fa اثر عصاره آبی برگ گیاه حرا (Avicennia marina) بر هورمون‌های محور هیپوفیزـ گناد و تعداد فولیکول‌های تخمدان در موش‌های‌ صحرایی دیابتی عمومى General پژوهشي Research <p dir="RTL" style="margin: 0cm 0cm 0pt; text-align: right; line-height: normal; unicode-bidi: embed; direction: rtl;"><font color="#000000"><b><span lang="AR-SA" new="" style="font-family: Yagut; font-size: 12pt;" times="">چکید</span></b><b><span color="#000000" dir="RTL" font="" lang="FA" new="" p="" span="" style="font-family: Yagut; font-size: 10pt;" times="">زمینه و هدف:</span></b><span .="" lang="FA" new="" span="" style="font-family: Yagut; font-size: 10pt;" times="">های محور هیپوفیزـ گناد و تعداد فولیکول</span><span lang="FA" new="" style="font-family: Yagut; font-size: 10pt; mso-ascii-font-family: Cambria;" times="">&shy;</span><span color="#000000" dir="RTL" font="" lang="FA" new="" p="" span="" style="font-family: Yagut; font-size: 10pt;" times="">روش بررسی:</span><span 100="" 200="" 32="" dir="LTR" lang="FA" new="" span="" style="font-family: Yagut; font-size: 10pt;" times="">د.</span><span lang="AR-SA" new="" style="font-family: Yagut; font-size: 10pt;" times=""> دیابت در گروه&shy; دیابتی تیمار نشده و گروه&shy;های دیابتی تحت تیمار با عصاره حرا، به وسیله یک&shy;بار تزریق داخل صفاقی 120 میلی&shy;گرم بر کیلوگرم آلوکسان القاء شد. عصاره برگ&shy; گیاه حرا یک روز در میان و به&shy;مدت یک ماه</span><span lang="AR-SA" new="" style="font-family: Yagut; font-size: 10pt;" times=""> به&shy;صورت داخل صفاقی به </span><span lang="AR-SA" new="" style="font-family: Yagut; font-size: 10pt;" times="">گروه&shy;های دیابتی تحت تیمار </span><span lang="AR-SA" new="" style="font-family: Yagut; font-size: 10pt;" times="">تزریق شد. محلول سالین</span><span lang="AR-SA" new="" style="font-family: Yagut; font-size: 10pt;" times=""> به حیوانات گروه&shy; کنترل و دیابتی تیمار نشده، تزریق شد. در پایان دوره تیمار</span><span .="" 5="" dir="LTR" lang="FA" new="" span="" style="font-family: Yagut; font-size: 10pt;" times="">ن و</span><span lang="AR-SA" new="" style="font-family: Yagut; font-size: 10pt;" times=""> ائوزین رنگ‌آمیزی شد. سپس تعداد فولیکول</span><span dir="LTR" style="font-family:;">&shy;</span><span lang="AR-SA" new="" style="font-family: Yagut; font-size: 10pt;" times="">های تخمدان به وسیله میکروسکوپ نوری بررسی شد.</span><span lang="FA" new="" span="" style="font-family: Yagut; font-size: 10pt;" times="">نتایج با استفاده از </span><span lang="AR-SA" new="" style="font-family: Yagut; font-size: 10pt;" times="">آزمون آماری </span><span lang="FA" new="" span="" style="font-family: Yagut; font-size: 10pt;" times="">د.</span></font></p> <p dir="RTL" style="margin: 0cm 0cm 0pt; text-align: justify; line-height: normal; unicode-bidi: embed; direction: rtl;"><b><span dir="LTR" new="" style="font-family:;" times=""><font color="#000000">&nbsp;</font></span></b></p> <p dir="RTL" style="margin: 0cm 0cm 0pt; text-align: justify; line-height: normal; unicode-bidi: embed; direction: rtl;"><font color="#000000"><b><span dir="LTR" lang="FA" new="" span="" style="font-family: Yagut; font-size: 10pt;" times="">ی تیمار نشده به&shy;طور معنی&shy;داری</span><span lang="AR-SA" new="" style="letter-spacing: -0.3pt; font-family: Yagut; font-size: 10pt;" times=""> کاهش یافت</span><span lang="AR-SA" new="" style="font-family: Yagut; font-size: 10pt;" times="">(05/0></span><span dir="LTR" new="" style="font-family:;" times="">p</span><span lang="AR-SA" new="" style="font-family: Yagut; font-size: 10pt;" times="">)</span><span lang="AR-SA" new="" style="letter-spacing: -0.3pt; font-family: Yagut; font-size: 10pt;" times="">. هم‌چنین</span><span lang="FA" new="" span="" style="font-family: Yagut; font-size: 10pt;" times=""> بدوی،</span><span lang="AR-SA" new="" style="font-family: Yagut; font-size: 10pt;" times=""> اولیه، ثانویه و گراف</span><span lang="AR-SA" new="" style="font-family: Yagut; font-size: 10pt;" times=""> در مقایسه با گروه کنترل به&shy;طور معنی&shy;داری کاهش</span><span .="" dir="LTR" lang="FA" new="" span="" style="font-family: Yagut; font-size: 10pt;" times="">ی تیمار نشده، </span><span lang="AR-SA" new="" style="letter-spacing: -0.3pt; font-family: Yagut; font-size: 10pt;" times="">تجویز</span><span lang="AR-SA" new="" style="font-family: Yagut; font-size: 10pt;" times=""> </span><span 100="" 200="" dir="LTR" lang="FA" new="" span="" style="font-family: Yagut; font-size: 10pt;" times="">د</span><span lang="AR-SA" new="" style="font-family: Yagut; font-size: 10pt;" times="">(05/0></span><span dir="LTR" new="" style="font-family:;" times="">p</span><span lang="AR-SA" new="" style="font-family: Yagut; font-size: 10pt;" times="">). هم‌چنین تعداد </span><span lang="AR-SA" new="" style="font-family: Yagut; font-size: 10pt;" times="">جسم زرد، فولیکول&shy;های</span><span lang="FA" new="" span="" style="font-family: Yagut; font-size: 10pt;" times="">ی تیمار نشده</span><span lang="FA" new="" style="font-family: Yagut; font-size: 10pt;" times=""> </span><span lang="AR-SA" new="" style="font-family: Yagut; font-size: 10pt;" times="">به&shy;طور معنی&shy;داری افزایش</span><span color="#000000" dir="RTL" font="" lang="FA" new="" p="" span="" style="font-family: Yagut; font-size: 10pt;" times="">نتیجه&shy;گیری:</span></b><span lang="AR-SA" new="" style="font-family: Yagut; font-size: 10pt;" times=""> ن</span><span lang="FA" new="" span="" style="font-family: Yagut; font-size: 10pt;" times="">در موش&shy;های صحرایی دیابتی است</span><span lang="AR-SA" new="" style="font-family: Yagut; font-size: 10pt;" times="">. هم‌چنین دارای اثر محافظتی بر بافت تخمدان</span><span lang="FA" new="" span="" style="font-family: Yagut; font-size: 10pt;" times="">می&shy;باشد؛ </span><span lang="AR-SA" new="" style="font-family: Yagut; font-size: 10pt;" times="">بنابراین</span><span dir="LTR" lang="AR-SA" new="" style="font-family:;" times=""> </span><span lang="AR-SA" new="" style="font-family: Yagut; font-size: 10pt;" times="">استفاده از گیاه حرا می‌تواند در بهبود اختلالات</span><span dir="LTR" lang="AR-SA" new="" style="font-family:;" times=""> </span><span lang="AR-SA" new="" style="font-family: Yagut; font-size: 10pt;" times="">هورمونی و آسیب بافت تخمدان در بیماران دیابتی مؤثر باشد.</span></font></p> <p dir="RTL" style="margin: 0cm 0cm 0pt; text-align: right; line-height: normal; unicode-bidi: embed; direction: rtl;"><b><span dir="LTR" new="" style="font-family:;" times=""><font color="#000000">&nbsp;</font></span></b></p> <p dir="RTL" style="margin: 0cm 0cm 0pt; text-align: right; line-height: normal; unicode-bidi: embed; direction: rtl;"></p> <strong>ABSTRACT</strong><br> <strong>Background & aim:</strong> Reproductive disorders is one of the major issues in diabetic people and various chemical and herbal medicines has been used for its treatment. Due to the antioxidant and hypoglycemic properties of <em>Avicennia marina</em>, the aim of this study was to determine the effects of mangrove leaves aqueous extract on serum levels of hormones of pituitary- gonadal axis and ovarian follicle numbers in diabetic rats.<br> &nbsp;<br> <strong>Methods: </strong>In the present experimental study, 32 female Wistar rats were allocated into equal groups of control, non-treated diabetic and two diabetic groups treated with mangrove leaves aqueous extract at concentrations of 100 and 200 mg/kg.&nbsp; The diabetes in non-treated diabetic and treated diabetic groups with mangrove extract was induced using an intraperitoneal injection of 120 mg/kg alloxan.<br> Aqueous extract of mangrove leaves were injected to treated diabetic groups, very other day for one month. Saline solution was injected to the animals of control and non-treated diabetic groups. At the end of exprement, serum levels of LH, FSH, estrogen, progesterone and prolactin were measured by ELISA. 5 &micro;m sections of ovarian tissue were prepared and stained with Hematoxylin and Eosin. Then, ovarian follicle numbers were examined by light microscope. Data was analyzed using one-way ANOVA and Post Hoc Tukey statistical tests.<br> &nbsp;<br> <strong>Results:</strong> Compared to control group, serum levels of LH, FSH, estrogen, progesterone and prolactin significantly decreased in non-treated diabetic group (p<0.05). Also, number of corpus luteum, primordial, primary, secondary and graph follicle significantly decreased and number of atresia follicle significantly increased compared to the control group (p<0.05). Compared to non-treated diabetic group, administration of mangrove leaves aqueous extract with concentrations of 100 and 200 mg/kg significantly increased serum levels of LH, FSH, estrogen, progesterone and prolactin (p<0.05). Also, number of corpus luteum, primordial, primary, secondary and graph follicle significantly increased and number of atresia follicle significantly decreased compared to non-treated diabetic group (p<0.05).<br> &nbsp;<br> <strong>Conclusions:</strong> The results indicate significant effect of mangrove leaves extract on increases serum levels of gonadotropins, estrogen, progesterone and prolactin in diabetic rats. Also it has protective effect on ovarian tissue. Therefore, consumption of <em>Avicennia marina</em> could be effective in improving hormonal disorders and ovarian tissue damage in patients with diabetes.<br> &nbsp;<br> &nbsp; دیابت, تخمدان, حرا, گنادوتروپین, موش صحرایی Diabetes, Ovary, Avicennia marina, Gonadotropin, Gonadal Steroid Hormones, Rat 1 17 http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-784-1&slc_lang=fa&sid=1 R Rahbarian راهله رهباریان Rahbarian-ra@gmail.com 100319475328460012591 100319475328460012591 No Department of Biology, Payam-e-Noor University, Tehran, Iran گروه زیست‌شناسی، دانشکده علوم، دانشگاه پیام نور، تهران، ایران SD Sadoughi سید دامون صدوقی damoon.sadughi@gmail.com 100319475328460012592 100319475328460012592 Yes Young Researchers and Elite Club, Mashhad Branch, Islamic Azad University, Mashhad, Iran باشگاه پژوهشگران جوان و نخبگان، واحد مشهد، دانشگاه آزاد اسلامی، مشهد، ایران H Esmaeili Sabzevar حوریه اسماعیلی سبزوار damoon.sadoughi@mshdiau.ac.ir 100319475328460012593 100319475328460012593 No Department of Biology, Payam-e-Noor University, Tehran, Iran گروه زیست‌شناسی، دانشکده علوم، دانشگاه پیام نور، تهران، ایران