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Frequency of CTX-M-1 Gene in Escherichia coli Isolates of ESBL-Producing Enzyme in Clinical Samples by Polymerase Chain Reaction (PCR) in Yasuj
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Z Khishvand1   , M Hosseini2 , A Sharifi1 , SM Tabatabaei3 , SAM Khosravani 4 |
1- Cellular and Molecular Research Center, yasuj University of Medical Sciences, yasuj, Iran
2- Student Research Committee, yasuj University of Medical Sciences, yasuj, Iran
3- Women's group,yasuj University of Medical Sciences,yasuj, Iran
4- Cellular and Molecular Research Center, yasuj University of Medical Sciences, yasuj, Iran , Khosravani2us@yahoo.com |
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Abstract: (3084 Views) |
Background & aim: Resistance to β-lactam antibiotics in the clinical isolates, in most cases is caused by β-lactamase enzymes. In recent years, The incidence of broad-spectrum β-lactamase enzymes (ESBLs) among clinical isolates especially E.coli is greatly increased, since the β-lactamase have several subfamilies, using universal primers designed to detect the following complete families could be useful. β-lactamase producing enzymes (ESBLs) of E. coli has created many problems for patients. β-lactamase CTX-M-1 gene is the cause of resistance. The aim of this study was to evaluate CTX-M-1gene in E.coli.
Methods: In this practical study, susceptibility of isolated bacteria to 13 antibiotics were indicated by disk diffusion method according to CLSI guidelines and strains were analyzed for the presence of widespread β-lactamase enzymes via two-disc synergy method. Thus، the prevalence of CTX-M1 ESBL gene samples were determined using PCR and the data were analyzed using ANOVA.
Results: A total of 200 isolates of E.coli were isolated. The presence of CTX-M-1 gene were also isolated using the PCR method. From 200 strains studied, 62 (31%), of strains produced ESBL. After PCR processing of 62 produced ESBL, 43 isolates (69.4%) were identified as CTX-M-1 genes. Also, antibiotic susceptibility test showed the highest percentage of resistance to Cotrimoxazole antibiotic (50%) and the lowest antibiotic resistance to imipenem (0%).
Conclusion: The results of this study showed the high percentage of β-lactamase resistance among of E.coli strains. This is a serious public hazard that should be pointed out to measures for preventing this hazard. Considering the sensitivity of the studied beta-lactam resistant isolates and isolates in Iran to imipenem, a carbapenem with a non-beta-lactam antibiotic is recommended for the treatment of nosocomial infections caused by these strains.
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| Keywords: E.coli, ESBL, PCR, CTXM1 |
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Full-Text [PDF 1478 kb]
(559 Downloads)
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Type of Study: Research |
Subject:
Microbiology
Received: 2018/02/3 | Accepted: 2019/12/15 | Published: 2020/01/12
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