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The Effects of Two Species of Daphne, Betulin and Betulinic Acid on Alkaline Phosphatase Activity in Two Human Cancer Cell lines, K562 and MCF-7
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E Panahi Kokhdan   , M Mianabadi 1, H Sadeghi , M Khalaf |
| 1- , m.mianabadi@gu.ac.ir |
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Abstract: (4660 Views) |
| Abstract
Background & aim: Changes of alkaline phosphatase activity is one of the symptoms of many diseases. The aim of this study was to evaluate the effect of two types of Daphne, Betulin and Betulinic acid, on alkaline phosphatase activity in K562 and MCF-7 cell lines, respectively.
Methods: In this study, 106 cancer cell lines of K562 and MCF-7 were cultured in presence of 5% carbon dioxide at 37 ° C. at doses near the IC50. The viability of cells, inside and outside alkaline phosphatase activity and the amount of total protein in each treatment were studied. The collected data was analyzed with a multivariate analysis of variance (Nested Design) and Dunnett test.
Results: The intracellular alkaline phosphatase activity of the cells showed different behavior compared to the extracellular alkaline phosphatase activity (p< 0.01). The highest increase of alkaline phosphatase activity in two cell lines (K562 and MCF-7) were 339% and 236% which was related to the treatment by macronata daphne.
Conclusion: Unexpected increase in intracellular alkaline phosphatase activity in D. mucronata, D. oleides, Betulin, and Betulinic acid treatment may be due to changes in the composition of plasma membrane component and an increase the non-connected membrane of the protein which is due to the creation of more active proteins.
Keywords: Daphne mucronata, Daphne oleoides, Alkaline Phosphatase, Betulinic Acid, Betulin |
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| Keywords: Daphne mucronata, Daphne oleoides, Alkaline Phosphatase, Betulinic Acid, Betulin |
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Full-Text [PDF 713 kb]
(1184 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2015/05/6 | Accepted: 2015/05/6 | Published: 2015/05/6
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