[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Contact us::
Site Facilities::
Indexing & Abstracting::
Publication Ethics::
Search in website

Advanced Search
Receive site information
Enter your Email in the following box to receive the site news and information.
Indexing & Abstracting
:: Volume 28, Issue 4 (7-2023) ::
__Armaghane Danesh__ 2023, 28(4): 474-490 Back to browse issues page
The Effects of Oak Placenta(jaft) Extract Combined with Cisplatin on Gastric Cancer Cell Line(AGS)
S Andarzi1 , H Sadeghi2 , SM Syyednejad1 , MR Hajari 3
1- Department of biology, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2- Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
3- Department of biology, Shahid Chamran University of Ahvaz, Ahvaz, Iran , Mohamad.hajari@gmail.com
Abstract:   (544 Views)
Background & aim: Oak placenta(Jaft) extract has potent antioxidant, anti-proliferative, anti-cancer activity, and other therapeutic properties. Therefore, the aim of this study is to investigate the effect of Jaft extract and its combination with Cisplatin on gastric cancer adenocarcinoma cell line (AGS).

Methods: In the present experimental study conducted at Shahid Chamran University of Ahvaz in 2022, the AGS cell line was cultured in 1640-RPMI medium and treated with Jaft extract and cisplatin. Cell viability and combination index(CI) were measured using the MTT assay and Compucyn software(respectively). Moreover, the expression of the CDK2 gene as an important gene involved in the cell cycle was evaluated by Real-Time PCR. The collected data were analyzed using Graph Pad Prism 6 software and one-way ANOVA.

Results: The findings of the present study indicated that Jaft extract and cisplatin cause decreased cell viability of AGS (p< 0.0001). The analysis of compusyn data indicated that these two substances together have synergistic properties (CI<1), in addition to simultaneous usage of Jaft extract increases the cytotoxicity of cisplatin drug. In the simultaneous treatment, it was observed that the expression of CDK2 decreased and led to an increase in the anticancer effect of cisplatin (p<0.001).

Conclusion: The present study revealed that placenta extract had anti-proliferative and anti-cancer properties. According to the synergistic effects of these two drugs, Jaft can change the gene expression of CDK2 in the cell cycle and introduce a possible approach for the treatment of gastric cancer with more potency and less dose of administered cisplatin to induce toxicity. The results of the present study indicated that Jaft extract is a proper selection to sensitize cancer cells to cisplatin.

Keywords: Jaft extract, Cisplatin, AGS cell line, Adenocarcinoma, CDK2
Full-Text [PDF 606 kb]   (77 Downloads)    
Type of Study: Research | Subject: Internist
Received: 2023/01/23 | Accepted: 2023/06/20 | Published: 2023/07/10
Send email to the article author

Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Andarzi S, Sadeghi H, Syyednejad S, Hajari M. The Effects of Oak Placenta(jaft) Extract Combined with Cisplatin on Gastric Cancer Cell Line(AGS). armaghanj 2023; 28 (4) :474-490
URL: http://armaghanj.yums.ac.ir/article-1-3429-en.html

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 28, Issue 4 (7-2023) Back to browse issues page
ارمغان دانش Armaghane Danesh
Persian site map - English site map - Created in 0.05 seconds with 38 queries by YEKTAWEB 4624