Armaghane Danesh
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armaghanj
Medical Sciences
http://armaghanj.yums.ac.ir
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2026
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تاثیر عصاره الکلی میوه Pistacia atlantica (بنه) بر روی ایزوله های بالینی کاندیدا آلبیکنس جدا شده از مبتلایان به دنچر استوماتیت در مقایسه با نیستاتین
The Effect of Hydroalcoholic Extract of Pistacia atlantica Fruit (Bene) on Clinical Isolates of Candida albicans from Patients with Denture Stomatitis Compared to Nystatin
دندانپزشکی
Dentistry
كاربردي
Applicable
<br>
<span style="font-size:11pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:yaghut,serif"><b><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif">زمینه و هدف:</span></span></b><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif"> دنچر استوماتیت شایعترین عفونت قارچی دهانی در سالمندان است که عمدتاً توسط کاندیدا آلبیکنس ایجاد میشود. هدف از این مطالعه بررسی فعالیت ضدقارچی عصاره هیدروالکلی میوه درخت بنه (</span></span><span dir="LTR" style="background:lime"><span style="font-family:"Times New Roman",serif">Pistacia atlantica</span></span><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif">) در مقایسه با نیستاتین علیه ایزولههای بالینی کاندیدا آلبیکنس جداشده از بیماران مبتلا به دنچر استوماتیت بود.</span></span><span dir="LTR" style="background:lime"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:yaghut,serif"><b><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif">مواد و روشها:</span></span></b><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif"> از </span></span><span lang="FA" style="background:lime"><span style="font-family:"Times New Roman",serif">۱۹</span></span><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif"> بیمار سالمند مبتلا به دنچر استوماتیت نمونهبرداری شد. ایزولههای کاندیدا با کشت روی سابورو دکستروز آگار، کرومآگار کاندیدا، تست جرم تیوب و رشد در </span></span><span lang="FA" style="background:lime"><span style="font-family:"Times New Roman",serif">۴۲</span></span><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif"> درجه سانتیگراد شناسایی و تأیید شدند. در نهایت </span></span><span lang="FA" style="background:lime"><span style="font-family:"Times New Roman",serif">۱۰</span></span><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif"> ایزوله کاندیدا آلبیکنس بهدست آمد. عصاره هیدروالکلی میوه بنه با روش ماسراسیون تهیه و حساسیتسنجی به روش براث میکرودیلوشن (</span></span><span dir="LTR" style="background:lime"><span style="font-family:"Times New Roman",serif">CLSI M27-S4</span></span><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif">) در محیط </span></span><span dir="LTR" style="background:lime"><span style="font-family:"Times New Roman",serif">RPMI </span></span><span lang="FA" style="background:lime"><span style="font-family:"Times New Roman",serif">۱۶۴۰</span></span><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif"> در سه تکرار انجام گرفت. </span></span><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif">به منظور مقایسه میزان MIC ترکیب مورد مطالعه با داروی نیستاتین از نرم افزار SPSS V20 استفاده گردید. بدین منظور، نرمالیتی داده ها با آزمون شاپرویلک مورد ارزیابی قرار گرفت و سپس از آزمون نان پارامتریک برای مقایسه بین گروه ها استفاده گردید. </span></span><span dir="LTR" lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:yaghut,serif"><b><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif">یافتهها:</span></span></b><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif"> دامنه </span></span><span dir="LTR" style="background:lime"><span style="font-family:"Times New Roman",serif">MIC</span></span><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif"> عصاره بنه </span></span><span lang="FA" style="background:lime"><span style="font-family:"Times New Roman",serif">۱–۲</span></span><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif"> میلیگرم بر میلیلیتر، </span></span><span dir="LTR" style="background:lime"><span style="font-family:"Times New Roman",serif">MIC</span></span><span lang="FA" style="background:lime"><span style="font-family:"Times New Roman",serif">۵۰ </span></span><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif">،</span></span> <span lang="FA" style="background:lime"><span style="font-family:"Times New Roman",serif">۱</span></span><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif"> و </span></span><span dir="LTR" style="background:lime"><span style="font-family:"Times New Roman",serif">MIC</span></span><span lang="FA" style="background:lime"><span style="font-family:"Times New Roman",serif">۹۰ </span></span><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif">برابر با </span></span> <span lang="FA" style="background:lime"><span style="font-family:"Times New Roman",serif">۲</span></span><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif"> میلیگرم بر میلیلیتر بود. دامنه </span></span><span dir="LTR" style="background:lime"><span style="font-family:"Times New Roman",serif">MIC</span></span><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif"> نیستاتین </span></span><span lang="FA" style="background:lime"><span style="font-family:"Times New Roman",serif">۰</span></span><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif">٫</span></span><span lang="FA" style="background:lime"><span style="font-family:"Times New Roman",serif">۱۲۵</span></span><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif"> تا بیش از </span></span><span lang="FA" style="background:lime"><span style="font-family:"Times New Roman",serif">۴</span></span><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif"> میکروگرم بر میلیلیتر، </span></span><span dir="LTR" style="background:lime"><span style="font-family:"Times New Roman",serif">MIC</span></span><span lang="FA" style="background:lime"><span style="font-family:"Times New Roman",serif">۵۰ </span></span><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif">،</span></span> <span lang="FA" style="background:lime"><span style="font-family:"Times New Roman",serif">۰</span></span><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif">٫</span></span><span lang="FA" style="background:lime"><span style="font-family:"Times New Roman",serif">۱۲۵</span></span><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif"> و </span></span><span dir="LTR" style="background:lime"><span style="font-family:"Times New Roman",serif">MIC</span></span><span lang="FA" style="background:lime"><span style="font-family:"Times New Roman",serif">۹۰ </span></span><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif">،</span></span> <span lang="FA" style="background:lime"><span style="font-family:"Times New Roman",serif">۲</span></span><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif"> میکروگرم بر میلیلیتر بود. تفاوت بین </span></span><span dir="LTR" style="background:lime"><span style="font-family:"Times New Roman",serif">MIC</span></span><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif"> دو ترکیب از نظر آماری معنیدار بود (آزمون ویلکاکسون، </span></span><span dir="LTR" style="background:lime"><span style="font-family:"Times New Roman",serif">p=</span></span><span lang="FA" style="background:lime"><span style="font-family:"Times New Roman",serif">۰</span></span><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif">٫</span></span><span lang="FA" style="background:lime"><span style="font-family:"Times New Roman",serif">۰۰۴).</span></span><span dir="LTR" style="background:lime"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:yaghut,serif"><b><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif">نتیجهگیری:</span></span></b><span lang="AR-SA" style="background:lime"><span style="font-family:"Times New Roman",serif"> عصاره هیدروالکلی میوه درخت بنه فعالیت ضدقارچی قابلتوجهی علیه ایزولههای بالینی کاندیدا آلبیکنس دارد، هرچند بهطور معنیداری ضعیفتر از نیستاتین عمل میکند. این عصاره میتواند بهعنوان مکمل یا پایهای برای</span></span><span lang="AR-SA" style="font-family:"Times New Roman",serif"> <span style="background:lime">توسعه فرآوردههای گیاهی ضدقارچی موضعی مورد بررسی بیشتر قرار گیرد.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:yaghut,serif"><span lang="FA" style="font-family:"Times New Roman",serif"></span></span></span></span></span></span>
<p dir="auto" node="[object Object]"><strong node="[object Object]">Background and Objective:</strong> Denture stomatitis is the most common oral fungal infection in the elderly, primarily caused by Candida albicans. The aim of this study was to investigate the antifungal activity of the hydroalcoholic extract of Pistacia atlantica fruit (bene) compared to nystatin against clinical isolates of Candida albicans obtained from patients with denture stomatitis.</p>
<p dir="auto" node="[object Object]"><strong node="[object Object]">Materials and Methods:</strong> Samples were collected from 19 elderly patients with denture stomatitis. Candida isolates were identified and confirmed by culture on Sabouraud dextrose agar, CHROMagar Candida, germ tube test, and growth at 42°C. Ultimately, 10 isolates of Candida albicans were obtained. The hydroalcoholic extract of bene fruit was prepared using the maceration method, and susceptibility testing was performed by the broth microdilution method (CLSI M27-S4) in RPMI 1640 medium in triplicate. To compare the minimum inhibitory concentration (MIC) of the studied compound with nystatin, SPSS software version 20 was used. Data normality was assessed using the Shapiro-Wilk test, followed by a non-parametric test for inter-group comparisons.</p>
<p dir="auto" node="[object Object]"><strong node="[object Object]">Results:</strong> The MIC range for the bene extract was 1–2 mg/mL, with MIC₅₀ of 1 mg/mL and MIC₉₀ of 2 mg/mL. The MIC range for nystatin was 0.125 to more than 4 μg/mL, with MIC₅₀ of 0.125 μg/mL and MIC₉₀ of 2 μg/mL. The difference in MIC between the two compounds was statistically significant (Wilcoxon test, p=0.004).</p>
<p dir="auto" node="[object Object]"><strong node="[object Object]">Conclusion:</strong> The hydroalcoholic extract of Pistacia atlantica fruit exhibits notable antifungal activity against clinical isolates of Candida albicans, although it is significantly less potent than nystatin. This extract could be considered as a supplement or a basis for further investigation in the development of topical herbal antifungal products.</p>
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استوماتیت، دنچر، کاندیدا آلبیکنس، عصارههای گیاهی، نیستاتین
Stomatitis, Denture, Candida albicans, Plant Extracts, Nystatin
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http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-3068-1&slc_lang=fa&sid=1
FATEMEH
KHAJEHZADEH
فاطمه
خواجه زاده
khajezadehfatemeh3@gmail.com
100319475328460032800
100319475328460032800
Yes
REZA
BAZYAR
رضا
بازیار
reza.bazyar@gmail.com
100319475328460032801
100319475328460032801
No
MARAL
GHARGHANI
مارال
قرقانی
maralgharaghani@gmail.com
100319475328460032802
100319475328460032802
No
ESMAEIL
PANAHI
اسماعیل
پناهی کوخدان
esmalpanahi@gmail.com
100319475328460032803
100319475328460032803
No
HASAN
BARDANIA
حسن
بردانیا
h.bardania@gmail.com
100319475328460032804
100319475328460032804
No