[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
Indexing & Abstracting::
Publication Ethics::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Indexing & Abstracting
DOAJ
GOOGLE SCHOLAR
..
:: Volume 25, Issue 1 (3-2020) ::
__Armaghane Danesh__ 2020, 25(1): 12-22 Back to browse issues page
Effect of the Protective Role of Long-Term Ischemic Preconditioning on Acute Renal Impairment Due to Re-Ischemic Resection Through TLR-4 and TNF-α Signaling Pathway in Rats
F Gholampour1 , J Roozbeh2 , S Janfashan3 , L Malek Mahal2 , K Rahimi Jaberi2 , Z Karimi 4
1- Department of Biology, Shiraz University, Shiraz, Iran
2- Clinical Disease Research Centers, Shiraz University of Medical Sciences, Shiraz, Iran
3- Department of Biology, Zarghan Branch, Islamic Azad University, Zarghan, Iran
4- Clinical Disease Research Centers, Shiraz University of Medical Sciences, Shiraz, Iran , zkarimi@sums.ac.ir
Abstract:   (4246 Views)
Background & aim: Acute Kidney Injury (AKI) is an inflammatory process in which multiple inflammatory factors are involved. Recently, one of the ways to alleviate inflammation in AKI is to apply pre-conditioned ischemic remote control (RIPerC). The aim of the present study was to determine and investigate the protective role of long-term ischemic preconditioning in acute injury of all ischemia due to resection through the TLR-4 and TNF-α signaling pathways in the rat.
 
Methods: The present experimental study was conducted in 2016 on 30 male rats of Sprag Dolly breed with a weight range of 250 to 280 grams. Ratings were divided into three equal groups of control, re-ischemic resection (I / R) and re-ischemic resection with long-term ischemic preconditioning (RIPerC) predisposition. In this study, I / R re-bleeding was observed with bilateral closure of the artery and renal vein for 45 minutes and 24 hours. The RIPerC model consisted of four five-minute cycles (two minutes for closing the left femoral artery and three minutes for re-bleeding) at the onset of ischemic-renal failure. At the end of the resuscitation period, urine, blood, and kidney tissue samples were collected for functional, structural, and molecular analysis. The collected data were analyzed using SPSS software.
 
Results: Ischemia-reperfusion caused tissue damage and subsequently impaired renal function, which was demonstrated by a decrease in creatinine clearance and an increase in its relative sodium excretion. In addition, in the I/R group, mRNA level of TLR-4 and TNF-α in tissue increased, whereas RIPerC (in this group, during ischemia, ischemia, and reperfusion in the femoral artery was done) simultaneously with I/R improved kidney structure and function and decreased expression of TLR-4 and TNF-α.
 
Conclusion: RIPerC protected the kidney against ischemia-reperfusion-induced kidney injury and probably this protective effect was exerted by inhibition of TLR-4 signaling pathway in the kidney.
 
 
Keywords: Ischemia-renal reperfusion, Remote ischemic per conditioning, Toll like receptor-4, TNF-α, kidney inflammation, creatinine clearance
Full-Text [PDF 1741 kb]   (575 Downloads)    
Type of Study: Research | Subject: Physiology
Received: 2019/08/28 | Accepted: 2020/02/17 | Published: 2020/03/4
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Gholampour F, Roozbeh J, Janfashan S, Malek Mahal L, Rahimi Jaberi K, Karimi Z. Effect of the Protective Role of Long-Term Ischemic Preconditioning on Acute Renal Impairment Due to Re-Ischemic Resection Through TLR-4 and TNF-α Signaling Pathway in Rats. armaghanj 2020; 25 (1) :12-22
URL: http://armaghanj.yums.ac.ir/article-1-2607-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 25, Issue 1 (3-2020) Back to browse issues page
ارمغان دانش Armaghane Danesh
Persian site map - English site map - Created in 0.05 seconds with 39 queries by YEKTAWEB 4645