:: Volume 27, Issue 5 (8-2022) ::
__Armaghane Danesh__ 2022, 27(5): 551-563 Back to browse issues page
Investigating Histological Changes and Gene Expression of Interleukin-8, Interleukin-12, and Interferon Beta in Liver Tissue After Consumption of Lactobacillus rhamnosus GG Probiotic in Steatosis Model Rats
F Rostamkhani
Department of Biology, Yadegar-e-Imam Khomeini(RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran , Shirinrostamkhani@yahoo.com
Abstract:   (459 Views)
Background & aim: Probiotic consumption can regulate the homeostasis of the digestive system and control metabolic diseases related to the digestive system. Therefore, the purpose of the present study was to determine and investigate histological changes and gene expression of interleukin 12, 8 and interferon beta in liver tissue after 5 weeks of consumption of Lactobacillus rhamnosus GG probiotic in steatosis model rats.

Methods: In the present experimental study conducted in 2018, 32 male Wistar rats aged 8 weeks and weighing 180 to 200 grams were included in the study and randomly divided into 4 groups (8 in each group); Control(CON), steatosis(ST), probiotic(PRO) and steatosis + probiotic(ST+PRO) were divided. Rats were modeled by intraperitoneal injection of 140 mg/kg of tetracycline (an antibiotic known to cause steatosis). The value of 107 colony forming units(cfu) of LGG was considered for probiotic groups for 5 weeks (5 days per week, by gavage). Hematoxylin and eosin staining was used to examine histological changes. Also, IL-12, IL-8 and IFN-β gene expression changes were used by RT-PCR method. The collected data were analyzed using one-way analysis of variance statistical software.

Results: Liver tissue histology improved after 5 weeks of probiotic consumption after ST model. ST group showed a significant increase in IL-12, IL-8 and IFN-β gene expression (p<0.05). Compared to the ST group, the PRO and ST+PRO groups showed a significant decrease in IL-12, IL-8 and IFN-β gene expression (p<0.05).

Conclusion: It appeared that the consumption of probiotics is able to improve the cellular and tissue of the liver in people with fatty liver problems by negatively regulating some cellular risk factors. However, further studies are required, especially in human samples.

Keywords:  Hepatic Steatosis, Probiotic, Interleukin, Beta Interferon
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Type of Study: Research | Subject: Physiology
Received: 2022/07/26 | Accepted: 2022/09/12 | Published: 2022/09/13

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