1 1728-6506 Yasuj University Of Medical Sciences 204 Special The Effect of Morphine Dependency on Serum Levels of Trace Elements (Copper, Zinc, and Manganese) in Male Rats Yousofvand N Ghowsi M Pourmotabbed A 1 6 2014 19 3 195 203 13 04 2015 13 04 2015 Background & aim: In some studies, changes in trace elements of abuse of addictive substances have been mentioned. Due to the effects of trace elements on the immune system and their effects on susceptibility to infectious agents, the purpose of this study was to investigate the effect(s) of morphine dependency on the concentration of trace elements (copper, zinc and manganese) levels in serum of rats. Methods: In the present study, twenty-four adult male Wistar rats were randomly divided into two groups of receiving morphine (experimental) and control one. In the experimental group, morphine were injected subcutaneously twice daily for thirty days. Morphine dose on the first day was 10 mg/ml/kg daily and increased 2 mg/kg daily until it reached a maximum of 68 mg/kg. Instead of morphine, the control group received 1 ml/kg of normal saline for 30 days. Withdrawal syndrome in morphine-dependent groups and the control group were injected with 4 mg/kg naloxone in four animals from each group and then evaluated. Serum concentrations of zinc, copper and manganese were determined by spectrometer coupled plasma optical emission detector. Data were analyzed by Student's t test. Results: Serum concentrations of manganese in morphine-dependent group compared with the control group was significantly lower (p<0.05), whereas serum zinc and copper concentrations were significantly higher than the control group (p<0.05). Results showed a significant increase in serum copper and zinc concentrations and decrease in serum manganese level in morphine dependent rats. Conclusion: Dependence to morphine may be affecting the status of trace elements in serum of male rats.
205 Special Evaluation of gene rs2623047 polymorphism of human sulfatase1 in women with recurrent abortion compared with women with normal fertility Taghizadeh E Bakhshiganjae M Taghizadeh H Baniamerian F 1 6 2014 19 3 204 211 13 04 2015 13 04 2015 Background & aim: recurrent miscarriage is defined as accruing of at least 3 clinical spontaneous abortions or more. Several factors including genetic and environmental factors are involved in it. Among them, sulftase1 gene rs2623047 has a role in the human embryonic development. The purpose of this study was to evaluate sulftase1 gene rs2623047 polymorphism in women with recurrent abortions referred to the infertility center of Yazd, Iran. Methods: In the present case-control study, sixty-five healthy women were selected as controls and thirty-five women with recurrent miscarriage were included as cases. Blood samples were taken from both groups manually, using a low-salt DNA extraction and amplified fragment polymorphism rs2623047 1 sulfatase gene made by PCR and enzymic digestion conducted using BstNI enzyme. Results were analyzed using the chi-square test. Results: GG genotype in cases were 42±9% and in control group 5/18% respectively (p=0.003). While the AG genotype in the control group was 9.56% and in the cases 1.37% (p=0.003). Conclusions: Genetic variation in sulfatase 1 gene may have a role in embryonic development during pregnancy and larger studies with more samples along with other polymorphisms in this gene may be helpful. 206 Special Kargar M Gholami M Doosti A Najafi A Aeein V 1 6 2014 19 3 212 222 13 04 2015 13 04 2015 207 Special Investigating Awareness Amount of Nursing Students of Medical Sciences University of Bushehr about Ethic in Nursing Profession -2013 Jahanpour F Khalili A Ravanipour M Nourouzi L Khalili M Dehghan F 1 6 2014 19 3 223 232 13 04 2015 13 04 2015 Background & aim: Nurses' ethical responsibility in practice and care is required to be aware of the principles of professional ethics. The aim of this study was to determine nursing students' knowledge of ethics in nursing of Bushehr University of Medical Sciences. Methods: In the present analytical-descriptive sectional study, in which the participants are 4-8 semester nursing students of Bushehr University of Medical Sciences. The research tools for collecting information were tow-section questionnaires consisting of demographic data and specialized questions about ethic and rules in the nursing profession. Data analysis was performed using the SPSS software by using independent t-tests and chi-square. Results: Total awareness of 4-8 semester nursing students about ethic and rules in nursing profession was intermediate (53.78 percent). There was a considerable relation between sexuality and satisfaction (p.436). A considerable relation between students' educational semester and satisfaction amount was not also not observed (p>.927). Conclusions: Students' awareness about professional ethic wasn't very desirable so it is suggested that by holding moral workshops in nursing or settling moral courses in nursing students curriculum will increase the amount of nursing students' awareness about nursing ethics. 208 Special Molecular Analysis of Gene Frequencies of TEM, CTX-M and SHV in Beta-Lactam Antibiotic-Resistant Strains of E. Coli Isolated from Urinary Tract Infections in Yasuj Hospitals Mortezavi R Khosravani SAM Naghavi NS 1 6 2014 19 3 233 241 13 04 2015 13 04 2015 Background & aim: Urinary tract infections is one of the most common infectious diseases which many factors are involved, but bacteria such as E.coli is the most important agent of urinary tract infections. Antibiotic resistance as a major problem in the treatment and control of these infections is considered. The aim of this study was to determine the genes that cause resistance to beta-lactam family of antibiotics on E.coli isolated from urinary tract infections in Yasuj city. Methods: In the present Cross-sectional study which was conducted over a period of seven months in 2013, 123 samples of E.coli were collected from Yasuj hospitals for molecular analysis of TEM, SHV CTX-M genes, causing antibiotic resistance by (PCR) method.Data were analyzed using SPSS statistical test. Results: PCR showed that the gene frequency of TEM (50.94%), SHV (47.16%), CTX-M-9 (35.84%), and CTX-M-10, (32.07%) and the highest and lowest prevalent of genes were related to TEM and CTX-M10 in E.coli isolated from urinary tract infections respectively. Conclusion: According to the high prevalence of resistance to beta-lactam antibiotics, the current study showed that the noted genes play an important role in facilitating the spread of antimicrobial resistance in this region. 209 Special Impact of Oral Contraceptives on the Quality of Life of Women Referred to Health Centers of Yasuj, Iran Goodarzi F Hossieni M Moomeni E 1 6 2014 19 3 242 251 13 04 2015 13 04 2015 Background & aim: Oral contraceptives are one of the most commonly and effective reversible contraceptive methods. This method has benefits and side effects that should be considered as a factor affecting the patients’ quality of life. The aim of this study was to determine the effect(s) of oral contraceptives on the quality of life of the consumers. Methods: In the present case-control study which was conducted from January 2011 to June 2012, the quality of life of 105 patients who were first time users of oral contraceptive pills were selected and compared with 100 of those that had used traditional methods or did not use of any methods referred to The Yasuj Health Center. Before and after three months of using, the sf36 questionnaire obtaining the quality of life in women was investigated. Data were analyzed using the paired t-test and chi-square test. Results: Quality of life domains in consumers of oral contraceptive pills after three months showed no significant difference (P>0.05). Domain scores for physical limitation of oral contraceptive consumers were significantly reduced (p=0.01). Conclusion: There is no negative impact on quality of life of oral contraceptive consumers. In addition, no significant differences after three months of consuming such pills were observed in the consumers’ life domains. 210 Special Determination the Amount of Illegal Furazolidone Residues in Broilers in Ahvaz Abattoir by HPLC Method Fazlara A Mayahi M Najafzadeh Varzi H Gudarznia F Mohammadyari S 1 6 2014 19 3 252 264 13 04 2015 13 04 2015 Background & aim: due to the risks to human health, particularly in terms of carcinogenicity, the application of Furazolidone in animals which their products are consumed by human population is strongly prohibited. The purpose of this study was to determine the amount of unauthorized furazolidone in broiler chickens slaughtered in Ahvaz by using HPLC. Methods: In the present cross-sectional study which was conducted within six months, 100 broiler carcasses were randomly collected from Ahvaz slaughterhouses. Then by using ice, the carcasses were transported to the laboratory in less than 24 hours inorder to illicit the amount of furazolidone drug by the HPLC method. After extraction and degrading processes, using Ethyl-acetate and related protocols for isolation of Furazolidone from muscles, and also calibrating HPLC system to obtain standard curves, the amount of 20 microliters of each sample was injected to the HPLC device and the amounts of Furazolidone were determined in the mixture of pectoral and femoral muscles. Finally the obtained results were statistically analysed by using one sample t-test in the SPSS software. Results: The mean Furazolidone concentration in the mixture of femoral and thorasic muscles was 28.15±2.37 mg/kg. Thirty-nine percent of the samples were positive for containing illegal Furazolidone residue. Conclusion: According to the results of the current study, more attention is seriously recommended by authorized responsibilities to prevent the antibiotic residues such as furazolidone in poultry meat. 211 Special Predict Resiliency Based On the Rate Of Emotional Intelligence In Public Organizations In Yasuj Karimi Z Mohammadi K Zarei E Zadehbagheri GH 1 6 2014 19 3 265 274 13 04 2015 13 04 2015 Background & aim: resiliency is the human ability in dealing with high-risk and traumatic conditions. Nevertheless, not all individuals are equally abiding, and resiliency can be increased or decreased by various factors. The aim of this study was to investigate the effect of emotional intelligence in the level of resiliency among employees in public organizations of Yasuj, Iran. Methods: In the current correlational study, 382 state employees of Yasuj (221 males and 161 females) were selected using multistage cluster sampling. For variable measurement, the Emotional Intelligence Scale and Connor-Davidson resilience scale were used. The collected data were analyzed by the correlation coefficient and regression methods. Results: Total score of emotional intelligence with resiliency revealed a significant positive relationship. Emotional intelligence can predict some degree of resilience. Emotional intelligence and resiliency among men is more than women. Conclusion: If the level of emotional intelligence goes higher, the possibility of employee’s resiliency in risky circumstances also increases. 213 Special The Role of Guide Wire in Internal Urethratomy Under Direct Vision Rabani SMR Niknam SH 1 6 2014 19 3 275 282 13 04 2015 13 04 2015 Background & aim: Urethral Stricture (US) is a common urolologic problem. Direct optic internal urethratomy (DOIU) is a common option for treating US. The purpose of this study was to investigate the role of the guide wire to facilitate direct vision internal in adult male patients. Methods: In this prospective study, 84 patients with anterior urethral stricture randomly divided into two groups. The first group underwent routine DOIU surgery and the second group internal urethratomy was carried out using Wolf ureteroscope 8-9.8 French and guide wire 0.035 French which inserted into bladder. Data were collected and analyzed using software (spss). Results: The mean operation time was 14 minutes in group one and for group 2 was 10 minutes. The comparison of complications after surgery showed that in the first group urethral bleeding accrued in 4 patients (9.52%) and fever infection was seen in 3 patients (7.14%) respectively but no such events were observed in group 2. The only late complication in both groups was recurrence of the stricture that was seen in 8 patients (19.04%) of group one and 3 patients (7.14%) of group 2. Conclusions: The results of this study showed that using a guide wire during DOIU may facilitates the procedure shortens the operation time and reduce the effects of short and long-term operation.