fa بررسی تغییرات هیستولوژیک و بیان ژن‌های اینترلوکین 8، 12 و اینترفرون بتا در بافت کبدی به دنبال مصرف پروبیوتیک لاکتوباسیلوس رامنوسوس GG در رت‌های مدل استئاتوزیس فیزیولوژی Physiology پژوهشي Research <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">زمینه و هدف</span></span></span></b><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">: مصرف پروبیوتیک قادر به تنظیم هموستاز سیستم گوارشی و کنترل بیماری‌های متابولیکی مرتبط با دستگاه گوارش می باشد. لذا هدف از این مطالعه تعیین و بررسی تغییرات هیستولوژیک و بیان ژن اینترلوکین 12، 8 و اینترفرون بتا بافت کبدی به دنبال مصرف 5 هفته ای پروبیوتیک‌ لاکتوباسیلوس رامنوسوس</span></span></span><b> </b><span dir="LTR" style="font-size:8.0pt"><span arial="" style="font-family:"><span style="color:black">GG</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">در رت‌های مدل استئاتوزیس بود.</span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">روش بررسی</span></span></span></b><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">: در این مطالعه تجربی که در سال 1398 انجام شد، </span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">32 سر موش صحرایی نر ویستار با سن 8 هفته و وزن 180 تا 200 گرم وارد مطالعه شدند و </span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">به طور تصادفی به 4 گروه(هر گروه 8 سر)؛ کنترل(</span></span></span><span dir="LTR" style="font-size:8.0pt"><span arial="" style="font-family:"><span style="color:black">CON</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">)، استئاتوزیس(</span></span></span><span dir="LTR" style="font-size:8.0pt"><span arial="" style="font-family:"><span style="color:black">ST</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">)،</span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">پروبیوتیک(</span></span></span><span dir="LTR" style="font-size:8.0pt"><span arial="" style="font-family:"><span style="color:black">PRO</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">) و استئاتوزیس+پروبیوتیک(</span></span></span><span dir="LTR" style="font-size:8.0pt"><span arial="" style="font-family:"><span style="color:black">ST+PRO</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">) تقسیم شدند.</span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">رت‌ها با تزریق داخل صفاقی 140 میلی‌گرم بر کیلوگرم تتراسایکلین(آنتی‌بیوتیکی که برای ایجاد استئاتوز شناخته شده) مدل شدند. مقدار 10⁷ واحد کلنی تشکیل دهنده(</span></span></span><span dir="LTR" style="font-size:8.0pt"><span arial="" style="font-family:"><span style="color:black">cfu</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">) </span></span></span><span dir="LTR" style="font-size:8.0pt"><span arial="" style="font-family:"><span style="color:black">LGG</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> برای گروه‌های پروبیوتیک به مدت 5 هفته(5 روز در هفته، به صورت گاواژ) در نظر گرفته شد. برای بررسی تغییرات هیستولوژی از رنگ‌آمیزی هماتوکسیلین ائوزین استفاده شد. هم‌چنین تغییرات بیان ژنی </span></span></span><span dir="LTR" style="font-size:8.0pt"><span arial="" style="font-family:"><span style="color:black">IL-12</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">، </span></span></span><span dir="LTR" style="font-size:8.0pt"><span arial="" style="font-family:"><span style="color:black">IL-8</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> و </span></span></span><span dir="LTR" style="font-size:8.0pt"><span arial="" style="font-family:"><span style="color:black">IFN-&beta;</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> از روش </span></span></span><span dir="LTR" style="font-size:8.0pt"><span arial="" style="font-family:"><span style="color:black">RT-PCr</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> استفاده شد. داده‌های جمع‌آوری شده با استفاده از نرم‌افزار آماری آنالیز واریانس یک طرفه تجزیه و تحلیل شدند. </span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">یافته‌ها</span></span></span></b><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">: هیستولوژی بافت کبد پس از مدل </span></span></span><span dir="LTR" style="font-size:8.0pt"><span arial="" style="font-family:"><span style="color:black">ST</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">‌ به دنبال مصرف 5 هفته‌ای پروبیوتیک بهبود پیدا کرد. گروه </span></span></span><span dir="LTR" style="font-size:8.0pt"><span arial="" style="font-family:"><span style="color:black">ST</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> افزایش معنی‌دار در بیان ژن&nbsp; </span></span></span><span dir="LTR" style="font-size:8.0pt"><span arial="" style="font-family:"><span style="color:black">IL-12</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">، </span></span></span><span dir="LTR" style="font-size:8.0pt"><span arial="" style="font-family:"><span style="color:black">IL-8</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> و </span></span></span><span dir="LTR" style="font-size:8.0pt"><span arial="" style="font-family:"><span style="color:black">IFN-&beta;</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> را نشان داد(05/0</span></span></span><span dir="LTR" style="font-size:8.0pt"><span arial="" style="font-family:"><span style="color:black">p<</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">). در مقایسه با گروه </span></span></span><span dir="LTR" style="font-size:8.0pt"><span arial="" style="font-family:"><span style="color:black">ST</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> نیز گروه‌های </span></span></span><span dir="LTR" style="font-size:8.0pt"><span arial="" style="font-family:"><span style="color:black">PRO</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">‌ و </span></span></span><span dir="LTR" style="font-size:8.0pt"><span arial="" style="font-family:"><span style="color:black">ST+PRO</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> کاهش معنی‌دار در بیان ژن&nbsp; </span></span></span><span dir="LTR" style="font-size:8.0pt"><span arial="" style="font-family:"><span style="color:black">IL-12</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">، </span></span></span><span dir="LTR" style="font-size:8.0pt"><span arial="" style="font-family:"><span style="color:black">IL-8</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> و </span></span></span><span dir="LTR" style="font-size:8.0pt"><span arial="" style="font-family:"><span style="color:black">IFN-&beta;</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"> را نشان دادند(05/0</span></span></span><span dir="LTR" style="font-size:8.0pt"><span arial="" style="font-family:"><span style="color:black">p<</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">).</span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">نتیجه‌گیری</span></span></span></b><span lang="FA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black">: به نظر می‌رسد مصرف پروبیوتیک با تنظیم منفی برخی ریسک فاکتورهای سلولی قادر به بهبود سلولی و بافتی کبدی در افراد با مشکلات کبد چرب باشد. با این وجود نیاز به پژوهش‌های بیشتر به ویژه در نمونه انسانی است. </span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:Yagut"><span style="color:black"></span></span></span></b></span></span></span></span></span><br> &nbsp; <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:10.0pt"><span arial="" style="font-family:"><span style="color:black">Background & aim: </span></span></span></b><span style="font-size:10.0pt"><span arial="" style="font-family:"><span style="color:black">Probiotic consumption can regulate the homeostasis of the digestive system and control metabolic diseases related to the digestive system. Therefore, the purpose of the present study was to determine and investigate histological changes and gene expression of interleukin 12, 8 and interferon beta in liver tissue after 5 weeks of consumption of <i>Lactobacillus rhamnosus </i>GG probiotic in steatosis model rats.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:10.0pt"><span arial="" style="font-family:"><span style="color:black"></span></span></span></b></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:10.0pt"><span arial="" style="font-family:"><span style="color:black">Methods</span></span></span></b><span style="font-size:10.0pt"><span arial="" style="font-family:"><span style="color:black">: In the present experimental study conducted in 2018, 32 male Wistar rats aged 8 weeks and weighing 180 to 200 grams were included in the study and randomly divided into 4 groups (8 in each group); Control(CON), steatosis(ST), probiotic(PRO) and steatosis + probiotic(ST+PRO) were divided. Rats were modeled by intraperitoneal injection of 140 mg/kg of tetracycline (an antibiotic known to cause steatosis). The value of 107 colony forming units(cfu) of LGG was considered for probiotic groups for 5 weeks (5 days per week, by gavage). Hematoxylin and eosin staining was used to examine histological changes. Also, IL-12, IL-8 and IFN-&beta; gene expression changes were used by RT-PCR method. The collected data were analyzed using one-way analysis of variance statistical software.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:10.0pt"><span arial="" style="font-family:"><span style="color:black"></span></span></span></b></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:10.0pt"><span arial="" style="font-family:"><span style="color:black">Results</span></span></span></b><span style="font-size:10.0pt"><span arial="" style="font-family:"><span style="color:black">:</span></span></span><span style="font-size:10.0pt"><span arial="" style="font-family:"><span style="color:black"> Liver tissue histology improved after 5 weeks of probiotic consumption after ST model. ST group showed a significant increase in IL-12, IL-8 and IFN-&beta; gene expression (p<0.05). Compared to the ST group, the PRO and ST+PRO groups showed a significant decrease in IL-12, IL-8 and IFN-&beta; gene expression (p<0.05).</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:10.0pt"><span arial="" style="font-family:"><span style="color:black"></span></span></span></b></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:10.0pt"><span arial="" style="font-family:"><span style="color:black">Conclusion</span></span></span></b><span style="font-size:10.0pt"><span arial="" style="font-family:"><span style="color:black">: It appeared that the consumption of probiotics is able to improve the cellular and tissue of the liver in people with fatty liver problems by negatively regulating some cellular risk factors. However, further studies are required, especially in human samples.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:10.0pt"><span arial="" style="font-family:"><span style="color:black"></span></span></span></b></span></span></span><br> <br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:9.0pt"><span arial="" style="font-family:"><span style="color:black"></span></span></span></b></span></span></span> استئاتوزیس کبدی, پروبیوتیک, اینترلوکین, اینترفرون بتا  Hepatic Steatosis, Probiotic, Interleukin, Beta Interferon 551 563 http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-2667-1&slc_lang=fa&sid=1 F Rostamkhani فاطمه رستم خانی Shirinrostamkhani@yahoo.com 100319475328460026080 100319475328460026080 Yes Department of Biology, Yadegar-e-Imam Khomeini(RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran گروه زیست شناسی، واحد یادگار امام خمینی (ره) شهر ری، دانشگاه آزاد اسلامی، تهران، ایران