:: Volume 24, Issue 5 And ضمیمه2 (12-2019) ::
__Armaghane Danesh__ 2019, 24(5 And ضمیمه2): 935-949 Back to browse issues page
The Effect of Resistance Training on the Expression of Cardiac Muscle Growth Regulator Messenger Genes in Obese Male Rats
A Moini1 , S Farsi 2, SA Hoseini3 , M Mehrzad4
1- Department of Physical Education and Sport Sciences, Larestan Branch, Islamic Azad University, Shiraz, Iran,
2- Department of Physical Education and Sport Sciences, Larestan Branch, Islamic Azad University, Shiraz, Iran, , Sirous.farsi@gmail.com
3- Department of Exercises Physiology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran
4- Department of Physical Education, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
Abstract:   (2738 Views)
Background & aim: Obesity is associated with cardiovascular disease and may lead to pathological cardiac hypertrophy, but physical activity (resistance training) modulates some intracellular signaling pathways associated with the regulation of hypertrophy. The aim of the present study was to determine and evaluate the effect of resistance training on the expression of cardiovascular regulatory genes in obese male rats.
Methods: In the present experimental study which was conducted in 2016, 18 male Sprague Dawley rats were divided into 3 groups of healthy control, obesity control and resistance training, after eight weeks of using high fat diet. The rats were dissected 48 hours after exercise protocol and their heart muscle was separated. The expression of genes (AMPK, mTOR, S6K, 4EBP, COL1, COL3, AngII) was determined by Real time-PCR technique. The amount of transgene was calculated by CT-2ΔΔ method. Data were analyzed by statistical tests. Independent t-test, Kolmogorov-Smirnov and one-way ANOVA were used for data analysis.
Results: The results indicated that the resistance training group compared to the control group showed an increase in expression of mTOR(p=0.003), 4EBP (p=0.004), which was not statistically significant. On the other hand, expression of S6K (p=0.002) AMPK (p=0.008) increased significantly. Also, there was a decrease in the expression of collagen 1 (p=0.003), collagen 3 (p=0.001) Ang II (p=0.001) which were statistically significant.
Conclusion: Obesity in rats seemed to induce cardiac pathological hypertrophy and accelerate the process of cardiac senescence, but resistance training, which was a suitable replacement, partially reduced the negative effects of obesity on cardiac function in the process of cardiac senescence.
Keywords: Cardiac muscle, Obesity, Resistance Training.
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Type of Study: Research | Subject: Sport Physiology
Received: 2019/07/13 | Accepted: 2019/09/15 | Published: 2019/12/24

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Volume 24, Issue 5 And ضمیمه2 (12-2019) Back to browse issues page