Armaghane Danesh
ارمغان دانش
armaghanj
Medical Sciences
http://armaghanj.yums.ac.ir
1
admin
1728-6506
1728-6514
10.61882/armaghanj
en
jalali
1401
4
1
gregorian
2022
7
1
27
4
online
1
fulltext
fa
تعیین گونههای مولد لیشمانیوز جلدی در استان کهگیلویه و بویراحمد با روش مولکولی: یک مطالعه گذشته نگر
Identification of Cutaneous Leishmaniasis-causing Species Using Semi-Nested PCR in Kohgiluyeh and Boyer-Ahmad Province During 2013-2019
انگل شناسی
Parasitology
پژوهشي
Research
<span style="background: white;"><span style="line-height: normal;"><span style="unicode-bidi: embed;"><span style="direction: rtl;"><span style="-ms-text-justify: inter-ideograph;"><span lang="FA" style="font-size: 10pt;"><span style="color: black;"><span style="font-family: Yagut;">زمینه و هدف: </span></span></span><span lang="FA" style="font-size: 10pt;"><span style="color: black;"><span style="font-family: Yagut;">لیشمانیوز جلدی یا سالک، در ایران به وسیله دو گونه از انگل لیشمانیا شامل </span></span></span><i><span dir="LTR" style="font-size: 8pt;"><span style="color: black;"><span style="font-family: "Arial","sans-serif";">L.major</span></span></span></i><span lang="FA" style="font-size: 10pt;"><span style="color: black;"><span style="font-family: Yagut;"> و </span></span></span><i><span dir="LTR" style="font-size: 8pt;"><span style="color: black;"><span style="font-family: "Arial","sans-serif";">L.tropica</span></span></span></i><span style="font-size: 10pt;"><span style="color: black;"><span style="font-family: Yagut;"> ایجاد میشود. در طی سالهای اخیر، این بیماری در مناطق مختلف استان کهگیلویه و بویراحمد گزارش شده است. با توجه به تفاوتهای فاحش در نوع مخازن حیوانی و ناقل بیولوژیک، شناسایی عامل ایجاد کننده بیماری میتواند در برنامهریزیهای لازم جهت کنترل بیماری مؤثر باشد. <a><font color="#000000">لذا هدف از این مطالعه تعیین </font></a></span></span></span><span lang="AR-SA" style="font-size: 10pt;"><span style="color: black;"><span style="font-family: Yagut;">گونههای مولد لیشمانیوز جلدی در استان کهگیلویه و بویراحمد با روش مولکولی<a><font color="#000000">: یک مطالعه گذشته </font></a></span></span></span><span lang="AR-SA" style="font-size: 8pt;"><span style="color: black;"><span style="font-family: "Arial","sans-serif";"><a href="#_msocom_1" id="_anchor_1" language="JavaScript" name="_msoanchor_1" on<illegal tag>mouseout="msoCommentHide('_com_1')" on<illegal tag>mouseover="msoCommentShow('_anchor_1','_com_1')"><font color="#0066cc">[p1]</font></a> </span></span></span><span lang="AR-SA" style="font-size: 10pt;"><span style="color: black;"><span style="font-family: Yagut;">نگر بود.</span></span></span><span lang="AR-SA" style="font-size: 8pt;"><span style="color: black;"><span style="font-family: "Arial","sans-serif";"><a href="#_msocom_2" id="_anchor_2" language="JavaScript" name="_msoanchor_2" on<illegal tag>mouseout="msoCommentHide('_com_2')" on<illegal tag>mouseover="msoCommentShow('_anchor_2','_com_2')"><font color="#0066cc">[p2]</font></a> </span></span></span></span></span></span></span></span><br>
<span style="background: white;"><span style="line-height: normal;"><span style="unicode-bidi: embed;"><span style="direction: rtl;"><span style="-ms-text-justify: inter-ideograph;"> </span></span></span></span></span><br>
<span style="background: white;"><span style="unicode-bidi: embed;"><span style="direction: rtl;"><span style="-ms-text-justify: inter-ideograph;"><span style="tab-stops: 56.25pt;"><b><span lang="FA" style="font-size: 10pt;"><span style="color: black;"><span style="line-height: 115%;"><span style="font-family: Yagut;">روش بررسی:</span></span></span></span></b><span lang="FA" style="font-size: 10pt;"><span style="color: black;"><span style="line-height: 115%;"><span style="font-family: Yagut;"> در این مطالعه توصیفی مقطعی و گذشته نگر برای تعیین گونه لیشمانیا، تعداد 52 نمونه لام رنگآمیزی شده با رنگ گیمسا از آزمایشگاههای مختلف در سطح شهرهای استان، بین سالهای 1393 تا 1397 جمعآوری و با استفاده از میکروسکوپ نوری از نظر وجود جسم لیشمن بررسی شدند. پس از استخراج </span></span></span></span><i><span dir="LTR" style="font-size: 8pt;"><span style="color: black;"><span style="line-height: 115%;"><span style="font-family: "Arial","sans-serif";">DNA</span></span></span></span></i><span style="font-size: 10pt;"><span style="color: black;"><span style="line-height: 115%;"><span style="font-family: Yagut;"> موجود در سطح لامها، با استفاده از پرایمرهای اختصاصی </span></span></span></span><i><span dir="LTR" style="font-size: 8pt;"><span style="color: black;"><span style="line-height: 115%;"><span style="font-family: "Arial","sans-serif";">LINR4</span></span></span></span></i><span lang="FA" style="font-size: 10pt;"><span style="color: black;"><span style="line-height: 115%;"><span style="font-family: Yagut;"> و </span></span></span></span><i><span dir="LTR" style="font-size: 8pt;"><span style="color: black;"><span style="line-height: 115%;"><span style="font-family: "Arial","sans-serif";">LIN17</span></span></span></span></i> <span lang="FA" style="font-size: 10pt;"><span style="color: black;"><span style="line-height: 115%;"><span style="font-family: Yagut;">با روش </span></span></span></span><span dir="LTR" style="font-size: 8pt;"><span style="color: black;"><span style="line-height: 115%;"><span style="font-family: "Arial","sans-serif";">PCR</span></span></span></span><span lang="FA" style="font-size: 10pt;"><span style="color: black;"><span style="line-height: 115%;"><span style="font-family: Yagut;"> گونه انگل لیشمانیا شناسایی گردید. دادهها با استفاده از آزمون آماری کای اسکور تجزیه و تحلیل شدند.</span></span></span></span></span></span></span></span></span><br>
<span style="background: white;"><span style="unicode-bidi: embed;"><span style="direction: rtl;"><span style="-ms-text-justify: inter-ideograph;"><b><span lang="FA" style="font-size: 10pt;"><span style="color: black;"><span style="line-height: 115%;"><span style="font-family: Yagut;">یافتهها:</span></span></span></span></b><span lang="FA" style="font-size: 10pt;"><span style="color: black;"><span style="line-height: 115%;"><span style="font-family: Yagut;"> از کل 52 نمونه با زخمهای لیشمانیوز جلدی، 26 نمونه(50 درصد) مربوط به افراد مذکر و 26 نمونه(50درصد) مربوط به افراد مؤنث بودند. میانگین سن افراد در مطالعه 32/23 سال( با انحرافمعیار42/18</span></span></span></span><span lang="FA" style="font-size: 10pt;"><span style="color: black;"><span style="line-height: 115%;"><span style="font-family: "Times New Roman","serif";">±</span></span></span></span><span lang="FA" style="font-size: 10pt;"><span style="color: black;"><span style="line-height: 115%;"><span style="font-family: Yagut;">) بود. از کل 52 نمونه، آماستیگوت انگل(جسم </span></span></span></span><i><span dir="LTR" style="font-size: 8pt;"><span style="color: black;"><span style="line-height: 115%;"><span style="font-family: "Arial","sans-serif";">Leishman</span></span></span></span></i><span lang="FA" style="font-size: 10pt;"><span style="color: black;"><span style="line-height: 115%;"><span style="font-family: Yagut;">)</span></span></span></span> <span lang="FA" style="font-size: 10pt;"><span style="color: black;"><span style="line-height: 115%;"><span style="font-family: Yagut;">در 36 مورد(2/69 درصد) آنها با مشاهده مستقیم به کمک میکروسکوپ نوری رویت شد و 43 نمونه(7/82 درصد) در </span></span></span></span><i><span dir="LTR" style="font-size: 8pt;"><span style="color: black;"><span style="line-height: 115%;"><span style="font-family: "Arial","sans-serif";">PCR</span></span></span></span></i><span lang="FA" style="font-size: 10pt;"><span style="color: black;"><span style="line-height: 115%;"><span style="font-family: Yagut;"> مثبت شد. گونه آلوده کننده در تمامی افراد ساکن دهدشت و یاسوج </span></span></span></span><i><span dir="LTR" style="font-size: 8pt;"><span style="color: black;"><span style="line-height: 115%;"><span style="font-family: "Arial","sans-serif";">L.major</span></span></span></span></i><span lang="FA" style="font-size: 10pt;"><span style="color: black;"><span style="line-height: 115%;"><span style="font-family: Yagut;"> و در گچساران </span></span></span></span><i><span dir="LTR" style="font-size: 8pt;"><span style="color: black;"><span style="line-height: 115%;"><span style="font-family: "Arial","sans-serif";">L.tropica</span></span></span></span></i><span lang="FA" style="font-size: 10pt;"><span style="color: black;"><span style="line-height: 115%;"><span style="font-family: Yagut;"> بود.</span></span></span></span></span></span></span></span><br>
<span style="background: white;"><span style="unicode-bidi: embed;"><span style="direction: rtl;"><span style="-ms-text-justify: inter-ideograph;"><b><span lang="FA" style="font-size: 10pt;"><span style="color: black;"><span style="line-height: 115%;"><span style="font-family: Yagut;">نتیجهگیری:</span></span></span></span></b> <span lang="FA" style="font-size: 10pt;"><span style="color: black;"><span style="line-height: 115%;"><span style="font-family: Yagut;">نتایج به دست آمده از این مطالعه نشان داد که گونهی </span></span></span></span><i><span dir="LTR" style="font-size: 8pt;"><span style="color: black;"><span style="line-height: 115%;"><span style="font-family: "Arial","sans-serif";">L.major</span></span></span></span></i><span style="font-size: 10pt;"><span style="color: black;"><span style="line-height: 115%;"><span style="font-family: Yagut;"> عامل لیشمانیوز جلدی در دهدشت و یاسوج و گونهی </span></span></span></span><i><span dir="LTR" style="font-size: 8pt;"><span style="color: black;"><span style="line-height: 115%;"><span style="font-family: "Arial","sans-serif";">L.tropica</span></span></span></span></i><span style="font-size: 10pt;"><span style="color: black;"><span style="line-height: 115%;"><span style="font-family: Yagut;"> در گچساران میباشند. بنابر این مبارزه با مخازن حیوانی و قطع زنجیره انتقال بر اساس نوع انگل به دست آمده در این تحقیق مورد نیاز است. </span></span></span></span></span></span></span></span>
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<div id="_com_1" language="JavaScript" on<illegal tag>mouseout="msoCommentHide('_com_1')" on<illegal tag>mouseover="msoCommentShow('_anchor_1','_com_1')"><span dir="RTL" lang="AR-SA" style="font-family: "Arial","sans-serif";"><font color="#000000"><font size="2"> </font></font></span></div>
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<span style="background: white;"><span style="line-height: normal;"><span style="-ms-text-justify: inter-ideograph;"><b><span style="font-size: 10pt;"><span style="color: black;"><span style="font-family: "Arial","sans-serif";">Background & aim: </span></span></span></b><span lang="EN" style="font-size: 10pt;"><span style="color: black;"><span style="font-family: "Arial","sans-serif";">Cutaneous leishmaniasis or leishmaniasis in Iran is caused by two species of Leishmania parasites including: <i>L. major</i> and <i>L. tropica</i>.</span></span></span> <span lang="EN" style="font-size: 10pt;"><span style="color: black;"><span style="font-family: "Arial","sans-serif";">In recent years, the disease has been reported in different parts of Kohgiluyeh and Boyer-Ahmad Province. Due to the huge differences in the type of animal reservoirs and biological carriers, identifying the causative agent can be effective in the necessary planning to control the disease.</span></span></span> <span lang="EN" style="font-size: 10pt;"><span style="color: black;"><span style="font-family: "Arial","sans-serif";">Therefore, the aim of the present study was to determine the causative species of cutaneous leishmaniasis in Kohgiluyeh and Boyer Ahmad province, Iran, by molecular methods: a retrospective study.</span></span></span></span></span></span><br>
<span style="background: white;"><span style="line-height: normal;"><span style="-ms-text-justify: inter-ideograph;"><b> </b></span></span></span><br>
<span style="background: white;"><span style="line-height: normal;"><span style="-ms-text-justify: inter-ideograph;"><b><span lang="EN" style="font-size: 10pt;"><span style="color: black;"><span style="font-family: "Arial","sans-serif";">Methods</span></span></span></b><span lang="EN" style="font-size: 10pt;"><span style="color: black;"><span style="font-family: "Arial","sans-serif";">: The present cross-sectional and retrospective descriptive study was conducted to determine the species of Leishmania, 52 samples of smears stained with Giemsa dye were collected from different laboratories in the cities of the province between 2013 and 2017 and were examined using light microscope for the presence of Leishman's body. After extracting the DNA present on the surface of slides, using specific primers LINR4 and LIN17, the species of Leishmania parasite was identified by PCR method. The data were analyzed using chi-score statistical test.</span></span></span></span></span></span><br>
<span style="background: white;"><span style="line-height: normal;"><span style="-ms-text-justify: inter-ideograph;"><b> </b></span></span></span><br>
<span style="background: white;"><span style="line-height: normal;"><span style="-ms-text-justify: inter-ideograph;"><b><span style="font-size: 10pt;"><span style="color: black;"><span style="font-family: "Arial","sans-serif";">Results</span></span></span></b><span style="font-size: 10pt;"><span style="color: black;"><span style="font-family: "Arial","sans-serif";">:</span></span></span><span lang="EN" style="font-size: 10pt;"><span style="color: black;"><span style="font-family: "Arial","sans-serif";"> Out of 52 samples with cutaneous leishmaniasis lesions, 26 (50%) were male and 26 (50%) were female.</span></span></span> <span lang="EN" style="font-size: 10pt;"><span style="color: black;"><span style="font-family: "Arial","sans-serif";">The mean age of the participants in the study was 23.32 years (with a standard deviation of ±18.42). Out of 52 samples, parasitic amastigotes (Leishman's body) were observed in 36 cases (69.2%) by direct observation using a light microscope and 43 samples (82.7%) were positive for PCR.</span></span></span> <span lang="EN" style="font-size: 10pt;"><span style="color: black;"><span style="font-family: "Arial","sans-serif";">Infectious species in all residents of Dehdasht and Yasuj were <i>L. major</i> and <i>L. tropica</i> in Gachsaran.</span></span></span></span></span></span><br>
<span style="background: white;"><span style="line-height: normal;"><span style="-ms-text-justify: inter-ideograph;"><b> </b></span></span></span><br>
<span style="background: white;"><span style="line-height: normal;"><span style="-ms-text-justify: inter-ideograph;"><b><span lang="EN" style="font-size: 10pt;"><span style="color: black;"><span style="font-family: "Arial","sans-serif";">Conclusion</span></span></span></b><span lang="EN" style="font-size: 10pt;"><span style="color: black;"><span style="font-family: "Arial","sans-serif";">: the results of the present study indicated that <i>L.major</i> and <i>L.tropica</i> species were the cause of cutaneous leishmaniasis in Dehdasht, Yasuj, and Gachsaran.</span></span></span> <span lang="EN" style="font-size: 10pt;"><span style="color: black;"><span style="font-family: "Arial","sans-serif";">Therefore, further studies are suggested to identify vectors and reservoirs of cutaneous leishmaniasis parasites in Kohgiluyeh and Boyer-Ahmad province, Iran.</span></span></span></span></span></span><br>
<span style="background: white;"><span style="line-height: normal;"><span style="-ms-text-justify: inter-ideograph;"><b> </b></span></span></span><br>
<span style="background: white;"><span style="line-height: normal;"><span style="-ms-text-justify: inter-ideograph;"><span lang="EN" style="font-size: 10pt;"><span style="color: black;"><span style="font-family: "Arial","sans-serif";"> </span></span></span></span></span></span><br>
<span style="background: white;"><span style="line-height: normal;"><span style="-ms-text-justify: inter-ideograph;"><b> </b></span></span></span>
لیشمانیوز جلدی, گونه, لیشمانیا تروپیکا, لیشمانیا ماژور
Cutaneous leishmaniasis, Species, L.tropica, L.major, Iran.
497
506
http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-1-576&slc_lang=fa&sid=1
N
Arefkhah
نصیر
عارف خواه
nassir162@gmail.com
100319475328460025769
100319475328460025769
No
Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
مرکز تحقیقات سلولی و ملکولی دانشگاه علوم پزشکی یاسوج، یاسوج، ایران
S
Shafiei
ساسان
شفیعی
sasan.shafee@yahoo.com
100319475328460025770
100319475328460025770
No
Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
مرکز تحقیقات سلولی و ملکولی دانشگاه علوم پزشکی یاسوج، یاسوج، ایران
A
Saadat nia
امین اله
سعادت نیا
100319475328460025771
100319475328460025771
No
Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
مرکز تحقیقات سلولی و ملکولی دانشگاه علوم پزشکی یاسوج، یاسوج، ایران
A
Moshfe
عبدالعلی
مشفع
amoshfea@yahoo.com
2431776832
100319475328460025768
Yes
Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
مرکز تحقیقات سلولی و ملکولی دانشگاه علوم پزشکی یاسوج، یاسوج، ایران