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Nanoparticles and Ethylene Diamine Tetra Acetic Acid on Growth Inhibition of Standard Strain of Candida albicans
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F Haghighi   , SH Rodbar Mohammadi 1, P Mohammadi , M Eskandari |
| 1- , Sh.mohammadi@modares.ac.ir |
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Abstract: (4488 Views) |
| Introduction & Objective: In recent years, the incidence of opportunistic fungi has shown a marked increase. Infection caused by common pathogenic fungi is a significant health problem in immune compromised hosts. The present study evaluated antifungal activity of Titanum dioxide nanoparticles and Ethylene Diamine Tetra-acetic Acid against Candida albicans as self-cleaning agent by standard micro dilution test.
Materials & Methods: The present study was conducted at the Medical University of Tarbiyat Modares in 2009. TiO2 nanoparticles were obtained through the hydrolysis of TiCl4 (Titanium tetrachloride). Size and type of these nanoparticles were characterized by scanning electron microscopy (SEM) and X-Ray-Diffraction (XRD). Afterwards, the Minimum Inhibitory Concentration (MIC) and Minimal Fungicide Concentration (MFC) test for TiO2 and EDTA were performed.
Results: Concentration of synthesised TiO2 was 7.03 mg/ml and 5.63 5.63 ×1020 particles/ml. Evaluation of morphology and diameter of the TiO2 nanoparticles with SEM showed that nanoparticles were spherical with diameter between 40-65 nm. MIC50 of 2.2, 1.24 and 0.125 µg/ml respectively. MIC90 and MFC of TiO2, EDTA and fluconazole were 3.51, 2.48 , 0.5 µg/ml and 4.06, 3.1 ,1 µg/ml respectively.
Conclusion: In the present study, using of synthesized TiO2 nanoparticles with chemical method showed a suitable activity against Candida in comparison with Fluconazole. Thus it might represent a good candidates in elimination of Candida in medical from medical devices.
Key Words: |
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| Keywords: Antifungal effect, Candida albicans, nanoparticles. |
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Full-Text [PDF 147 kb]
(1353 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2015/05/9 | Accepted: 2015/05/9 | Published: 2015/05/9
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