcoholic extract of <i>Pistacia atlantica</i> fruit (bene) compared to nystatin against clinical isolates of <i>Candida albicans</i> obtained from patients with denture stomatitis.</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="background:white"><span style="line-height:115%"><span style="unicode-bidi:embed"><span style="font-family:"yaghut",serif"><span style="font-size:1.0pt"><span style="line-height:115%"><span style="font-family:"Arial",sans-serif"><span style="color:black"></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="background:white"><span style="line-height:115%"><span style="unicode-bidi:embed"><span style="font-family:"yaghut",serif"><b><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"Arial",sans-serif"><span style="color:black">Methods:</span></span></span></span></b><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"Arial",sans-serif"><span style="color:black"> The present in vitro descriptive-analytical study was conducted in 2023. Samples were collected from 19 elderly patients with denture stomatitis. <i>Candida</i> isolates were identified and confirmed by culture on Sabouraud dextrose agar, CHROMagar <i>Candida</i>, germ tube test, and growth at 42°C. Ultimately, 10 isolates of <i>Candida albicans</i> were obtained. The hydroalcoholic extract of bene fruit was prepared using the maceration method, and susceptibility testing was performed by the broth microdilution method (CLSI M27-S4) in RPMI 1640 medium in triplicate. Data were analyzed using Shapiro-Wilk, Friedman, and Wilcoxon tests via SPSS software version 20.</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="background:white"><span style="line-height:115%"><span style="unicode-bidi:embed"><span style="font-family:"yaghut",serif"><span style="font-size:1.0pt"><span style="line-height:115%"><span style="font-family:"Arial",sans-serif"><span style="color:black"></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="background:white"><span style="line-height:115%"><span style="unicode-bidi:embed"><span style="font-family:"yaghut",serif"><b><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"Arial",sans-serif"><span style="color:black">Results:</span></span></span></span></b><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"Arial",sans-serif"><span style="color:black"> The MIC range for the bene extract was 1–2 mg/mL, with MIC</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"Cambria Math",serif"><span style="color:black">₅₀</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"Arial",sans-serif"><span style="color:black"> of 1 mg/mL and MIC</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"Cambria Math",serif"><span style="color:black">₉₀</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"Arial",sans-serif"><span style="color:black"> of 2 mg/mL. The MIC range for nystatin was 0.125 to more than 4 μg/mL, with MIC</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"Cambria Math",serif"><span style="color:black">₅₀</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"Arial",sans-serif"><span style="color:black"> of 0.125 μg/mL and MIC</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"Cambria Math",serif"><span style="color:black">₉₀</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"Arial",sans-serif"><span style="color:black"> of 2 μg/mL. The difference in MIC between the two compounds was statistically significant (Wilcoxon test, p=0.004).</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="background:white"><span style="line-height:115%"><span style="unicode-bidi:embed"><span style="font-family:"yaghut",serif"><span style="font-size:2.0pt"><span style="line-height:115%"><span style="font-family:"Arial",sans-serif"><span style="color:black"></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="background:white"><span style="line-height:115%"><span style="unicode-bidi:embed"><span style="font-family:"yaghut",serif"><b><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"Arial",sans-serif"><span style="color:black">Conclusion:</span></span></span></span></b><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"Arial",sans-serif"><span style="color:black"> The hydroalcoholic extract of <i>Pistacia atlantica</i> fruit exhibits notable antifungal activity against clinical isolates of <i>Candida albicans</i>, although it is significantly less potent than nystatin. This extract could be considered as a supplement or a basis for further investigation in the development of topical herbal antifungal pr</span></span></span></span></span></span></span></span></span>
استوماتیت, دنچر, کاندیدا آلبیکنس, عصاره‌های گیاهی, نیستاتین
Stomatitis, Denture, Candida albicans, Plant extracts, Nystatin
212
224
http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-3068-1&slc_lang=fa&sid=1
R
Baziar
رضا
بازیار
reza.bazyar@gmail.com
100319475328460034197
100319475328460034197
No
Department of Dentistry, Yasuj University of Medical Sciences, Yasuj, Iran,
گروه دندانپزشکی، دانشگاه علوم پزشکی یاسوج، یاسوج، ایران
F
Khajehzadeh
فاطمه
خواجه زاده
khajezadehfatemeh3@gmail.com
100319475328460034196
100319475328460034196
Yes
Department of Oral and Maxillofacial Pathology, Yasuj University of Medical Sciences, Yasuj, Iran
گروه پاتولوژی دهان و فک و صورت، دانشگاه علوم پزشکی یاسوج، یاسوج، ایران،
M
Ghorghani
مارال
قرقانی
maralgharaghani@gmail.com
100319475328460034198
100319475328460034198
No
Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
مرکز تحقیقات گیاهان دارویی، دانشگاه علوم پزشکی یاسوج، یاسوج، ایران
E
Panahi Kokhdan
اسماعیل
پناهی کوخدان
esmalpanahi@gmail.com
100319475328460034199
100319475328460034199
No
Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
مرکز تحقیقات گیاهان دارویی، دانشگاه علوم پزشکی یاسوج، یاسوج، ایران
H
Bardania
حسن
بردانیا
h.bardania@gmail.com
100319475328460034200
100319475328460034200
No
Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
مرکز تحقیقات سلولی و ملکولی، دانشگاه علوم پزشکی یاسوج، یاسوج، ایران