Armaghane Danesh
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armaghanj
Medical Sciences
http://armaghanj.yums.ac.ir
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ارزیابی تغییرات هیستوپاتولوژیک سم تریسیکلازول بر بافت مغز در موش صحرایی نژاد ویستار
Evaluation of histopathological changes of tricyclazole toxin on brain tissue iaEvaluation of Histopathological Changes of Tricyclazole Toxin on Brain Tissue in Wistar Rats
s
عمومى
General
پژوهشي
Research
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">زمینه و هدف:</span></span></span></b><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> منابع غذایی انسان همواره به وسیله عوامل آسیبرسان بیشماری از جمله بقایای سموم و آفتکشهای مورد استفاده در کشاورزی تهدید میشوند، که در این میان قارچکش سیستمیک تریسیکلازول</span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial","sans-serif""><span style="color:black">(TCZ)</span></span></span><span style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> به علت اثرات سیتوتوکسیک، بسیار مورد توجه قرار گرفته است. بنابر این هدف از این مطالعه </span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">تعیین و </span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">ارزیابی تغییرات هیستوپاتولوژیک سم تریسیکلازول بر بافت مغز در موش صحرایی نژاد ویستار بود.</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"></span></span></span></b></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">روش بررسی:</span></span></span></b> <span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">در این مطالعه تجربی که در سال1401 به مدت 28 روز به منظور بررسی کیفی ضایعات پاتولوژیکی و شمارش کمی نورونهای مغزی انجام شد، 32 سر موش صحرایی نر نژاد ویستار وارد مطالعه شدند و در چهار گروه هشتتایی شامل؛ کنترل، دریافتکننده سم به صورت خوراکی با دوزهای</span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial","sans-serif""><span style="color:black">(A)</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">50، </span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial","sans-serif""><span style="color:black">(B)</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">5/37 و</span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial","sans-serif""><span style="color:black">(C)</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">25 (میلیگرم بر کیلوگرم وزن بدن) استفاده و پس از نمونهبرداری</span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">از بافت مغز، لامها با رنگآمیزی نیسل و هماتوکسیلین ـ ائوزین بررسی شدند. دادههای جمعآوری شده با استفاده از آزمونهای آماری</span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">کلموگروف ـ اسمیرنف،</span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">آنالیز واریانس یکطرفه و آزمون مقایسه چندگانه توکی تجزیه و تحلیل شدند.</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">یافتهها:</span></span></span></b><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> بررسیهای صورت گرفته در گروههای دریافت کننده سم، ضایعات پاتولوژیکی از جمله نکروزه شدن نورونها و افزایش سلولهای میکروگلیا در ناحیه هیپوکامپ و قشر مغز را نشان داد که متعاقب آن نتایج شمارش سلولی مشخص کننده کاهش معنیدار تعداد نورونها در ناحیه قشر مغز </span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial","sans-serif""><span style="color:black">A</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">(68/4±20/157)، </span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial","sans-serif""><span style="color:black">B</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">(94/4±20/167) و </span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial","sans-serif""><span style="color:black">C</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">(46/5±60/186) و نواحی مختلف هیپوکامپ(</span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial","sans-serif""><span style="color:black">DG</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">، </span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial","sans-serif""><span style="color:black">(CA1-4</span></span></span><span style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> در تمامی گروهها نسبت به گروه کنترل بود(05/0</span></span></span><span dir="LTR" style="font-size:8.0pt"><span style="font-family:"Arial","sans-serif""><span style="color:black">p<</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">).</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"></span></span></span></b></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">نتیجهگیری:</span></span></span></b> <span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">نتایج پژوهش نشان داد که ترکیب ضد قارچ تری سیکلازول باعث آسیب و مرگ پاتولوژیک نورونها در قشر مغز و نواحی مختلف هیپوکامپ و کاهش تعداد سلولهای این نواحی میشود و شدت ضایعات با افزایش دوز رابطه مستقیم دارد.</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="font-family:"Arial","sans-serif""><span style="color:black">Background & aim:</span></span></span></b><span style="font-size:10.0pt"><span style="font-family:"Arial","sans-serif""><span style="color:black"> Human food sources are always threatened by countless harmful factors, including residues of toxins and pesticides used in agriculture; in the meantime, the systemic fungicide Tricyclazole (TCZ) has received much attention due to its cytotoxic effects.Hence, the purpose of the present study was to determine and evaluate the histopathological changes of tricyclazole toxin on brain tissue in Wistar rats.</span></span></span><span style="font-size:10.0pt"><span style="font-family:"Arial","sans-serif""><span style="color:black"></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="font-family:"Arial","sans-serif""><span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span></span></b></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="font-family:"Arial","sans-serif""><span style="color:black"><span style="letter-spacing:-.2pt">Methods</span></span></span></span></b><b><span style="font-size:10.0pt"><span style="font-family:"Arial","sans-serif""><span style="color:black">:</span></span></span></b><span style="font-size:10.0pt"><span style="font-family:"Arial","sans-serif""><span style="color:black"> The present experimental study conducted in 2022 which lasted for 28 days to qualitatively examine pathological lesions and quantitatively count brain neurons, 32 male Wistar rats were divided into four groups of eight, including: a control group,and groups receiving toxin orally at doses of (A) 50, (B) 37.5, and (C) 25 (mg/kg body weight). After sampling the brain tissue, the slides were examined with Nissl and hematoxylin-eosin staining and then the data were analyzed using SPSS-26 statistical software, followed by one-way analysis of variance and Tukey's multiple comparison test.</span></span></span><span style="font-size:10.0pt"><span style="font-family:"Arial","sans-serif""><span style="color:black"></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="font-family:"Arial","sans-serif""><span style="color:black"></span></span></span></b></span></span></span><br>
<span style="font-size:12pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="font-family:"Arial","sans-serif""><span style="color:black">Results: </span></span></span></b><span style="font-size:10.0pt"><span style="font-family:"Arial","sans-serif""><span style="color:black">Investigations carried out in the groups receiving the poison indicated pathological lesions, including necrosis of neurons and an increase in microglia cells in the hippocampus and cerebral cortex, followed by the results of cell counting indicating a significant decrease in the number of neurons in the cerebral cortex A (157/20 ± 4/68), B(167/20 ± 4/94) and C (186/60 ± 5/46) and different hippocampus areas (CA1-4, DG) in all groups compared to the control group (p<0.05).</span></span></span><span style="font-size:10.0pt"><span style="font-family:"Arial","sans-serif""><span style="color:black"></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="tab-stops:236.25pt"><span style="vertical-align:top"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="font-family:"Arial","sans-serif""><span style="color:black"></span></span></span></b></span></span></span></span><br>
<span style="font-size:12pt"><span style="tab-stops:236.25pt"><span style="vertical-align:top"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="font-family:"Arial","sans-serif""><span style="color:black">Conclusion:</span></span></span></b><span style="font-size:10.0pt"><span style="font-family:"Arial","sans-serif""><span style="color:black"> The results of the present study revealed that the antifungal combination of tricyclazole caused damage and pathological death of neurons in the cerebral cortex and different areas of the hippocampus and decreased the number of cells in these areas; that the severity of the lesions was directly related to increasing the dose.</span></span></span><i> </i></span></span></span></span><br>
<span style="font-size:12pt"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:10.0pt"><span style="font-family:"Arial","sans-serif""><span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span></span></b></span></span><br>
<span style="font-size:12pt"><span style="font-family:"Times New Roman","serif""><span style="font-size:10.0pt"><span style="font-family:"Arial","sans-serif""><span style="color:black"></span></span></span></span></span>
تری سیکلازول, سمیت عصبی, هیستوپاتولوژی, قارچکش, موشصحرایی
Tricyclazole, Neurotoxicity, histopathology, fungicide, Rat
823
833
http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-2950-1&slc_lang=fa&sid=1
Z
Harsini
زهرا
هرسینی
t.harsini@yahoo.com
100319475328460031227
100319475328460031227
No
Department of Pathology, Babol Branch, Islamic Azad University, Babol, Iran
گروه پاتولوژی، واحد بابل، دانشگاه آزاد اسلامی، بابل، ایران
SM
Hosseini
سید محمد
حسینی
dr_hosseini2323@yahoo.com
100319475328460031228
100319475328460031228
Yes
Department of Pathology, Babol Branch, Islamic Azad University, Babol, Iran
گروه پاتولوژی، واحد بابل، دانشگاه آزاد اسلامی، بابل، ایران
F
Pourabdolhossein
فرشته
پورعبدالحسین
ans_dep@yahoo.com
100319475328460031229
100319475328460031229
No
Department of Physiology, Babol University of Medical Sciences, Babol, Iran
گروه فیزیولوژی، دانشگاه علوم پزشکی بابل، بابل، ایران