Armaghane Danesh
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armaghanj
Medical Sciences
http://armaghanj.yums.ac.ir
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admin
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1728-6514
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gregorian
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تأثیر شدت تمرین هوازی تناوبی بر بیان ژن Bcl-2 و Bax در موش های صحرایی مبتلا به آنفارکتوس میوکارد
Effect of Frequency Aerobic Exercise on Expression of Bcl-2 and Bax Gene in Mice With Myocardial Infarction
فیزیولوژی ورزش
Sport Physiology
پژوهشي
Research
<strong><span style="font-size: 12pt;"><span style="font-family: yagut;"><span style="color: black;">چکیده</span></span></span></strong><br>
<strong><span style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">زمینه و هدف:</span></span></span></span></strong><span style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> آنفارکتوس میوکارد</span></span></span></span><span style="top: -0.5pt; position: relative;"><span style="font-size: 7pt;"><span style="font-family: yagut;"><span style="color: black;">(</span></span></span></span><span dir="LTR" style="top: -0.5pt; position: relative;"><span style="font-size: 7pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">MI</span></span></span></span><span style="top: -0.5pt; position: relative;"><span style="font-size: 7pt;"><span style="font-family: yagut;"><span style="color: black;">)</span></span></span></span><span style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">، انهدام و مرگ سلولی دایم و غیرقابل برگشت بخشی از میوکارد است. تمرینهای بدنی باعث </span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">کاهش آپوپتوز عضله</span></span></span> <span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">قلبی شده، ولی فرایند مولکولی</span></span></span> <span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">آن</span></span></span> <span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">در</span></span></span> <span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">پاسخ</span></span></span> <span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">به</span></span></span> <span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">فعالیت</span></span></span> <span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">و همچنین شدت مناسب فعالیت هنوز</span></span></span> <span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">معلوم</span></span></span> <span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">نیست.</span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> احتمال میرود فعالیت ورزشی، با تأثیر بر مهم ترین عوامل مؤثر بر فرآیند آپوپتوزیس بتواند باعث جلوگیری از مرگ سلولی گردد. </span></span></span> <span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">بر این اساس هدف مطالعه حاضر بررسی تأثیر شدت تمرین تناوبی هوازی بر روند آپوپتوز در موشهای صحرایی مبتلا به آنفارکتوس میوکارد بود.</span></span></span><br>
<br>
<strong><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">روش </span></span></span></strong><strong><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">بررسی</span></span></span></strong><strong><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">:</span></span></span></strong><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> در این مطالعه تجربی 18 سر </span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">موش صحرایی </span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">نر نژاد ویستار 10 هفتهای مبتلا به آنفارکتوس میوکارد در سه گروه </span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">تمرین تناوبی با شدت بالا</span></span></span><span style="font-size: 7pt;"><span style="font-family: yagut;"><span style="color: black;">(</span></span></span><span dir="LTR"><span style="font-size: 7pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">HIIT</span></span></span></span><span style="font-size: 7pt;"><span style="font-family: yagut;"><span style="color: black;">)</span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">،</span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> شدت پایین</span></span></span><span style="font-size: 7pt;"><span style="font-family: yagut;"><span style="color: black;">(</span></span></span><span dir="LTR"><span style="font-size: 7pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">LIIT</span></span></span></span><span style="font-size: 7pt;"><span style="font-family: yagut;"><span style="color: black;">)</span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> و گروه کنترل(بدون تمرین)</span></span></span> <span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">قرار گرفتند.</span></span></span> <span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">غلظت</span></span></span><span dir="LTR"><span style="font-size: 8pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">Bcl-</span></span></span></span><span dir="LTR"><span style="font-size: 8pt;"><span style="font-family: tahoma,sans-serif;"><span style="color: black;">2</span></span></span></span> <span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">و</span></span></span> <span dir="LTR"><span style="font-size: 8pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">Bax</span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> کاردیومیوسیتی به عنوان نشانگران اصلی آپوپتوز با روش </span></span></span><span dir="LTR"><span style="font-size: 8pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">qRT-PCR</span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> سنجیده شد.</span></span></span><br>
<br>
<span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> <strong>یافته ها:</strong> مقادیر</span></span></span><span dir="LTR"><span style="font-size: 8pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">Bcl-2</span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> در</span></span></span> <span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">گروه</span></span></span> <span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">تمرین تناوبی با شدت بالا</span></span></span><span style="font-size: 8pt;"><span style="font-family: yagut;"><span style="color: black;">(</span></span></span><span dir="LTR"><span style="font-size: 8pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">HIIT</span></span></span></span><span style="font-size: 8pt;"><span style="font-family: yagut;"><span style="color: black;">)</span></span></span> <span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">به طور غیر معنیداری بیشتر از گروه کنترل</span></span></span><span style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">(077/0</span></span></span></span><span dir="LTR" style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">p</span></span></span></span><span dir="LTR" style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="color: black;"><span style="font-family: calibri;">=</span></span></span></span><span style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">)،</span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> در گروه</span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> تمرین تناوبی با شدت پایین</span></span></span><span style="font-size: 8pt;"><span style="font-family: yagut;"><span style="color: black;">(</span></span></span><span dir="LTR"><span style="font-size: 8pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">LIIT</span></span></span></span><span style="font-size: 8pt;"><span style="font-family: yagut;"><span style="color: black;">)</span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">(</span></span></span><span style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">017/0</span></span></span></span><span dir="LTR" style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">p</span></span></span></span><span dir="LTR" style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="color: black;"><span style="font-family: calibri;">=</span></span></span></span><span style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">)</span></span></span></span> <span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">به طور معنیداری بیشتر از گروه کنترل بود. مقادیر</span></span></span> <span dir="LTR"><span style="font-size: 8pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">Bax</span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> نیز در گروه </span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">تمرین تناوبی با شدت بالا</span></span></span> <span style="font-size: 8pt;"><span style="font-family: yagut;"><span style="color: black;">(</span></span></span><span dir="LTR"><span style="font-size: 8pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">HIIT</span></span></span></span><span style="font-size: 8pt;"><span style="font-family: yagut;"><span style="color: black;">)</span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">(</span></span></span><span style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">019/0</span></span></span></span><span dir="LTR" style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="font-family: tahoma,sans-serif;"><span style="color: black;">p</span></span></span></span><span dir="LTR" style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="color: black;"><span style="font-family: calibri;">=</span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">)</span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> به طور معنیداری بیشتر از گروه کنترل بود. </span></span></span><span style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">در حالی که در گروه </span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">تمرین تناوبی با شدت پایین</span></span></span><span style="font-size: 8pt;"><span style="font-family: yagut;"><span style="color: black;">(</span></span></span><span dir="LTR"><span style="font-size: 8pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">LIIT</span></span></span></span><span style="font-size: 8pt;"><span style="font-family: yagut;"><span style="color: black;">)</span></span></span> <span style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">افزایش غیر معنیدار نسبت به گروه کنترل داشت(98/0</span></span></span></span><span dir="LTR" style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">p</span></span></span></span><span dir="LTR" style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="color: black;"><span style="font-family: calibri;">=</span></span></span></span><span style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">). مقادیر</span></span></span></span> <span dir="LTR" style="top: -0.5pt; position: relative;"><span style="font-size: 8pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">BCL-2</span></span></span></span><span style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> در گروه </span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">تمرین تناوبی با شدت پایین</span></span></span><span style="font-size: 8pt;"><span style="font-family: yagut;"><span style="color: black;">(</span></span></span><span dir="LTR"><span style="font-size: 8pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">LIIT</span></span></span></span><span style="font-size: 8pt;"><span style="font-family: yagut;"><span style="color: black;">)</span></span></span> <span style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">بیشتر از گروه </span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">تمرین تناوبی با شدت بالا</span></span></span><span style="font-size: 8pt;"><span style="font-family: yagut;"><span style="color: black;">(</span></span></span><span dir="LTR"><span style="font-size: 8pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">HIIT</span></span></span></span><span style="font-size: 8pt;"><span style="font-family: yagut;"><span style="color: black;">)</span></span></span> <span style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">بود(023/0</span></span></span></span><span dir="LTR" style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">p</span></span></span></span><span dir="LTR" style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="color: black;"><span style="font-family: calibri;">=</span></span></span></span><span style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">). مقادیر شاخص</span></span></span></span><span dir="LTR" style="top: -0.5pt; position: relative;"><span style="font-size: 8pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">Bax</span></span></span></span><span style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> در گروه </span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">تمرین تناوبی با شدت بالا</span></span></span><span style="font-size: 8pt;"><span style="font-family: yagut;"><span style="color: black;">(</span></span></span><span dir="LTR"><span style="font-size: 8pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">HIIT</span></span></span></span><span style="font-size: 8pt;"><span style="font-family: yagut;"><span style="color: black;">)</span></span></span> <span style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">بیشتر از گروه</span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> تمرین تناوبی با شدت پایین</span></span></span> <span dir="LTR" style="top: -0.5pt; position: relative;"><span style="font-size: 8pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">LIIT</span></span></span></span><span style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> بود(019/0</span></span></span></span><span dir="LTR" style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">p</span></span></span></span><span dir="LTR" style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="color: black;"><span style="font-family: calibri;">=</span></span></span></span><span style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">). </span></span></span></span><br>
<br>
<span style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> <strong>نتیجه گیری:</strong> تمرین تناوبی </span></span></span></span><span style="top: -0.5pt; position: relative;"><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">از طریق</span></span></span></span> <span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">بیان ژن</span></span></span> <span dir="LTR"><span style="font-size: 8pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">Bcl-2</span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> و</span></span></span> <span dir="LTR"><span style="font-size: 8pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">Bax</span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> و در نتیجه کاهش آپوپتوز درکاردیومیوسیت <span style="top: -0.5pt; position: relative;">پس از وقوع آنفارکتوس میوکارد شده که میزان آن وابسته به شدت تمرین میباشد. به علاوه تمرین تناوبی با شدت پایین اثر بیشتری نسبت به تمرین تناوبی با شدت بالا دارد. براین اساس توصیه میشود در بازتوانی قلبی به شدت تمرین توجه شود.</span></span></span></span><br>
<br>
Abstract<br>
<br>
<strong>Background and Aim:</strong> Myocardial infarction (MI) is a permanent and irreversible cell death and death of the myocardium. Physical training reduces cardiac muscle apoptosis, but its molecular process is still unknown in response to activity and also the intensity of exercise. Exercise activity can be expected to prevent cell death by influencing the most important factors affecting the apoptosis process. Therefore, the aim of this study was to investigate the effect of aerobic exercise intensity on apoptosis in rats with myocardial infarction.<br>
<br>
<strong>Methods</strong>: In this experimental study, 18 male Wistar rats with myocardial infarction were divided into three groups: High intensity periodic exercise (HIIT), Low intensity (LIIT) and Control group (without exercise). Bcl-2 and Bax cardiomyocyte concentrations were evaluated as the main markers of apoptosis by qRT-PCR method.<br>
<br>
<strong>Results</strong>: Bcl-2 values were significantly higher in the high intensity exercise group (HIIT) than in the control group (p = 0.077), in the low intensity periodic exercise group (LIIT) (p = 0.017) were more than the control group. Bax values were significantly higher in the high intensity periodic exercise group (HIIT) (p = 0.019) than in the control group. While in the low intensity exercise group (LIIT), there was no significant increase compared to the control group (p = 0.98). BCL-2 values in the low intensity periodic exercise group (LIIT) were higher than the high intensity periodic exercise group (HIIT) (p = 0.023). Bax index values in the high intensity periodic exercise group (HIIT) were higher than the low intensity exercise group (1.91).<br>
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<strong>Conclusion:</strong> Periodic training was performed by expression of Bcl-2 and <em>Bax</em> gene and as a result of reduction of apoptosis in cardiomyocytes after myocardial infarction, which depends on the intensity of exercise. In addition, low intensity periodic exercises have a greater effect than high intensity periodic exercises. On this basis, it is recommended that attention be paid to intensive training in cardiac rehab.<br>
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<br>
آپوپتوز, آنفارکتوس میوکارد, تمرین تناوبی خیلی شدید, تمرین تناوبی با شدت پایین
Apoptosis, Myocardial infarction, Very intense exercise,Bcl-2 , Bax
781
791
http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-1164-1&slc_lang=fa&sid=1
M
Ghahremani
مهران
قهرمانی
mehran.physiology@gmail.com
100319475328460016849
100319475328460016849
No
Department of Physiology, Islamic Azad University, Tehran, Iran,
گروه فیزیولوژی ورزشی، دانشگاه آزاد اسلامیواحد تهران مرکزی، تهران، ایران،
MA
Azarbaijani
محمد علی
آذربایجانی
mehrangilan@gmail.com
100319475328460016850
100319475328460016850
Yes
Department of Physiology, Tehran University of Medical Sciences, Tehran, Iran
گروه فیزیولوژی ، دانشگاه علوم پزشکی ایران، تهران، ایران
M
Piri
مقصود
پیری
mehrangilan@gmail.com
100319475328460016851
100319475328460016851
No
Department of Physiology, Tehran University of Medical Sciences, Tehran, Iran
گروه فیزیولوژی ، دانشگاه علوم پزشکی ایران، تهران، ایران
A
Raoufi
عاطفه
رئوفی
mehran.physiology@gmail.com
100319475328460016852
100319475328460016852
No
Department of Physiology, Tehran University of Medical Sciences, Tehran, Iran
گروه فیزیولوژی ، دانشگاه علوم پزشکی ایران، تهران، ایران