2024-03-28T12:52:48+03:30 http://armaghanj.yums.ac.ir/browse.php?mag_id=47&slc_lang=en&sid=1
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Armaghane Danesh armaghanj 1728-6506 1728-6514 10.61186/armaghanj 2010 14 4 Isolation, Culture and Differentiation of Chicken Bone Marrow Mesenchymal Stem Cells M Kadivar kadivar@pasteur.ac.ir F Piryaei M Ramezani Introduction & Objective: At the present time, the importance of stem cells, especially mesenchymal stem cells, is of utmost importance for scientists. Mesenchymal stem cells are adult stem cells which can divide into a variety of different cells. Regarding this, it is of importance in preclinical studies. The aim of this study was the isolating of mesenchymal stem cells derived from chicken bone marrow and assessing their ability for differentiation. These cells can be used in the studies related to tissue engineering and gene therapy. Materials & Methods: This was an experimental study which was conducted at Pasteur institute of Iran in 2008-2009. In this study, 15-day old Raf chickens were used. For cultivating the mesenchymal stem cells, bone marrows of the legs and tibia were extracted with flashing technique. After the cell cultivation and proliferation, a part of the cells was frozen in liquid nitrogen. In order to make sure that these cells were mesenchymal stem cells. The cells were differentiated into three lines of bone, cartilage and adipose. Specific staining was done for histological analysis. Results: Chicken bone marrow is a potential source of adult stem cells. Stem cells derived from bone marrow could differentiate to Osteocyte, Chondrocyte and Adipocyte cells. Conclusion: For the first time, results of this research indicated that stem cells derived from chicken’s bone marrow are an important source of stem cells. They have the potential for differentiation are cost effective, have a simple isolating method and are free of bioethical problems. Stem cells Cell differentiation Chicken Bone Marrow 2010 1 01 1 11 http://armaghanj.yums.ac.ir/article-1-575-en.pdf
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Armaghane Danesh armaghanj 1728-6506 1728-6514 10.61186/armaghanj 2010 14 4 Evaluation of Touchdown Nested PCR to Circumvent Spurious Priming and Increase Specificity during HIV and GBV-C Gene Amplification SH Falahi M Ravanshad ‌Ravanshad@modares.ac.ir O Kenarkohi R Ghanbari M Haji Abdolbaghi Introduction & Objective: Primer-Template hybridization temperature is one of the important parameters in Nested PCR optimization. Unlike instant temperature for sequence amplification in routine PCR process, Touchdown PCR is a modified form of standard PCR that employs a range of annealing temperature. This study intended to develop a Touchdown Nested PCR in order to circumvent spurious priming and enhancing specify during gene amplification. Materials & Methods: This is an experimental study conducted at Tarbiat Modarres University of Tehran during 2008-2009. Study samples were collected from Digestive Diseases Research Centre- at Shari'ati Hospital and HIV research center – Imam Khomeini Hospital. After extracting the nucleic acid, primer designing for HIV and GBV-C and c-DNA synthesis Nested PCR was performed on negative and positive samples using standard and touchdown protocols. Results: The intended band was observed in all positive samples. No band was observed in any human and viral negative control samples. After electrophoresis of PCR products, non specific band were seen in HIV and GBV-C samples during standard PCR. Using the touchdown protocol, undesirable bands were omitted or significantly decreased. Conclusion: In the present study, despite the formation of uncalled bands in standard reaction using the touchdown method led to omission of non-specific bands without any significant effect on the final products. As for its simplicity, cost and time saving, it seems that using this method is a rational and economical way for fast optimization of PCR reactions. Touchdown Nested PCR spurious priming specificity gene amplification 2010 1 01 12 22 http://armaghanj.yums.ac.ir/article-1-577-en.pdf
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Armaghane Danesh armaghanj 1728-6506 1728-6514 10.61186/armaghanj 2010 14 4 Evaluation of Hydro-alcoholic Extract of Peganum harmala on Pituitary-thyroid Hormones in Adult Male Rats E HOssini Ebrahim.hossini@yahoo.com H Sadeghi A Daneshi Introduction & Objectives: Peganum harmala from the Jigo Phalluses family has compounds such as: alkaloid,saponine steroid and lignin which is used as a traditional medicine witht antibacterial, anti tumor, inhibition of MAO enzyme, and stimulation of the nerve system. It also serves as a modulator to endocrine activities. The aim of the present study was to evaluate the effect of the hydro-alcoholic extract of Peganum harmala on plasma levels of pituitary-thyroid’s hormones of adult rats. Materials & Methods: In this experimental study, which was conducted at Yasuj University of Medical sciences in 2009, 50 adult Mala rats with the approximate weight of 260+30 grams were divided into 5 groups: the control group, the sham group, and 3 experimental groups. The control group did not take any medicine. The sham group received 1 mL of distilled water daily for 17 consecutive days. The experimental groups took 90 mg/kg, 180mg/kg, or 270 mg/kg of Peganum harmala extract daily respectively for 17 consecutive days. In the 18th day, by collecting the blood samples of the animals, plasma level of TSH, T4, and T3 was measured using radioimmunoassay method. Collected data were analyzed using SPSS software. Results: This study revealed that the minimum and maximum dose of the Peganum harmala extract reduces the TSH level and average and maximum dose of the extract significantly reduces the level of T4 and T3 in rats. Conclusion: results of this study indicate that by further study the Peganum harmala extract might be used for treatment hyperthyroidism. However further study is needed to explore this concept. TSH T4 T3 Peganum Harmala 2010 1 01 23 30 http://armaghanj.yums.ac.ir/article-1-579-en.pdf
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Armaghane Danesh armaghanj 1728-6506 1728-6514 10.61186/armaghanj 2010 14 4 DFNB59 Gene Mutations and its Association with Deafness in Schoolchildren in Kohgilooyeh & Boyerahmad Province M Taherzadeh Ghahfarrokhi E Farrokhi A Shirmardi S Ghasemi M Abolhasani F Azadegan S Reisi M Reisi G Banitalebi M Hashemzadeh Chaleshtori Introduction & Objective: Hearing loss is a common disease affecting millions of people worldwide. Hearing loss can be caused due to genetic or environmental factors or even both. The genetic of hearing defect is highly heterogeneous and more than 100 genes are predicted to cause this disorder in humans. A newly identified gene (DFNB59) has been shown to cause deafness in some populations. Here we report mutation analysis for DFNB59 gene in 88 genetic non-syndromic hearing loss subjects. Materials & Methods: In this descriptive-lab based study which was conducted at the Cellular and Molecular Research Center of Shahrekord University of Medical Sciences, DNA was extracted from the peripheral blood samples using standard phenol chloroform procedure. Mutation analysis for DFNB59 gene was performed using PCR-SSCP/HA protocol. The suspected DFNB59 which was detected as shifted bands on PAGE were then confirmed by direct sequencing strategy. Results: Two DFNB59 polymorphisms including c.793C>G and c.793C>T were detected in 8 and 1 deaf subjects respectively. Conclusion: We conclude that there is no association between DFNB59 mutations and deafness in the studied patients in the region. Deafness DFNB59 PCR-SSCP Heteroduplex Analysis. 2010 1 01 31 39 http://armaghanj.yums.ac.ir/article-1-584-en.pdf
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Armaghane Danesh armaghanj 1728-6506 1728-6514 10.61186/armaghanj 2010 14 4 Determining the Effect of Zizphus Jujba Extract Together with Phototherapy in Reducing Bilirubin Concentration in Neonatal Jaundice S Ebrahimi A Pourmahmoudi Azizpourmahmoodi@Yahoo.com M Mohhammadhosini H Nasrolahi S Ashkani Introduction & Objective: Neonatal jaundice is observed in the first week of birth among 60% of mature and 80% of immature neonates. This epidemic puts a lot of cost on the society. Today, phototherapy is used for the treatment of neonatal jaundice. Reducing the treatment period decreases the hospitalization costs. The Purpose of this study was to determine the effect of Zizphus Jujba extract and phototherapy on reducing both Bilirubin concentration and also the hospitalization period of neonatal jaundice patients. Materials & Methods: This was a clinical-trial study which was conducted at Imam Sajjad Hospital of Yasuj in 2009. 121 of the hospitalized neonates were chosen and divided into 2 groups: case group (63 neonates) and control group (58 neonates). 1 cc of the Zizphus Jujba extract per each kilogram of body weight was orally given three times a day to the case group. Also phototherapy was used in the case group. Phototherapy was the only treatment used for the control group. The Bilirubin concentration of the hospitalized patients was measured two times a day in the hospitalization period. The collected data was analyzed by the SPSS software using t-test. Results: The mean and standard deviation of the Bilirubin concentration in the control group before the intervention were 20.9±2.3 milligrams per 100 and after intervention was 12.27±1.23 milligrams per 100 while in the case group before intervention was 22.3±0.05 and after intervention was 12.27±1.01 milligrams per hundred. A significant difference was seen between the case and control group before and after intervention (p=0.001. Moreover, a meaningful reduction of the hospitalized days and a meaningful increase of excrement and urine in the case group were observed. Conclusion: This study revealed that administration of Zizphus Jujba extract with increasing amount of excrement and urine of the neonates can lead to Bilirubin discharge and can be effective on curing neonatal jaundice. Zizphus Jujba neonates Bilirubin jaundice 2010 1 01 40 47 http://armaghanj.yums.ac.ir/article-1-585-en.pdf
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Armaghane Danesh armaghanj 1728-6506 1728-6514 10.61186/armaghanj 2010 14 4 Evaluating the Protective Effects of Melissa Officinalis on Learning Deficits of Rat’s Offspring Exposed to Lead Acetate During Pre- and Postnatal Periods Z Momeni S Rostami N Ghayour M Behnam Rassouli behnam@ferdowsi.um.ac.ir Introduction & Objective: Lead contamination dramatically influences different body systems especially the central nervous system. Lead absorption during gestational period has deleterious effects on fetal differentiation and development and it may possibly result in learning deficits in adulthood. Recent studies have demonstrated positive effects of Melissa officinalis on memory improvement in some neural disorders. The aim of the present study is to investigate the protective effects of Melissa on learning deficits in lead acetate exposed rats. Materials & Methods: In this experimental study in department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad (2008-2009), 40 mated Wistar rats were divided into 8 groups as follows: control, negative control (Pb), Melissa (M) and Pb+M, and each in 3 different subgroups. The treatment started from the 7th day of gestation and continued during pregnancy and lactation. The learning ability and memory retention of four months old offspring were tested by complex T-maze. The collected data was analyzed by the SPSS software using one-way ANOVA and Toki test. Results: A significant difference was found between lead exposed group and other groups regarding the time to reach the goal and the number of errors while there was no meaningful difference between the control and other experimental groups. Conclusion: In lead exposed rats, learning deficits were obviously noticed. Since there was meaningful difference between control and Pb+M subgroups, Melissa can possibly improve learning deficits in lead acetate exposed rats. Melissa Officinalis Learning deficits Lead acetate Rat Complex T-maze 2010 1 01 48 57 http://armaghanj.yums.ac.ir/article-1-586-en.pdf
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Armaghane Danesh armaghanj 1728-6506 1728-6514 10.61186/armaghanj 2010 14 4 The Effect of Silybum marianum on GFAP and Spatial Memory in a Mouse Model of Alzheimer\'s Disease A Hadinia R Aryanpour Roya_Aryan@yahoo.com M Mehdizadeh R Mahmodi A Mossavizadeh H Delaviz H Pirhajati A Ghnbari Introduction & Objective: Studies have shown that Silybum marianum have high levels of antioxidant polyphenolic substances and have neuro-protective effects on neurodegenerative diseases. Accordingly, this study was conducted to determine the possible effect of Silybum marianum on expression of and spatial memory in a mouse model of Alzheimer's disease. Materials & Methods: This experimental study was conducted at Yasuj University of Medical Sciences in 2009. Thirty adult male Wistar rats were allocated in three groups: sham group, experimental group, and lesion group, each consisting of ten rats. The experimental and lesion groups received Ibotonic acid of the NBM nucleus in stereotaxic apparatus whereas the sham group underwent surgical procedure without any injection. The experimental group received 200mg/kg of Silybum mirianum extract orally, diluted in 1% Arabic gum. Also the sham group received 1% Arabic gum every day for four weeks. The lesion group did not receive anything. The behavioral assessment was measured, after treatment , by using of Y maze test on day 7 and 28 in all groups. The ELISA method was used to measure the GFAP level in Hippocamp at the end of behavioral assessment. The collected data was analyzed by the SPSS software using ANOVA and Repeated Measures of Analysis Variance tests. Results:Improvement of behavioral performance of the experimental animals compared to the lesion and sham groups were increased significantly on day 7 and 28 (P <0.01 & P <0.001 respectively). The ELISA method showed that the level of the GFAP synthesis decreased in the experimental group compared to the lesion and sham groups (P <0.001). Conclusion: The Silybum marianum plant has a protective effect on the nerve tissue in a mouse model of Alzheimer's disease by decreasing of the GFAP synthesis and lead to the improvement of behavioral performance. : Alzheimer\'s Disease Spatial Memory Silybum Marianum 2010 1 01 65 75 http://armaghanj.yums.ac.ir/article-1-588-en.pdf
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Armaghane Danesh armaghanj 1728-6506 1728-6514 10.61186/armaghanj 2010 14 4 Determining the Most Suitable Spirometric Parameters to Differentiate Chronic Obstructive pulmonary Disease (COPD) from Asthma S Mehrabi MR Sadin sadinmr @sums.ac.ir SMA Ghauomi Introduction & Objective: Chronic Obstructive Pulmonary Disease (COPD) and asthma are common diseases with difference in prognosis and treatment. It is believed that spirometry is the best modality for differentiating the two conditions however, the data is heterogeneous. This study aims at determining the most suitable spirometric parameters in this regard. Materials & Methods: In this case control study, fifty patients (25 patients with asthma and 25 patients with COPD) in pulmonology ward of Dr.Faqi’hi Hospital of Shiraz in 2008 under the base spirometry and post bronchodilator were recruited and comparison between these two groups was done. The collected data was analyzed by the SPSS software using independent t-test, Man-Whitney, Chi-square and Fisher test. Results: Frequency of male patients and the mean age were significantly higher in the COPD group. Increase of forced expiratory volume in one second (FEV1) after administration of bronchodilator was significantly higher in asthmatic patients with an optimal cut-off point of ≥0.165 L (15% increase) yielded a sensitivity and specificity of 64% and 60%, respectively. Change of other parameters was not significantly different between the two groups. The best differentiating spirometric parameter was post-inhalation FEV1/predicted value with an optimal cut-off point of ≥64.2, with relating sensitivity and specificity of 88%. Conclusion: According to our results and in concordance to other reports, changing of spirometric parameters after inhalation of a short-acting bronchodilator is not a reliable indicator of asthma or COPD. Post-inhalation FEV1/predicted value is the best parameter in this regard however, the clinical suspicion should not be replaced by it. Asthma COPD Spirometry 2010 1 01 76 87 http://armaghanj.yums.ac.ir/article-1-589-en.pdf
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Armaghane Danesh armaghanj 1728-6506 1728-6514 10.61186/armaghanj 2010 14 4 A Case Report of Radial Artery Spasm during Anesthesia M Masoudifar dr122m@yahoo.com F Rouhani Introduction & objective: One of the most important drawbacks of radial artery is its tendency toward spasm and one of the rare etiologies of that is recurrent blood sampling of the artery. Other causes are injection of drugs in artery in spite of vein, or using radial artery in cardiopulmonary bypass. Papaverine is a useful drug in vasospasm, but it must be used with special caution to avoid it's complications such as arrhythmia. Case: The patient was a 72 year old man with intestinal gangrene and peritonitis whom was operated in Al-Zahra hospital in 2008. During Laparatomy surgery, because of severe acidosis, blood sampling was done 3 times for blood gas analysis. After being taken to the recovery ward, no radial pulse was detectable in the patient's right hand and severe cyanosis in the same hand was seen. After inserting the arterial catheter in brachial artery, treatment with Papaverine, indications of recovery were seen. Conclusion: Despite the rarity of radial artery spasm during anesthesia, it is very dangerous situation, and anesthesiologist must try to prohibit and be able to manage this problem and have suitable drugs in the operation room. Spasm Radial artery Papaverin 2010 1 01 88 93 http://armaghanj.yums.ac.ir/article-1-590-en.pdf
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Armaghane Danesh armaghanj 1728-6506 1728-6514 10.61186/armaghanj 2010 14 4 Determination of the Effect of Cognitive Behavioral Therapy on Stress among Nurses at Yasuj Shahid Beheshti Hospital in 2009 SH ShahAbadi mzh541@yahoo.com SHA Kharamin MR Firozi SA HoosiniMotlagh M Malekzadeh Introduction & Objective: Stress is one of the most important phenomena in this century that has had a great impact on people’s mental and physical health and has become one of the main topics of organizational management. Primarily, nursing and taking care of patients is stressful itself. Therefore, in the working environment, any menace to organizational goals and reduction in the quality of nursing cares are due to stress. There are different therapeutic ways to deal with stress which cognitive behavioral therapy is one of them. The goal of this study was to examine the effects of cognitive behavior therapy on stress among nurses who worked at Yasuj Shahid Beheshti hospital in 2009. Materials & Methods: This was an experimental research that was carried out among 100 nurses working at Yasuj Shahid Beheshti hospital in 2009. The sampling type was available samples. After filling the DASS-42 questionnaire, we chose 30 cases that had the worst status from point of stress and were randomly divided into two groups: case and control groups. Each group consisted of 15 nurses. The cases were trained in 8 weeks, an hour a week, by a single clinical psychologist specialized in cognitive behavioral therapy. After the therapy, questionnaires were filled again by the two groups. Finally, two groups were compared together in view point of stress scores. For analysis of data we used the SPSS software and descriptive statistics, t-test and ANOVA. Results: The mean and standard deviations of stress in case group on the pre-test were7.96±18.73 and on the post test were 6.295±12.266 respectively, the mean and standard deviations of stress in the control group for pre-test were 8.413±21.066 and for post test were 9.019±20.733 respectively. There weren’t any significant differences between two groups before the intervention, but there were significant differences between case groups after the intervention. (p < 0.05). Conclusion: According to the findings of the study, cognitive behavioral techniques' training significantly reduces stress in nurses. Therefore, it is recommended to reduce nurses’ stress and increase the level of nursing services and promoting patients’ satisfaction by establishing a psychology consulting unit in hospitals. nurses stress cognitive behavior therapy 2010 1 01 580 64