1 1728-6506 Yasuj University Of Medical Sciences 1394 Immunology The Change in the Functions of Neutrophils after Being Stimulated with LPS-primed Mesenchymal Stem and Treated with Green Tea Abbasi A b Rahmani Kukia N c , Abtahi Froushani SM d b Department of Microbiology, Urmia University, Urmia, Iran c Department of Biochemistry, Ardabil University of Medical Sciences, Ardabil, Iran d Department of Microbiology, Urmia University, Urmia, Iran 1 9 2016 21 6 521 535 27 06 2016 19 08 2016 Background & aim: Recently, the interaction of lipopolysaccharide activated by mesenchymal stem cells and neutrophils has been proven. The aim of the present study was to evaluate the effect(s) of the Green tea extract, as a widely consumed beverage on the interaction of lipopolysaccharide activated MSCs on neutrophils. Methods: In the present experimental study, after the isolation of mesenchymal stem cells from bone marrow of rats was conducted, these cells were stimulated with 10 ng/mL LPS for 1. Then, the supernatant was discarded and washed out to remove LPS cells.  Afterwards, the cells were incubated with different concentrations of green tea (10, 100 and 500 micrograms per ml) for 24 hours. Subsequently, the mesenchymal stem cells were co-cultured with neutrophils and incubated for other 4 h. Finally, the neutrophil function was evaluated. The data were analyzed by Kruskal wallis test. P values less than 0.05 were considered statistically significant. Results: Pro-inflammatory mesenchymal stem cells (Challenged with LPS) caused a decrease in vitality, Neutral Red-uptake and respiratory burst of activated neutrophils. Treatment of pro-inflammatory mesenchymal stem cells with higher concentration of green tea extract potentiated the decrease rate of Neutral Red-uptake and respiratory burst of activated neutrophils. Moreover, the vitality of neutrophils increased in a dose-independent manner by green tea extract.  Conclusion:It seemed that the treatment of mesenchymal stem cells in inflammatory condition with Green tea extract may potentiate the role of mesenchymal stem cells to increase phagocytosis and the respiratory burst of neutrophils cells.  
1459 Anatomy The Effect of Salvia Rhytidea Extract on the Number of Cells of Different Layers of Cerebellar Cortex Following Ischemia Reperfusion in Rats Farahmand M e Tajali M f e Department of Anatomy, Faculty of Veterinary, Shiraz University, Shiraz, Iran f Department of Anatomy, Faculty of Veterinary, Shiraz University, Shiraz, Iran 1 9 2016 21 6 536 551 09 08 2016 29 08 2016 Background & aim: Salvia has anti-oxidant oxygen free radicals which are generated during the interruption and reestablishment of ischemia reperfusion.  The aim of study was to investigate the effect of Salvia Rhytidea extract on the number of cells of different layers of cerebellar cortex following ischemia reperfusion in rats. Methods: In the present experimental study, 35 adult male rats were randomly divided into 7 groups of 5: Group 1 (control-): Sampling without ischemia. Group 2 (control +): Cerebellar ischemia with administration of normal saline. Group 3(sham): Manipulation without ischemia with normal saline administration. Group 4   received (3.2 mg/kg) aqueous and alcoholic Salvia extract 2 hours after ischemia. Group 5 received 50 mg/kg silymarin drug, 2 hours after ischemia. Group 6 received 3.2 mg/kg aqueous and alcoholic Salvia extract 72, 48, 24 and 0 h before ischemia and group 7 received silymarin drug (50 mg/kg), 0, 24, 48, and 72, hrs. before ischemia. 24 hrs. following reperfusion, the rats were euthanized and samples of the cerebellum were obtained. By using routine histological technique, the sections were stained by H&E. The measurement of cell count in cerebellar cortex were accomplished. Data were evaluated with One-Way ANOVA and Tukey diagnostic tests. Results: A significant decrease was observed in the number of neural cells in granular layer in the non-treated ischemia group and in the groups which received Salvia extract and silymarin, two hours after the ischemia (p< 0.05). No significant decrease was observed in the number of cells of this layer in the groups which received salvia extract before ischemia. But regarding the cell number of molecular and purkinje layers in above groups, no significant difference was observed compared to the control group (P˃0.05). However, no significant differences was seen in the number of cells layers compared to the control group (P˃0.05). Conclusion: Finally, administration of salvia extract and silymarin after ischemia, did not have any effect on improving the number of neuronal cells in the granular layer. However, pretreatment with Salvia rhytidea extract similar to silymarin drug improved the cerebellar injuries following ischemia. 1289 Special Investigation of Antibacterial Properties of Yeast Strains Isolated from Iranian Richal and Traditional Dairy Products in Armenia Karimpour F g Razavi SH h Nubar Tkhruni F i g Department of Nutrition, Yasuj University of Medical Sciences, Yasuj, Iran h Department of Food Science and Technology, Tehran University, Tehran, Iran, i Department of Biotechnology, Academy of Sciences of Armenia, Yerevan, Armenia 1 9 2016 21 6 552 562 30 03 2016 06 08 2016 Background & aim:The use of bio preservative or strains as sources are interesting for food bioprocessing technologist,   and is one of the latest methods to increase the shelf life of food by the health authorities . The present study aimed to investigate the antibacterial activity of supernatants of yeasts isolated from Richal as a traditional dairy product and fermented dairy products in Armenia. Methods: In the present experimental study, the purified supernatant of 77 strains of Armenian yeast products and 12 strains from Iranian Richal were isolated. The purified supernatant were tested against three strains as food spoilages bacteria includes: B. subtilis 17-89, B. Thuringensis17-89, S.typhimuium G-38 , on 3media in 2 condition as aerobic and anaerobic. The inhibition zone of the supernatant were measured   and reported as antibacterial activity. Data were analyzed using statistical tests. Result: A total of 89 strains of yeasts, three species of Rachel and 9 strains of Armenian products (13.5% percent) had demonstrated antibacterial activity. T86 strains of Armenian yeasts and FA1 (25) of Rachel had shown more ZOI and antibacterial activity on three media at both aerobic and anaerobic conditions. Comparing the mean of ZOI upon three corruption factors, Rachel strains were significantly different (p <0.05). The highest and lowest effect was observed on Bacillus subtilis effect and Salmonella typhimurium respectively. Conclusion: The results indicated that the yeast strains isolated in anaerobic and aerobic conditions on spoilage bacteria had antibacterial activity effect. Thus, it could be concluded that adding the yeast or its supernatant to food as a bio preservative, may introduce a operative product to the food industry. 1284 Sport Physiology The Effect of Aerobic Training on the Level of Growth Hormone and 17-beta Estradiol Middle-aged Women with Breast Cancer Esfahbodi A j Fathie M k Moazami M l Mohammad Rahimi GH R m j Deparement of Physical Education, Islamic Azad University, Bojnord, Iran k Depatrment of Physiology, Ferdowsi University of Mashhad, Mashhad, Iran l Depatrment of Physiology, Ferdowsi University of Mashhad, Mashhad, Iran m Depatrment of Physiology, Ferdowsi University of Mashhad, Mashhad, Iran 1 9 2016 21 6 563 575 12 03 2016 19 08 2016 Background & aim: Breast cancer is the most common type of cancer and the most common cause of death in middle-aged women in developed countries.  The results have indicated that sedentariness and hormonal changes increase the risk of breast cancer. The aim of the present study was to evaluate the effect of eight weeks of aerobic training on levels of growth hormone and 17-beta estradiol middle-aged women with breast cancer. Methods: In the present semi-experimental study, 15 women with breast cancer with the mean age of 44.46±17.15 years, weight (70.53±5.18 kg) and body mass index (27.58±2.18 kg/m2) were selected through available sampling and considered as the experimental group. The exercise program was performed three sessions per week, and every session lasting 40-60 minutes with an intensity of 30%-60% heart rate reserve (HRR) for eight weeks. Growth hormone and beta 17 estradiol levels pre and post aerobic training, 12 hrs of fasting and with 24 hrs of severe Lack of physical activity was measured during of eight weeks. Data were analyzed by paired samples t-test at a significance levels of P<0.05. Results: The growth hormone levels did not change significantly after eight weeks of aerobic exercise (P=0.447), whereas 17-beta estradiol levels decreased significantly (P=0.000). Conclusion: It seemed that eight weeks of aerobic exercise could reduce one of the breast cancer markers (17-beta estradiol); so these training are recommended for the improving or prevention of breast cancer. 1363 Psychology The Effectiveness of Mindfulness-based Parenting Training on Anxiety, Depression and Aggression of Mothers with Children Suffering from Oppositional Defiant Disorder Moradiyani Gizeh Rod SK n Mir Drikvand FA o Hosseini Ramaghani N p Mehrabi M n Department of Psychology, University of Lorestan, Lorestan, Iran, o Department of Psychology, University of Lorestan, Lorestan, Iran, p Department of Psychology, University of Lorestan, Lorestan, Iran, Department of Educational Sciences, Islamic Azad University, Hamadan, Iran 1 9 2016 21 6 576 590 06 06 2016 29 08 2016 Background & aim: Mothers with children suffering from Oppositional Defiant Disorder experience greater problems related to mental health including anxiety, depression and aggression. In this regard, the aim of the present study was to evaluate the effects of a mindfulness-based parenting training in anxiety, depression and aggression of mothers with children suffering from Oppositional Defiant Disorder.  Methods: The present semi-experimental study consisted of a pretest-posttest control group. The statistical population of the study included all mothers who had a female student with Oppositional defiant disorder (age group 6 to 12 years) of primary school in Nour Abad, Iran. Sampling was conducted in two stages, random and purposive. For this purpose, 34 mothers of girls with Oppositional defiant disorder after purposive sampling were randomly assigned to experimental and control groups (17 in each of groups). The Child Behavioral Checklist (CBCL) and Teacher Report Form (TRF) with clinical interviews were used for screening. Also, the anxiety and depression inventory and aggression questionnaire were used to collect data in two conditions (pretest and posttest). Mindful parenting course was implemented for the experimental group in 8 sessions, 2 hours each. Data were analyzed using SPSS software (ver. 23), descriptive statistical parameters (mean and SD) and univariate analysis of covariance (ANCOVA). Results: Results of ANCOVA indicated that the Mindful Parenting course directed to a statistically significant difference between pre-and post-test scores which led to decreasing scores of anxiety, depression and aggression. So that in the Post-stage, after controlling for pre-test scores, the experimental group reported less anxiety, depression and aggression compared to the control group. Conclusion: With regard to the effective use of Mindful Parenting course to decreasing symptom-related with anxiety, depression and aggression in mothers of girls with Oppositional defiant disorder, the training program should be directed toward reducing stress and promotion of general health in this group of people.   1332 Microbiology The Study of Pantone Valentin Leukocidin (PVL) Gene in Methicillin-resistant Staphylococcus Aureus Isolated from Blood and Wound in Zahedan, Iran Tahmasebi H Bokaeian M Department of Microbiology, Zahedan University of Medical Science, Zahedan, Iran Department of Microbiology, Zahedan University of Medical Science, Zahedan, Iran 1 9 2016 21 6 591 604 30 04 2016 29 08 2016 Background & aim: Panton-Valentine leukocidin (PVL) is a Staphylococcus aureus gamma toxin. There may be a link between Meticillin-Resistant Staphylococcus aureus and Panton-Valentine leukocidin (PVL), as an important cytotoxin found particularly in severe infections. The purpose of this study was to isolate and identify Staphylococcus aureus virulence Panton-Valentine leukocidin (PVL) and methicillin resistance genes in clinical samples using PCR techniques. Methods: In the present cross-sectional study, 89 isolates were collected from blood samples and detected as Staphylococcus aureus during the period of 6 months at Ali Ebne AbiTaleb Hospital, Zahedan, Iran. Initially, the case study examples were examined by biochemical tests. Then, based on recognized standards, Staphylococcus aureus isolates were isolated. Afterwards, isolates obtained were confirm by using 16srRNA gene. Subsequently, the antibiotic susceptibility of all isolates to methicillin was determined using Cefoxitin(30µg) disk diffusion and agar screening methods. Finally, the PCR method was used to determine PVL and mecA genes. All results were analyzed by the Chi-square test. Results: Out of the total 89 isolates of Staphylococcus aureus blood isolates, 26 isolates from wounds and 63 were isolated. Most samples were obtained from female patients. According to the molecular analysis, 47 isolates (82/52%) were mecA gene and resistant to methicillin and 42 strains (47/2%) was methicillin-sensitive. Resistance to vancomycin wasn’t observed in isolates. Erythromycin and Penicillin had the highest prevalence of antibiotic resistance among isolates, respectively. Multi-resistant strains were the most PLV genes frequent. A significant relationship was observed between the resistance and the presence of Pantone Valentin (P≥0 / 05). Conclusions: Due to the frequency of mecA and PLV genes in resistant and susceptible strains of Staphylococcus aureus, and also the distribution of Pantone Valentin gene in resistant strains; therefore, based on the statistical analysis, we can conclude that there may be a connection between these two factors (P≥0 / 05). 1227 Microbiology Determination of Phylogenetic Relationship Among Vibrio Parahaemolyticus Isolates from Persian Gulf and the Evaluation of their Susceptibility to Antibiotics Haghnegahdar S Baserisalehi M Department of Microbiology, Shiraz Branch, Islamic Azad University, Shiraz, Iran, Department of Microbiology, Kazeroun Branch, Islamic Azad University Kazeroon, Iran 1 9 2016 21 6 605 616 19 01 2016 29 08 2016 Background & aim: Vibrio parahaemolyticus is a Gram negative halophilic bacterium found in aquatic environments. This bacterium has been introduced as a cause of acute gastroenteritis following the consumption of raw and uncooked seafood. Major symptoms of Vibrio parahaemolyticus illness is watery diarrhea, abdominal cramps, Nausea, Vomiting, Fever, Headache and Bloody diarrhea. The purpose of this study was isolation and identification of Vibrio parahaemolyticus from Persian Gulf and the determination of their phylogenetic relationship. In addition, antibiotic susceptibility of the isolates was evaluated in order to achieve maximum information concerning a drug of choice. Methods: In this study, 89 samples of water, sediments, fish and shrimp were collected from different regions of the Persian Gulf. All samples were asssessed for phenotypic and molecular isolation and identification of Vibrio parahaemolyticus. Then the positive and negative  strains of conagua vibrio parahaemolyticus were determined based on hemolysin production. the identification and phylogenetic relationship  were  analyzed using the mega-6 software. Finally susceptibility of Kanagawa positive Vibrio parahaemolyticus to antibiotics was evaluated by disk diffusion method and minimal inhibitory concentrations of effective antibiotics were assessed using double serial dilution method. Results: A total of nine Vibrio parahaemolyticus were isolated and identified. Of all isolates five strains were Kanagawa positive and four were Kanagawa negative. All isolates exhibited phylogenetic relationship to each other except one strain (NSP1). The results obtained from antibiotic susceptibility of Kanagawa positive  Vibrio parahaemolyticus isolates illustrated that most of the isolates were resistance to Vancomycin, Oxacillin, and Amikacin and susceptible to Trimetoprim Sulfometaxazole respectively. In addition, the lowest Minimal inhibitory Concentration (MIC) value was found for Sulfometaxazole  and highest MIC value was found for Nitofuran. Conclusion: The present study illustrated that both pathogenic and nonpathogenic Vibrio parahaemolyticus existed in the persian Gulf. On the other hand these bacteria that could facilitate phylogenetic relationship with each other because of  horizontal gene transfer among them.Trimetoprim Sulfometaxazole could be considered a durg of choice for treatment of the patient suffering from Vibrio parahaemolyticus infections.   1228 Psychology Comparing the Performance of Five Brain Systems and mood changes (Anxiety and Depression) with the Level of Estrogen in Postmenopausal and Premenopausal Asgari K Hashemi B Department of Psychology, Faculty of Humanity, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran, Department of Psychology, University of Isfahan, Isfahan, Iran 1 9 2016 21 6 617 629 19 01 2016 19 08 2016 Background & aim: One of the most critical stages of women’s lives is menopause. It is caused by permanent cessation of ovarian function and decreased of estrogen level. Menopause has numerous side effects (Physical and psychological). Recent neurophysiological data suggest that the function of central nervous system may be susceptible to modulation by estrogen. The aim of this study was to compare the performance of the five important parts (prefrontal cortex, cingulate gyrus, limbic system, basal ganglia and temporal cortex) of the brain and mood changes (anxiety and depression) with the level of estrogen in postmenopausal and premenopausal women in Isfahan. Methods: In the present descriptive study 200 women (100 postmenopausal women 45 to 65 years old and 100 women, premenopausal 20 to 40 years old) were selected by sequentially available from health centers of Motahari, Sajjad and Amin Askariyeh Hospital. The research instrument was a brain systems questionnaire of 101 questions (Brain systems) with 89/0 reliability to evaluate five important system functions in the brain (prefrontal cortex, cingulate gyrus, limbic system, basal ganglia and temporal lobe) and Beck Depression Inventory with reliability of 93 / 0 and anxiety with reliability 92/0 to assess depression and anxiety. The data was analyzed using descriptive statistics (mean and standard deviation) and inferential statistics (MANOVA MANOVA) analysis. Results: significant differences were found in five brain system functions (prefrontal cortex, cingulate gyrus, limbic system, basal ganglia and temporal cortex) and levels of estrogen and anxiety of two the groups of postmenopausal and premenopausal women (P<0.001) but no significant difference was observed regarding depression of these groups (P>0/001).         Conclusion: It seems that in addition to the role of estrogen in the female reproductive system, it also has an impact on the nervous system and its various parts. Following the reduction of estrogen in postmenopausal women, the modulation of brain functions is affected. So the two-way interaction and convergence among secretion of hormones with brain function and mood changes such as anxiety and depression could be explained.