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Identification and Determination of Drug Resistant of Candida species isolated from Hospital Acquired Infections
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Kambiz Diba1   , AliReza Chavoshin1 , Nima Hoseyni Jazani1 , Parisa Badie2 , Frazaneh Bonyadi 3, Hamid Alizadeh3 , Elham Shahnazi3 |
1- Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran,
2- Alborzi Professor Research Center for Infectious Diseases, Shiraz University of Medical Sciences, Shiraz, Iran,
3- Research Laboratory of Molecular Mycology and Parasitology, Urmia University of Medical Sciences, Urmia, Iran |
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Abstract: (6210 Views) |
| Background & aim: Currently, the use of antifungal azole group and yeasts resistant to these drugs is increasing. The aim of this study was to isolate and identify the yeasts obtained from candidiasis patients and furthermore determining thier antifungal resistance.
Methods: In the present descriptive study, infections samples were collected from 256 patients with suspected nosocomial candidiasis, then direct exam and culture were performed. Yeast colonies were identified using phenotypic methods, polymerase chain reaction method and enzyme digestion. Data were analyzed using Descriptive statistical tests.
Results: Of sixty isolated yeast, thirty-seven cases of Candida albicans (61.6%), seven cases of C. krusei and C. glabrata (11.6%) each, five cases of C. dubliniensis (8.3%) and four cases of C. tropicalis (6.6%) were indicated. The study showed that the sensitivity of C. albicans and C. cruise species to amphotericin B was negligible in disk diffusion and very sensitve in microdilution.
Conclusion: Inspite of the results of antifungal susceptibility test of strains studied did not show high resistance, but screening for drug-resistant Candida isolates in Candida infection by disk diffusion and microdilution methods for new cases of drug resistance is reasonable. |
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| Keywords: Candida, Drug resistance, Disk diffusion, Microdilution |
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Full-Text [PDF 189 kb]
(1697 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2015/02/27 | Accepted: 2015/02/27 | Published: 2015/02/27
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