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Comparison of the Toxic Effects of Hydroalcoholic Extract of Glaucium flavum on Glioblastoma and Non-Cancer Nerve Cells
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E Hoveizi    1, M Kalantari2 , K Hushmandi3 |
1- Stem cells and transgenic technology research center (STTRC), Shahid Chamran University of Ahvaz, Ahvaz, Iran, , e.hoveizi@yahoo.com
2- Department of Modern Science, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
3- Department of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran |
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Abstract: (3503 Views) |
Background & aim: Glioblastoma tumors are one of the most invasive brain tumors of neural tissue origin. The low success rates of these types of tumors require the search for new treatment options. Glaucium flavum is widely used in the pharmaceutical industry because of its high content of alkaloids. The aim of this study was to determine and evaluate the toxic effects of hydroalcoholic extract of Glaucium flavum glioblastoma and non-cancerous nerve cells.
Methods: the present research was an experimental study carried out in the cell culture research laboratory of Shahid Chamran University of Ahvaz in 2019. A pregnant rat was used in this study for cell isolation. The Glaucium flavum extract was first extracted and nerve progenitor cells then extracted from the brain cortex of 17-day-old pregnant mouse embryos by enzymatic digestion. Cells were then treated with Glaucium flavum at concentrations of 100, 300, 500, and 1000 μg/ml for 24 and 72 h. After treatment, cell viability was assessed by MTT photometric method and cell morphology was evaluated by DAPI fluorescent staining. Data were analyzed using one-way ANOVA.
Results: Morphological changes were observed in the cells treated with IC50. Evaluation of cell survival by MTT assay showed that the Glaucium flayum extract significantly decreased the survival of C118 cells as well as neural neurospheres (NCs) at both 24 and 72 h intervals. The severity of Glaucium flayum extract cytotoxicity on glioblastoma cells was higher than the neurospheres and showed a significant difference (p <0.05). The results of cell viability also showed that the concentration of 300 and 500 μg/ml of the Glaucium flayum extract was IC50 for C118 and NCs cells respectively.
Conclusion: It was concluded that natural products such as Glaucium flayum extract with pharmacological and biochemical properties had antioxidant and anti-inflammatory properties and have less adverse effects on non-cancerous brain cells than in non-cancerous cells.
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| Keywords: Glioblastoma, Glacium flavum, Neuronal precursor cell, Cell viability |
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Full-Text [PDF 3578 kb]
(586 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2019/05/10 | Accepted: 2019/09/15 | Published: 2020/01/12
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