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:: Volume 25, Issue 3 (6-2020) ::
__Armaghane Danesh__ 2020, 25(3): 318-331 Back to browse issues page
The Effect of Aqueous-Alcoholic Aerial Parts Extract of Dorema aucheri on Liposome Nanoparticles on Proliferation and Induction of Apoptosis in Human Colorectal Cancer Cells of SW48 Class
Abstract:   (2951 Views)
Background & aim: Nowadays, plant extracts are used for research and clinical application in the treatment of cancers including colorectal cancer. Dorema aucheri plant has been conventionally used as an herbal medicine and its extract has been reported to contain significant amounts of antioxidants such as; Flavonoids, anthocyanins and phenolic acid. Therefore, the aim of the present study was to determine the effect of aqueous-alcoholic aerial parts extract of Dorema aucheri shoot on liposome nanoparticles on proliferation and induction of apoptosis in SW48 human colorectal cancer cell line.
 
Methods: The present experimental study was conducted in 2019 at Yasuj University of Medical Sciences. SW48 cells were cultured in vitro, and then treated with different concentrations of Dorema aucheri hydroalcoholic extract, Dorema aucheri -loaded nanoliposomes, liposomes alone for 24, 48, and 72 hours. The cytotoxic effect was assessed using MTT assay. Synthesis of nanoliposomes was formulated by lipid thin film filtration method. Apoptosis was measured by staining cells with propidium iodide and annexin V using flow cytometry. Vincristine was used as a positive control in the study. The collected data were analyzed using two-way ANOVA and Tukey's post hoc test. b
 
Results: The results of 50% inhibitory concentration (IC50) of Dorema aucheri  nanoparticles and Dorema aucheri plant extract in 24 hours were 33.48 and 44.67 μg / ml, respectively, and for vincristine 50 ng / ml. The results indicated that the survival rate of cells was inhibited in a dose and time-dependent manner. The results of apoptosis test displayed that the highest percentage of increase in apoptosis in this cell line compared to the control was related to Dorema aucheri nanoliposomes (41.5%). On the other hand, Dorema aucheri extract alone had less increase than the control(p <0.05).
 
Conclusion: The results of te present study revealed that loading Dorema aucheri extract in nanoliposomes increased the toxicity and apoptosis effect of Dorema aucheri extract at low doses and could be a worthy candidate with less side effects for more extensive in vitro research.



 
Keywords: Dorema aucheri, Apoptosis, Nanoliposome, SW48
Full-Text [PDF 1965 kb]   (765 Downloads)    
Type of Study: Research | Subject: Biochemistry
Received: 2020/02/15 | Accepted: 2020/06/30 | Published: 2020/07/7
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The Effect of Aqueous-Alcoholic Aerial Parts Extract of Dorema aucheri on Liposome Nanoparticles on Proliferation and Induction of Apoptosis in Human Colorectal Cancer Cells of SW48 Class. armaghanj 2020; 25 (3) :318-331
URL: http://armaghanj.yums.ac.ir/article-1-2726-en.html


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Volume 25, Issue 3 (6-2020) Back to browse issues page
ارمغان دانش Armaghane Danesh
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