:: Volume 28, Issue 3 (4-2023) ::
__Armaghane Danesh__ 2023, 28(3): 321-339 Back to browse issues page
Preparation of Topical Cream Containing Sumac (Rhus Coriaria L) Extract and Comparison of its Wound Healing Effects with Phenytoin on Animal Model
M Etemadiyan1 , A Larki Harchgani2 , MR Nooryan2 , M Soleimani Badie3 , R Mahjoub 4, M Mohammadi2
1- Department of Pharmaceutics, Hamedan University of Medical Sciences, Hamedan, Iran
2- Department of Pharmacology and Toxicology, Hamedan University of Medical Sciences, Hamedan, Iran
3- Department of Pharmaceutical Biotechnology, Hamedan University of Medical Sciences, Hamedan, Iran
4- Department of Pharmaceutics, Hamedan University of Medical Sciences, Hamedan, Iran , rmahjub@gmail.com
Abstract:   (1035 Views)
Background & aim: Considering the importance of the skin in regulating the temperature and maintaining body fluids and its prominent role in the defense system, it is important to improve any discontinuity in the skin, which is called a wound. Although limited and superficial wounds heal by themselves, the treatment of deep wounds requires drug therapy. Therefore, the aim of this study was to determine and prepare a topical cream containing the extract of the sumac plant (Rhus Coriaria L) and compare its effect with phenytoin in healing skin wounds in an animal model.

Methods:  In the present study conducted in 2021-2022, twenty-five New Zealand laboratory rabbits, with the weight range of 2500-3000 grams, were obtained from central animal house, Hamadan University of Medical Sciences. The rabbits were randomly divided into five groups: I) Control Group; II) Receiving Phenytoein cream (1%) as the medical therapy; III) Receiving the placebo cream base; IV) Receiving therapeutic cream containing standardized sumac extract (0.1% of tannic acid); and V) Receiving therapeutic cream containing standardized sumac extract (0.05% of tannic acid). Then, a wound, with dimension of 2*2 cm, was induced in the back of any rabbits and the appropriate topical therapy was administered twice daily on the rabbits. The effectiveness of therapy using creams containing sumac extract was determined using the extent of wound healing and the level of hydroxyproline. The obtained data were statistically analyzed using One-way Analysis of Variance (ANOVA).

Results: It was demonstrated that each one gram of standardized extract contained 2.74±0.86 milligram of tannic acid. The data achieved from the study, including the determination of hydroxyl-proline levels, percentage of wound healing, and the duration of remission, demonstrated significant efficiency in treatment with sumac-containing creams (either 0.1% or 0.05%). Histological studies revealed more condensed collagen fibrils and more reduction of inflammation cells in treatment groups receiving sumac-containing cream (either 0.01% or 0.05%) compared with the control group.

Conclusion: Sumac-containing creams, due to anti-oxidative properties and enhancing synthesis of hydroxyl-proline, can condense collagen fibrils which consequently cause improved wound healing.

Keywords: Sumac, Topica cream, Phenytoein, Wound healing, Tannic acid, Animal model.
Full-Text [PDF 926 kb]   (217 Downloads)    
Type of Study: Applicable | Subject: Special
Received: 2022/11/8 | Accepted: 2023/05/14 | Published: 2023/06/6
1. Yu R, Zhang H, Guo B. Conductive biomaterials as bioactive wound dressing for wound healing and skin tissue engineering. Nano-Mciro Letters 2022; 14: 1.## [DOI:10.1007/s40820-021-00751-y] [PMID] []
2. Liang Y, Li M, Yang Y, Qiao L, Xu H, Guo B. pH/ Glucose dual responsive metformin release hydrogel dressings with adhesion and self-healing via dual dynamic bonding for atheltic diabetic foot wound healing. ACS Nano 2022;16(2): 3194-207. ## [DOI:10.1021/acsnano.1c11040] [PMID]
3. Bagheri M, Validi M, Gholipour A, Makvandi P, Sharifi E. Chitosan nanofiber biocomposites for potential wound healing applications: antioxidant activity with synergic antibacterial effect. Bioeng Transl Med 2021; 7(1): 1-15. ## [DOI:10.1002/btm2.10254] [PMID] []
4. Alsamri H, Athamneh K, Pintus G, Eid AH, Iratni R. Pharmacological and antioxidant activities of Rhus coriaria L.(Sumac). Antioxidants 2021; 10(1): 73. ## [DOI:10.3390/antiox10010073] [PMID] []
5. Grassia M, Sarghini F, Bruno M, Cinquanta L, Scognamiglio M, Pacifico S, Fiorentino A, et al. Chemical composition and microencapsulation suitability of sumac (Rhus coriaria L.) fruit extract. Eur Food ResTechnol 2021; 247: 1133-48. ## [DOI:10.1007/s00217-021-03694-1]
6. Vecchio GL, Cicero N, Nava V, Macri A, Gervasi C, Capparucci F, et al. Chemical Characterization, anti-bacterial activity and embryo acute toxicity of Rhus Coriaria L. gentotype from Sicily (Italy). Foods 2022; 11: 538. ## [DOI:10.3390/foods11040538] [PMID] []
7. Elagbar ZA, Shakya AK, Barhoumi LM, Al-Jaber HI. Phytochemical diversity and Pharmacological properties of Rhus Coriaria. Chem Biodiverse 2020; 17(4): e1900561. ## [DOI:10.1002/cbdv.201900561] [PMID]
8. Khalilpour S, Sangiovanni E, Piazza S, Fumagalli M, Beretta G, Delagli M. In vitro evidence of traditional use of Rhus Coriaria L. fruits against skin inflammatory conditions. J Ethnopharmacol 2019; 238: 111829. ## [DOI:10.1016/j.jep.2019.111829] [PMID]
9. Dogan A, Celik I. Healing effect of sumac (Rhus Coriaria) in streptozocin-induced diabetic rats. PharmBiol 2016; 54(10): 2092-102. ## [DOI:10.3109/13880209.2016.1145702] [PMID]
10. Candan F, Sökmen‌ A. Effects of Rhus coriaria L. (Anacardiaceae) on lipid peroxidation and free radical scavenging activity‌. Phytother Res 2004; 18(1): 84-6. ## [DOI:10.1002/ptr.1228] [PMID]
11. Monika P, Chandraprabha M, Rangarajan A, Waiker PV, Murthy KNC. Challenges in healing wound: role of complementary and alternative medicine. Front Nutr 2022; 8: 1198. ## [DOI:10.3389/fnut.2021.791899] [PMID] []
12. Yadolahi-Baghloei M, Pajohi-Alamoti M, Bazargani-Gilani‌ B. Behavior of Staphylococcus aureus Affected by Sumac Water Extract in vitro and Koobideh Kebab. Journal of Food Processing and Preservation 2017; 41(4): e13078. ## [DOI:10.1111/jfpp.13078]
13. Badhani B, Sharma N, Kakkar‌ R. Gallic acid: a versatile antioxidant with promising therapeutic and industrial applications‌. Rsc Advances 2015; 5(35): 27540-57. ## [DOI:10.1039/C5RA01911G]
14. Leyden JJ, Rawlings AV. Skin moisturization. CRC Press; 2002; 547-84. ## [DOI:10.3109/9780203908235]
15. Hemmati AA, Mohammadian F. An investigation into the effects of mucilage of quince seeds on wound healing in rabbit. Journal of Herbs, Spices & Medicinal Plants 2000; 7(4): 41-6. ## [DOI:10.1300/J044v07n04_05]
16. Gabr SA, Alghadir AH. Evaluation of the biological effects of lyophilized hydrophilic extract of Rhus coriaria on myeloperoxidase (MPO) activity, wound healing, and microbial infections of skin wound tissues. Evid-based Complement. Altern Med 2019 (2019). ## [DOI:10.1155/2019/5861537] [PMID] []
17. Cardinal M, Eisenbud DE, Phillips T, Harding K. Early healing rates and wound area measurements are reliable predictor of later complete wound closure. International Journal of Tissue Repair and Generation 2008; 16(1): 19-22. ## [DOI:10.1111/j.1524-475X.2007.00328.x] [PMID]
18. Reddy GK, Enwemeka CS. A simplified method for the analysis of hydroxyproline in biological tissues. Clinical Biochemistry 1996; 29: 225-9. ## [DOI:10.1016/0009-9120(96)00003-6] [PMID]
19. Guvenc A, Koyuncu M. A study on the main active compounds of leaves and fruits of Rhus coriaria L. Turk J Med Sci 1994; 20: 11-3. ##
20. Mavlyanov SM, Islambekov SH, Karimdzhanov AK, Ismailov AI. Anthocyans and organic acids of the fruits of some species of sumac. Chem Nat Compd 1997; 33: 209. ## [DOI:10.1007/BF02291544]
21. Zalacain A, Prodanov M, Carmona M, Alonso GL. Optimization of extraction and identification of gallotannins from sumac leaves. Biosyst Eng 2003; 84(2): 211-6. ## [DOI:10.1016/S1537-5110(02)00246-5]
22. Sissi EL, Ishak HL, El-Wahid MS, El-Ansari MA. The gallotannins of Rhuscoriaria and Mangifera indica. Planta Med 1971; 19: 342-51. ## [DOI:10.1055/s-0028-1099652] [PMID]
23. Zargham H, Zargham R. Tannin extracted from Sumac inhibits vascular smooth muscle cell migration. Mcgill J Med 2008; 11(2): 119-23. ## [DOI:10.26443/mjm.v11i2.601]
24. Alsarayreh AZ, Oran SA, Shakhanbeh JM, Khleifat KM, Al Qaisi YT, Alfarrayeh II, et al. Efficacy of methanolic extracts of some medicinal plants on wound healing in diabetic rats. Heliyon 2022; 8(8): e10071. ## [DOI:10.1016/j.heliyon.2022.e10071] [PMID] []
25. Asai TT, Youshikawa K, Sawada K, Fukamizu K, Koyama Y, Shigemura Y, et al. Mouse skin fibroblasts with mesenchymal stem cell marker P75 neurotropin receptor proliferate in response to prolyl-hydroxyproline. J Funct Food 2020; 66:103792. ## [DOI:10.1016/j.jff.2020.103792]
26. Guo SA, DiPietro LA. Factors affecting wound healing. Journal of Dental Research 2010; 89(3): 219-29. ## [DOI:10.1177/0022034509359125] [PMID] []
27. George Broughton I, Janis JE, Attinger CE. Wound healing: an overview. Plastic and Reconstructive Surgery 2006; 117(7S): 1e-S - 32e-S. ## [DOI:10.1097/01.prs.0000222562.60260.f9] [PMID]
28. Abdulla MA, Fard AA, Sabaratnam V, Wong KH, Kuppusamy UR, Abdullah N. Potential activity of aqueous extract of culinary-medicinal Lion's Mane mushroom, Hericium erinaceus (Bull: Fr.) Pers. (Aphyllophoromycetideae) in accelerating wound healing in rats. Int J Med Mushrooms 2011; 13(1): 33-9 . ## [DOI:10.1615/IntJMedMushr.v13.i1.50] [PMID]
29. Cheng PG, Phan CW, Sabaratnam V, Abdullah N, Abdulla MA, Kuppusamy UR. Polysaccharides-rich extract of ganoderma lucidum (ma curtis: fr.) p. karst accelerates wound healing in streptozotocin-induced diabetic rats. Evid Based Complement Altern Med 2013 (2013). ## [DOI:10.1155/2013/671252] [PMID] []
30. Hajiaghaalipour F, Kanthimathi M, Abdulla MA, Sanusi J. The effect of Camellia sinensis on wound healing potential in an animal model. Evid Based Complement Altern Med 2013 (2013). ## [DOI:10.1155/2013/386734] [PMID] []
31. Amin ZA. Effect of Rhus coriaria extract on wound healing potential in Sprague Dawley rats. Zanco J Med Sci 2018; 22(1): 89-95. ## [DOI:10.15218/zjms.2018.012]
32. Al-Bayati FH, Abdulla MA, Abu Hassan MI, Ali HM. Effect of andrographis paniculata leaf extract on wound healing in rats. Nat Prod Res 2012; 26(5): 423-9. ## [DOI:10.1080/14786419.2010.496114] [PMID]
33. Shukla A, Dubey M, Srivastava R, Srivastava BS. Differential expression of proteins during healing of cutaneous wounds in experimental normal and chronic models. Biochemical and Biophysical Research Communications 1998; 244(2): 434-9. ## [DOI:10.1006/bbrc.1998.8286] [PMID]

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