1 1728-6506 Yasuj University Of Medical Sciences 1110 General The effect of two weeks morning and afternoon special training program on performance and plasma levels of IL-17 and cortisol in wushu athletes ghayeblo B b ghasemnian AA c Azad A d b Department of Physical Education and Sport Sciences, University of Zanjan, Zanjan , Iran c Department of Physical Education and Sport Sciences, University of Zanjan, Zanjan , Iran d Department of Physical Education and Sport Sciences, University of Zanjan, Zanjan , Iran 1 2 2016 20 11 947 961 29 10 2015 03 01 2016 Background & Aims: Recent evidences (knowing the effects of time on physiological variables) show that the human body undergoes a lot of changes during the day and the night and has a special ability in each hour. Competition in the morning Confronts athletes with the problem of susceptibility to infection and inflammation. The purpose of this study was to examine The effect of two weeks morning and afternoon special training program on performance and plasma levels of IL-17 and cortisol in boy wushu athletes Materials and Methods: Fourteen sanda boy athletes were purposefully selected and after Bruce and  rast tests run, divided into the two Similar groups morning-time training group (N = 7, training time 9 a.m.) and evening-time training group (N=7, training time 18 pm ). The Subjects were trained for two weeks (6 session in each week) according to an Sanda sport specific training program. Values of peak anaerobic power, average anaerobic power, minimum anaerobic power and fatigue index measured with Field  rast test. To determine the amount of plasma IL-17 and cortisol , the participants’ blood samples was taken In the fasting state three times, 24 h before the first training session in baseline status and Immediately after tests at 9 am and 48 h after the last training session immediately after tests performance at 9 a.m. Data were analyzed using repeated measures, independent-samplesT-test, ,paired- samplesT-test  and Covariance. Results: The results showed that there were significant differences between the amounts of plasma IL-17 of the two groups after two weeks(P<0.05). But there were not any significant differences between the amounts of plasma cortisol and performance of both groups after two weeks(P>0.05). Conclusion: It seems likely transition training time to the morning, 2 weeks before the competition in the morning, decrease inflammatory factors such as IL-17 in sanda boys. Therefore appears that two weeks transition training time from evening to morning can increase the safety of athletes immune during the competition.
1114 Nursery- Psychology Effectiveness of Eye Movement Desensitization and Reprocessing on Anxiety in Children with Thalassemia in a 12-month follow up Shahnavazi A e behnammoghadam M f Abdi N g Rigi kh h Allahyari E i Bakhshi F j Peighan A k behnammoghadam A l e 1Department of Nursing, Nursing and Midwifery School, Iranshahr University of Medial Scieneces, Iranshahr, Iran. f Department of Critical Care Group, Yasuj University of Medical Sciences, Yasuj, Iran g Department of Critical Care Group, Yasuj University of Medical Sciences, Yasuj, Iran h 3Department of Midwifery, Nursing and Midwifery School, Iranshahr University of Medial Scieneces, Iranshahr , Iran. i Department of Nursing, Nursing and Midwifery School, Iranshahr University of Medial Scieneces, Iranshahr, Iran. j Student Research Committee, Yasuj University of Medical Sciences. Yasuj, Iran k 1Department of Nursing, Nursing and Midwifery School, Iranshahr University of Medial Scieneces, Iranshahr, Iran. l 7 Department of Psychology, Islamic Azad university, Science and Research Branch yasuj, Yasuj, Iran 1 2 2016 20 11 962 971 31 10 2015 15 01 2016 Background and  Objective: Thalassemia is the most common genetic diseases in the world and especially in our country. For many reasons, including chronic disease, health care costs, the expected mortality Ray disease states such as anxiety, psychological and social problems in thalassemia. The aim of the present study was to assess the efficacy of Eye Movement Desensitization and Reprocessing (EMDR) on anxiety of children with thalassemia in a 12-month follow up..  Methods: It was a clinical trial study, which was performed on 60 patients with Thalassemia in 2013-2014. A total of 60 thalassemic patients based on Beck anxiety inventory, selected using sampling and were randomly divided into experimental and Control groups. in experimental group, eye movement desensitization and reprocessing therapy was perfomed in five sessions and The control group received no intervention. In order to collect information on demographic questionnaire and Beck Anxiety Inventory were used. data were gathered on anxiety symptoms at pretreatment, posttreatment and 12 month follow up and were analyzed using descriptive statistics, repeated measures and chi square. Results: The average age of the participants was 15.22±1.93  years old. This study shows that the mean or average level of anxiety children with thalassemia in the experimental group before and after intervention and in a 12-month follow up group 34.73 ± 5.62, 19.90 ± 2.48 and 19.23 ± 2.48 respectively; repeated measures ANOVA showed significant statistical difference (p<0.001). Mean  of anxiety symptoms in before , after intervention and 12 month follow up in control group was 34.93± 5.69 , 34.53± 5.61 and 33.86±5.83 respectively, which using with  repeated measures ANOVA did not show significant difference (p>.05) Conclusion: This study shows that Method of eye movement desensitization and reprocessing as an alternative method to treat or reduce anxiety, aggressive children with thalassemia .Trained Nurses can use this new method is effective in the treatment of anxiety. 1007 Physiology Study of antinociceptive effects of Pelargonium geraveolens L. leaves hydroethanolic extract in male mice Heydari N m Mirazi N n m Islamic Azad University, Hamedan, Iran n Bu-Ali Sina University, Hamedan- Iran. 1 2 2016 20 11 972 984 12 08 2015 17 12 2015 Running title: Anti nociceptive, Pelargonium graveolens Abstract: Introduction: Pain is a somatosensory sense that as an indicator to diagnoses for diseases . Pelargonium graveolens is known as a medicinal plant with use in traditional medicine. The aim of this study is to evaluate the analgesic effect of Pelargonium graveolens leaves extract (PGE) in male mice Material and Methods: In this experimental study, 42 male mice were divided in 6 groups (n=7). The control group(taking normal saline,0.25 ml, i.p), morphine group( 1mg/kg, i.p), treated groups with PGE at doses of 100, 200 and 600mg/kg and group induced with naloxone (0.1mg/kg, i.p) + 200mg/kg of PGE. In order to evaluate the analgesic effects of PGE the tail flick and writhing tests were used. The data for each test were compared with One-way ANOVA and Tukey's post test. Results: Our results showed that PGE (200 and 600 mg/kg) increased pain thershold compared with control group in writhing and tail flick test significantly (P<0.001). Conclusion: The PGE has anitinociceptive effects in male mice. This analgesic effect of Pelargonium graveolens extract probably related to its flavonoids composition which has effect on opioid system. 974 Immunology Effect of hydroalcholic extract of Cynodon dactylon the phagocytosis and respiratory burst of peritoneal macrophages of NMRI mice Abtahi Froushani SM o o Urmia auniversity 1 2 2016 20 11 985 995 22 07 2015 01 01 2016 Background: The anti-inflammatory effects of Cynodon dactylon have been determined in some previous studies. Nevertheless, there is no comprehensive study about immunomodulatory effects of C. dactylon. The present study was performed to investigate the modulatory effects of C. dactylonon macrophages functions of NMRI mice. Methods: This survey was an experimental intervention study. The  study  population  consisted  of  14  male  NMRI  mice  randomly  categorized  into  3 treatment groups and one control group (each group was contained 10 mice). Mice in treatment groups received hydroalcoholic extract of C. dactylon for 3 constitutive weeksin different doses (100,200 and 400 mg/Kg- every day, orally). Control mice received PBS at the same volume. At the end of study, macrophages isolated from peritoneal cavity of mice and the neutral red uptake, respiratory burst after challenge with tetra phorbol acetate and respiratory burst after challenge with opsonized yeast were evaluated in these population. Data were analyzed using the one-way ANOVA plus Turkey’s test. P values of less than 0.05 were considered statistically significant. Results: The results of stimulation of macrophages with ester of phorbol showed a significant increase in respiratory burst of macrophages isolated from treatment groups compared to control mice (p<0.01). Nevertheless, when macrophages challenged with opsonized yeast, there was no significant difference between groups .Moreover, the results of neutral red uptake by macrophages didn’t show any significant difference between macrophages of control and treatment groups. Conclusion: However, the hydroalcoholic extract of C. dactylon caused a significant decrease in the production of the potentially harmful free radical, but it could not interfere with the function of macrophages after challenge with a real infection. 980 General Effect of hydro-alcoholic extract of Carum copticum on withdrawal syndrome in adult rats addicted to morphine honarvar A p delaviz H Jafari Barmak M Roozbehi A p Yasuj Univesity of MedicalSciences Yasuj Univesity of MedicalSciences Yasuj Univesity of MedicalSciences Yasuj Univesity of MedicalSciences 1 2 2016 20 11 996 1010 26 07 2015 14 12 2015 Background & Aim: Long-term useofopioidcreates toleranceandphysical and psychologicaldependence. Discontinuation of the drug, creating ashortageofendogenousopioidsandwithdrawal syndromeemerges.Addictiontreatmentin traditional medicine isusing ofherbssuch asCarumcopticumthathas manytherapeutic effects. The purpose of thisstudy was to evaluatethe effects of hydro-alcoholic extractof Carumcopticum onwithdrawalsyndromein adult ratsaddictedto morphine. Material & Method: In this study70male Wistarratsdividedinto seven groups of 10, six groups were addicted by morphinesulfateinjection (the first 5days10 mg/kg, the second 5days15 mg/kg andon the dayfrom11 to21, 20mg/kg) subcutaneouslyfor 21 days.Groups three, four and five were fed respectively 10%, 20% Carumcopticum extract and methadone (mg/kg 5) after addiction of animals until the end of period. Groups sixandseven were given respectively10%and 20% of Carumcopticumextractfrom the beginning to the end of treatment period orally.Group one(control) andGrouptwo (morphine) were fed daily 0.5 mL of saline orally to the end of the treatment period (day 21to 35).Datacollected from body weight, jumping, itchingand diarrhea ofanimals wereanalyzedby SPSS software, using Unpaired T test, ANOVA and LSD as post-hoc statistical tests. Finding: Comparing the mean of weight loose ofthe animals in different treatmentgroupson day21with 35showed statistical significant reduction in the group that received extract of Carumcopticum 10%. On the day 21,itchingand jumping, in the group that received extract of Carumcopticum 10%,and the diarrhea, in the group that receivedthe extract ofCarumcopticum20%compared withthe control groupshoweda significant decrease(P<0.05).On day35,Jumping and diarrheain thegroupsreceived theextract ofCarumcopticum10 and 20percent comparedwith the control groupshoweda significant decrease(P<0.05). Conclusion: According to the results, hydro-alcoholic extract of carumcopticum inconcentration of 10and 20percent are effectivein reducingwithdrawal syndromeofmorphine as like as methadone.Perhapsitcan be usedas an alternative tomethadoneinaddictiontoreducepainfulwithdrawalsyndromeand freed from the side effects ofmethadone.This suggestionneeds a widerinvestigation. 913 Special Investigation of Interleukin-17 gene polymorphism on DAP-Kinase gene promoter methylation in Patients with Breast Cancer Naeimi S Islamic Azad university,kazeron,iran 1 2 2016 20 11 1011 1023 23 06 2015 15 01 2016 Background & Aim: Breast cancer is the most common malignancy in women . Studies have shown that increased in methylation of CpG islands (CpG island hyper methylation, CIHM), is one of the important mechanisms in gene down regulation. DAP-Kinase protein plays an important role in the process of Apoptosis. Interleukin-17 is an proinflammatory cytokine and inflammation,is one of the factors  that affect on gene methylation . the purpose of this study was to evaluate the polymorphism of the IL-17 gene promoter methylation Dap-kinase and its relationship to breast cancer. Methods: In this case - control study, A total of 40 Women with Breast cancer and 40 healthy women in Iran were examined.DNA was extracted by saluting out method and Single nucleotide Polymorphisms of the IL-17 gene were analyzed by the PCR-RFLP method and To study gene promoter methylation Dap-kinase, MSPCR method was used.data were compared in both groups by using Pearson’s chi-square and Hardy-weinberg equilibrium test. RESULTS: Results confirm the fact that, there is a relationship between DAP-kinase gene promoter methylation and breast cancer disease So that the promoter of this gene in patients than in healthy individuals was much more methylated( p<0.05) . On the other hand there is no significant coloration between IL-17 gene polymorphisms and DAP-kinase gene methylation (P>0.05) Conclusion: Due to the fact, that promoter genes methylation is one of the mechanisms of epigenetic genes silencing, it seems that DAP-kinase gene promoter methylation increases is associated with the risk of breast cancer in women. 1132 Special Association of CYP17 and SRD5A2 gene polymorphisms with Prostate cancer risk among Iranian and Indian populations onsory kh Mousavi M Jalilvand E Islamic azad university Biology Department, Sistan-Bloochestan University, Zahedan Biology Department, Faculty of Science, Damghan University, Damghan, 1 2 2016 20 11 1024 1035 16 11 2015 20 01 2016 Aims and objectives: Prostate cancer is a complicated disease that genetics and environmental factors may be playing a promoting role in its progression. Polymorphism of genes such as steroid hormone receptors are having very important role in developing this disease. One such gene, CYP17 is playing role in hydroxylation and SRD5A2 gene, the predominant 5&alpha-reductase isozyme in prostate, catalyzes the conversion of testosterone into the more potent androgen, dihydrotestosterone (DHT), which is required for the normal growth and development of the prostate gland. The purpose of this study was to investigate association of CYP17 and SRD5A2 genes polymorphisms with prostate cancer risk. Materials and methods: PCR-RFLP analysis of CYP17 and SRD5A2 genes were performed on 100 prostate cancer patients admitted to the Department of Urology, Postgraduate Institute of Medical Science and Research (PGIMER), Chandigarh, India, and 150 patients from Imam Khomeini Hospital, Tehran, Iran, compared with equal number of matching controls for each group visiting same centers for other reason. The data was analyzed using the computer software SPSS for windows (version 19), using logistic regression. Results: In this case-control study, there was a significant increase with risk of prostate cancer association for individuals carrying one copy of CYP17 A2 allele in Iranian (OR= 2.10 95% CI, 1.03-4.27 P=0.041) and Indian populations (OR= 2.16 95% CI, 1.08-4.33 P=0.029). While the risk was decreased in individuals having two A2 alleles in both groups. Compared with men having the VV genotype of SRD5A2 gene, there was no significant association between the VL genotype and the risk of prostate cancer among Iranian (OR, 0.87 95% CI, 0.49 -1.56 P=0.661) and Indian (OR, 0.99 95% CI, 0.54 -1.81 P=0.989) patients. Also there was no difference in the occurrence of the genotype LL between prostate cancer patients and control groups in both studied populations therefore, there was no association between this genotype and prostate cancer risk. Conclusion: It seems to be an association between A2A2 genotype of CYP17 gene with the risk of prostate cancer but no association was found with any alleles of SRD5A2 gene with this risk. 917 Epidemiology Spatial distribution of cancer in Kohgilooyeh and Boyerahmad province Fararouei M Aliakbarpoor M Tabatabai HR Parisai Z Mari Oriad H Jamshidi A Department of Epidemiology, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran Department of Health, Shiraz University of Medical Sciences, Shiraz, Iran, Department of Epidemiology, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran, ُDepartment of Health, Yasuj University of Medical Sciences, Yasuj, Iran. ُDepartment of Health, Yasuj University of Medical Sciences, Yasuj, Iran. Social Determinants of Health Research Center, Yasuj University of Medical Sciences, Yasuj, Iran. 1 2 2016 20 11 1036 1048 26 06 2015 16 01 2016 Spatial distribution of cancer is one of the powerful tools in epidemiology of cancer. The present study is designed to understand the geographical distribution of most frequent types of cancer in K&B province. Methods: All registered cases of cancer are reviewed and duplicate cases were removed. The data was analyzed using Arcgis software. Results: Of all registered cases, 1273  remained for analysis of which 57% were residences of urban areas. Cities including  Sisakht, Yasuj and Dehdsasht were shown to have highest incidence rates among the Urban areas. Dena, Sepidar and Kohmare Khaleghi had the highest rates among the rural areas in the province. Skin cancer was the most common type of cancer which had the highest rates of incidence in Sisakht and Dehdasht and Dena and Sepidar among urban and rural areas respectively. Conclusion: The distribution of cancer was not even in the province. Attitude and consumption of wild and regional plants are introduced as the potential risk factors for such a spatial distribution of the common cancers I the province. The results of this study could be used for further analytical studies to understand the regional etiology of cancer in the province.