Armaghane Danesh
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اثر ضد تکثیری نانو نقره سنتز شده با استفاده از گیاه آب بشقابی بر رده سلولی سرطان پستان
Antiprolifration Effect of Silver Nanoparticles Syntethised Using Centlla Asiatica on Breast Cancer Cell Line
تخصصي
Special
پژوهشي
Research
<strong><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">چکیده </span></span></span></strong><br>
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<strong><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">زمینه و هدف</span></span></span></strong><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">:</span></span></span> <span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">استفاده از گیاهان جهت سنتز نانوذرات یکی از روش</span></span></span><span dir="LTR"><span style="font-size: 10pt;"><span><span style="color: black;"></span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">های دوست</span></span></span><span dir="LTR"><span style="font-size: 10pt;"><span><span style="color: black;"></span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">دار محیط زیست به شمار می</span></span></span><span dir="LTR"><span style="font-size: 10pt;"><span><span style="color: black;"></span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">رود. هدف از این تحقیق، سنتز سبز نانوذره نقره با استفاده از عصاره </span></span></span><em><span dir="LTR"><span style="font-size: 7pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">Centella asiatica</span></span></span></span></em><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> و بررسی اثرات سایتوکسیک در رده سلولی </span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">سرطان پستان </span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">بود</span></span></span><span dir="LTR"><span style="font-size: 10pt;"><span style="font-family: times,serif;"><span style="color: black;">.</span></span></span></span><br>
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<strong><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">روش بررسی:</span></span></span></strong><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> در این مطالعه تجربی ریخت</span></span></span><span dir="LTR"><span style="font-size: 10pt;"><span><span style="color: black;"></span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">شناسی نانوذرات نقره زیستی با استفاده از آنالیز میکروسکوپ الکترونی گذاره</span></span></span><span style="font-size: 7pt;"><span style="font-family: yagut;"><span style="color: black;">(</span></span></span><span dir="LTR"><span style="font-size: 7pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">TEM</span></span></span></span><span style="font-size: 7pt;"><span style="font-family: yagut;"><span style="color: black;">)</span></span></span> <span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">و میکروسکوپ الکترونی روبشی </span></span></span><span dir="LTR"><span style="font-size: 7pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">(SEM)</span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> مورد ارزیابی قرار گرفت. از آزمون</span></span></span> <span dir="LTR"><span style="font-size: 7pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">MTT</span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> جهت بررسی اثرات ضد تکثیری نانوذرات زیستی در رده سلولی سرطان پستان</span></span></span><span style="font-size: 7pt;"><span style="font-family: yagut;"><span style="color: black;">(</span></span></span><span dir="LTR"><span style="font-size: 7pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">MCF-7</span></span></span></span><span style="font-size: 7pt;"><span style="font-family: yagut;"><span style="color: black;">)</span></span></span> <span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">و سلول نرمال </span></span></span><span dir="LTR"><span style="font-size: 8pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">(HEK-293)</span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> استفاده شد.آزمون اپوپتوز با استفاده از فلوسایتومتری با کیت</span></span></span><span dir="LTR"><span style="font-size: 8pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">Annexin V</span></span></span></span> <span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> انجام شد.</span></span></span> <span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">داده</span></span></span><span dir="LTR"><span style="font-size: 10pt;"><span><span style="color: black;"></span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">ها با استفاده از آزمون</span></span></span><span dir="LTR"><span style="font-size: 10pt;"><span><span style="color: black;"></span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">های آماری آنوا دو طرفه </span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">تجزیه و تحلیل شدند</span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">.</span></span></span><br>
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<strong><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">یافته­ها:</span></span></span></strong> <span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">ذرات کروی با میانگین </span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">19 </span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">نانومتر سنتز شدند. فعالیت ضدتکثیری در سلول</span></span></span><span dir="LTR"><span style="font-size: 10pt;"><span><span style="color: black;"></span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">های</span></span></span> <span dir="LTR"><span style="font-size: 7pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">MCF-7</span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> با افزایش غلظت و زمان نانوذره افزایش یافت.</span></span></span><span dir="LTR"><span style="font-size: 7pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">IC50</span></span></span></span> <span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> نانوذره نقره در زمان 24 ساعت 76/8</span></span></span> <span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">میکروگرم بر میلی</span></span></span><span dir="LTR"><span style="font-size: 10pt;"><span><span style="color: black;"></span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">لیتر در سلول</span></span></span><span dir="LTR"><span style="font-size: 10pt;"><span><span style="color: black;"></span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">های سرطانی بود. افزایش اپوپتوز در سلول</span></span></span><span dir="LTR"><span style="font-size: 10pt;"><span><span style="color: black;"></span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">های تیمار شده با نانوذره زیستی در غلظت </span></span></span><span dir="LTR"><span style="font-size: 8pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">IC50</span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> نسبت به سلول</span></span></span><span dir="LTR"><span style="font-size: 10pt;"><span><span style="color: black;"></span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">های کنترل دیده شد(001/0</span></span></span><span dir="LTR"><span style="font-size: 10pt;"><span style="font-family: times,serif;"><span style="color: black;">p<</span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">)</span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">. </span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">هم</span></span></span><span dir="LTR"><span style="font-size: 10pt;"><span><span style="color: black;"></span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">چنین القا آپوپتوز بیشتری در برابر نکروز در سلول</span></span></span><span dir="LTR"><span style="font-size: 10pt;"><span><span style="color: black;"></span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">های</span></span></span> <span dir="LTR"><span style="font-size: 7pt;"><span style="font-family: arial,sans-serif;"><span style="color: black;">MCF-7</span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> نسبت به کنترل دیده شد(001/0</span></span></span><span dir="LTR"><span style="font-size: 10pt;"><span style="font-family: times,serif;"><span style="color: black;">p<</span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">)</span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">.</span></span></span><br>
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<strong><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> نتیجه­گیری:</span></span></span></strong><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;"> نانوذرات نقره سنتز شده دارای اثر سمیت سلولی و القا آپوپتوز بر روی سلول</span></span></span><span dir="LTR"><span style="font-size: 10pt;"><span><span style="color: black;"></span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">های سرطانی در الگوی وابسته به غلظت و زمان می</span></span></span><span dir="LTR"><span style="font-size: 10pt;"><span><span style="color: black;"></span></span></span></span><span style="font-size: 10pt;"><span style="font-family: yagut;"><span style="color: black;">باشند.</span></span></span><br>
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<strong>Abstract:</strong><br>
<strong>Background and aim</strong>: The use of plants to synthesize nanoparticles is one of the most environmentally friendly methods. The purpose of this study was to synthesize green silver nanoparticles using Centella asiatica extract and study the effects of cytotoxicity in breast cancer cell line.<br>
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<strong>Methods:</strong> In this experimental study, the morphology of silver nanoparticles was evaluated using transient electron microscopy (TEM) and scanning electron microscopy (SEM). MTT was used to evaluate the anti-proliferative effects of biological nanoparticles in breast cancer cell line (MCF-7) and normal cells (HEK-293). Apoptotic assays were performed using flowchitometry with Annexin V kits. Data were analyzed using two-way ANOVA statistical tests.<br>
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<strong>Results:</strong> Spherical particles were synthesized with an average of 19.19 nm. Antimicrobial activity in MCF-7 cells increased with increasing concentrations and time of nanoparticles. IC50 of silver nanoparticle was 24.88 μg / ml in cancerous cells. Increasing apoptosis was observed in cells treated with biological nanoparticles at IC50 concentration compared to control cells (p <0.001). Also induction of apoptosis in MCF-7 cells was more than necrotizing compared to control (p<0.001).<br>
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<strong>Conclusion:</strong> The synthesized silver nanoparticles have the effect of cellular toxicity and induction of apoptosis on cancer cells in a concentration-time pattern.<br>
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گیاه آب بشقابی, نانو ذرات نقره زیستی, سمیت سلولی
Botanical Plants, Biodegradable Nanoparticles, Cytotoxicity
570
583
http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-39-169&slc_lang=fa&sid=1
SH
Edalat fard
شیما
عدالت فرد
shimaedalatfar@yahoo.com
100319475328460014371
100319475328460014371
No
1Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran
1گروه زیستشناسی، واحد پرند، دانشگاه آزاد اسلامی، پرند، ایران،
SH
Edalat fard
فرزانه
تفویضی
farzanehtafvizi54@gmail.com
100319475328460014372
100319475328460014372
Yes
1Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran
1گروه زیستشناسی، واحد پرند، دانشگاه آزاد اسلامی، پرند، ایران،
M
Bikhof Torbati
مریم
بی خوف تربتی
maryam.bikhof@gmail.com
100319475328460014373
100319475328460014373
No
Department of Biology, College of science, Yadegar - e- Imam Khomeini (RAH) shahre-rey Branch, Islamic Azad University, Tehran, Iran
2گروه زیستشناسی، واحد یادگار امام خمینی (ره) شهرری، دانشگاه آزاد اسلامی، تهران، ایران