1 1728-6506 Yasuj University Of Medical Sciences 3137 Physiology The Relation between Intracellular Calcium Concentration and Reactive Oxygen Species Production in Fresh and Cryopreserved-Thawed Human Spermatozoa Iravanpour F b Keshtgar S c Ebrahimi B d b Shiraz Neuroscience Research Center, Shiraz University of Medical Sciences, Shiraz, Iran c Department of Physiology, School of medicine, Shiraz University of Medical Sciences, Shiraz, Iran d Shiraz Geriatric Research Center, Shiraz University of Medical Sciences, Shiraz, Iran 1 11 2021 26 5 729 743 04 08 2021 23 09 2021   Background & aim: Storing frozen sperm in liquid nitrogen is a widely used method of preserving them for a long time. Many of these sperms are not damaged or survive after thawing, and many lose their motility. These injuries are caused by damage to the sperm membrane and changes in permeability to ions due to changes in osmotic pressure, the formation of ice crystals, and increased production of oxygen free radicals. The aim of the present study was to determine the relationship between intracellular calcium concentration and the production of oxygen free radicals in fresh and frozen human-thawed spermatozoa.   Methods: In this quasi-experimental study conducted in 2013, 35 samples of fertile human cement were randomly selected and divided into two groups: fresh and frozen-thawed. The sperm of the frozen-thawed group were preserved under liquid nitrogen for one month. The effect of 10 µM calcium ionophore A23187 was evaluated on fresh and thawed sperm motility, viability, and acrosome reaction. Oxygen free radical production and intracellular calcium content were evaluated using luminol and Flou-3/AM staining by chemiluminescence and flowcytometric method, respectively. Data were compared using the Mann–Whitney test. P<0.05 was considered statistically significant.   Results: The percentage of alive, motile and progressive sperm and the percentage of sperm with intact acrosome decreased significantly after the freezing-thawing process. The addition of A23187 to the medium increased intracellular calcium in live sperm but decreased the percentage of live and motile sperm in both groups. The production of free radicals increased per live cell in both groups, but this increase was significantly higher in the frozen-thawed group than in the fresh group. The results indicated a significant positive relationship between intracellular calcium and the production of oxygen free radicals.   Conclusion: Calcium ions were required for normal sperm function, but increased calcium entry by A23187 into fresh or frozen-thawed sperm causes more damage and reduced motility and survival. The amount of oxygen free radicals had a direct and significant relationship with intracellular calcium. Freezing damage was exacerbated by higher levels of intracellular calcium, which may be due to increased production of oxygen free radicals.    
3066 Biochemistry Evaluation of Gallic Acid Effect on Perphenazine Induced Catatonia in Rats Evaluating the Effect of Gallic Acid on Houshmand GH e Nikbakht J Mahmoudi M g Assadpour S Arab Firozjae A i Arimi AA j Almasian E k e Department of Pharmacology, Mazandaran University of Medical Sciences, Sari, Iran g Department of Pharmacology, Mazandaran University of Medical Sciences, Sari, Iran i Department of Pharmacology, Mazandaran University of Medical Sciences, Sari, Iran j Department of Pharmacology, Mazandaran University of Medical Sciences, Sari, Iran k Department of Pharmacology, Mazandaran University of Medical Sciences, Sari, Iran 1 11 2021 26 5 744 756 21 04 2021 18 10 2021 Background & aim: Catatonia is one of the major signs of Parkinson’s disease which is due to impaired dopamine in extrapyramidal system. This study was performedconducted to elucidate the role of Gallic acid (GA) on perphenazine (PPZ)-induced catatonia in rat.   Methods: This is an experimental study conducted in 2019,48 Wistar male rats weighting 180-220 g were randomly divided into 6 groups (n=8) in each group. Animals were pre-treated with a single dose of normal saline (5ml/kg), most effective dose of bromocriptine (30mg/kg) and GA (100, 200, 400 and 600 mg/kg) via intraperitoneal (IP) route. PPZ (5 mg/kg, IP) was administered after 30 minutes of first injection to induce catatonia. The scoring method of Morpurgo was used to determine the muscular rigidity of animals.   Ethical consideration: This study obtained its ethical approval form the Research Ethics Committee. (Code: IR.MAZUMS.RIB.REC.1398.056). Results: The results indicated that the 100mg/kg GA treated group had no significant reduction in catatonic responses after PPZ administration in comparison with negative control group while the groups that received 400 and 600mg/kg of GA showed significant difference (p<0.05) at all the time points.   Conclusion: The results revealed that GA had protective effect on catatonia induced by PPZ in rats. Hence, GA may probably be supportive for reducing catatonia in Parkinsonism.      3118 Clinical The Comparison of the Effectiveness of Cognitive-Behavioral Hypnosis Therapy and Mindfulness Therapy Based on the Fear of Recurrence in Women with Breast Cancer in Gorgan Yusefi R l Hafezi F m Bakhtiaripour S n Makvandi B o l Department Health Psychology.Khoramshahr International Branch, Islamic Azad University Khoramshahr, Iran m Department of Psychology, Shahid Chamran University, Ahvaz, Ahvaz, Iran n Departmant of Psychology, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran o Departmant of Psychology, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran 1 11 2021 26 5 757 769 18 07 2021 18 09 2021 Background & aim: Cancer is one of the incurable diseases in which in addition to drug and medical treatment, psychological interventions are also effective in reducing its psychological consequences. Therefore, the present study aimed to determine the comparison of the effectiveness of therapy based on cognitive-behavioral hypnosis and mindfulness-based therapy on the dimensions of fear of recurrence in women with breast cancer.   Methods: The present study was a quasi-experimental study using a pretest-posttest method with a control group, which was conducted in 2016-2017. The statistical population of the study was women with breast cancer who referred to medical centers in Gorgan. The sample size was according to the test power (0.80), the effect size (0.50) and the confidence level (0.05) according to the Cohen table for the sample group (15 people). 45 people were selected according to the entry criteria. After selecting the prototype group; It was randomly divided into three groups of 15 and then by random assignment of two groups was considered as an intervention group and the other group as a control group and the research tool was the fear of recurrence questionnaire. The intervention groups underwent group training on cognitive-behavioral hypnosis and group therapy on mindfulness. The collected data were analyzed using analysis of variance, Kolmogorov-Smirnov, Levin and post hoc tests.   Results: The results of analysis of variance indicated that interventions based on cognitive-behavioral hypnosis and mindfulness based therapy had a significant effect on reducing the fear of recurrence. The results of a positive post hoc comparison test correspondingly indicated that cognitive-behavioral hypnosis intervention was more effective in reducing fear of recurrence.   Conclusion: According to the results of the study, the fear of recurrence of the disease in cancer patients under the influence of cognitive-behavioral hypnosis treatment has improved. Consequently, the use of this treatment protocol is recommended for cancer patients.     3086 Psychology Comparison of the Effectiveness of Training Based on Choice Theory and Creativity Training on Resilience and Excitement of Thought in Health Workers in Yasuj Ghanbarnejad AA p Mohammadi K Amirfakhraei A Haji Alizadeh K p Department of General Psychology, Bandar Abbas Branch, Islamic Azad University, Bandar Abbas, Iran, Department of General Psychology, Hormozgan University, Hormozgan, Iran Department of General Psychology, Bandar Abbas Branch, Islamic Azad University, Bandar Abbas, Iran, Department of General Psychology, Bandar Abbas Branch, Islamic Azad University, Bandar Abbas, Iran, 1 11 2021 26 5 770 782 16 05 2021 26 10 2021 Background & aim: The staff of health centers are faced with some stressors such as patients' problems, answering the occasional questions of patients, different work shifts, burnout and work-family conflict. Therefore, the purpose of this study was to determine and compare the effectiveness of training based on the theory of selection and creativity training on the level of resilience and excitement of thought in health workers of Yasuj city.   Methods: The present quasi-experimental study used pre-test-post-test method with a control group conducted from 2018-2019. The statistical population of the study included all health workers in Yasuj who used a sampling method based on Based on the criteria, 45 of these people were randomly selected and divided into two experimental groups and one control group (15 people for each group). Resilience, Thought Excitement Questionnaires were taken from all three groups of subjects, the experimental group received therapeutic creativity training and selection theory training in 8 sessions of 90 minutes for 2 months and the control group did not receive training during this training period. The collected data were analyzed using univariate and multivariate analysis of covariance.   Results: The findings indicated that there was no significant difference between the experimental groups in terms of level dependent variables (F=68.45 and p<0.001). Therefore, the main hypothesis of the research should be rejected, that is, the effectiveness of the two methods of teaching choice theory and teaching creativity on the level of thought excitement, resilience and is the same in health care workers.   Conclusion: The results of the present study revealed that participation in group meetings and increase group actions and reactions and exchange of information and experiences of each employee in training sessions and familiarity with basic needs as well as familiarity of employees with personality components of creative therapy including (confidence, courage and Risk-taking) could lead to increased resilience, dynamism in them.   3173 Clinical Dynamic Screw and Hip Dynamic Screw in Patients Referred to Shahid Beheshti Hospital in Yasuj During 2007-2017 Mohammadi H Department of Orthopedics, Yasuj University of Medical Sciences, Yasuj, Iran 1 11 2021 26 5 783 792 03 10 2021 13 11 2021 Background & aim: Treatment of sub trochanteric fractures is very challenging for bone and joint surgeons. The techniques used to treat these fractures are fracture reduction and the use of condylar dynamic screws and hip dynamic screws. The aim of this study was to compare the treatment of sub trochanteric fractures of type A femur with condylar dynamic screw and hip dynamic screw in hospitalized patients at Shahid Beheshti Hospital, Yasuj, Iran from 2007- 2017.   Methods: The present study was conducted by reviewing the existed data files of 50 patients with sub trochanteric fracture type A hospitalized at Shahid Beheshti Hospital, Yasuj, Iran from 2007- 2017. These patients were randomly divided into two equal groups of 25 in terms of treatment with condylar dynamic screw(DCS) and hip dynamic screw (DHS). In these two groups, the variables of treatment failure, pain, and range of motion of the hip in three-month and six-month periods were compared. The collected data were analyzed using Chi-square and Mann-Whitney tests.   Results: From the 50 patients, 11(22%) failed treatment, of which four (16%) were treated with DHS and seven (28%) with DCS. No statistically significant relationship was seen between age (p=0.1), sex (p=0.3) and type of intervention (p=0.9) with treatment failure. Treatment failure (approximately 90%) occurred in the first four months and all within six months. Both treatments (using DCS and DHS) were optimally effective in the treatment of subtrocentric fractures of type one and no statistically significant difference was observed between them (p <0.001).   Conclusion: Both treatments (using DCS and DHS) were optimally effective in the treatment of sub trochanteric fractures of type A and no statistically significant differences were observed between them.     3083 Microbiology Antibiotic Resistance Patterns of Methicillin and Vancomycin -Resistant Staphylococcus Aureus Isolated from Tehran Hospitals' Clinical Samples Eslamnezhad N Ghandehari F Mirzaee M Madani M Mehrabi MR Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran Department of Biology, School of Basic Sciences, Boroujerd Azad University, Boroujerd, Iran Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran Department of Biology, School of Basic Sciences, Boroujerd Azad University, Boroujerd, Iran 1 11 2021 26 5 793 814 16 05 2021 15 08 2021 Background & aim: One of the most important challenges today is the spread of methicillin and vancomycin resistant strains of Staphylococcus aureus. Acquisition of antibiotic resistance and changes in staphylococcal pathogenicity patterns are the most important causes of virulence. Therefore, the aim of the present study was to determine and identify the pattern of antibiotic resistance in methicillin and vancomycin resistant strains of Staphylococcus aureus isolated from clinical samples of metropolitan hospitals in Tehran.   Methods: In the present cross-sectional descriptive study conducted in 2018, 502 clinical specimens of Staphylococcus aureus were identified and confirmed by biochemical tests and amplification of s_rRNA16 gene by PCR. All isolates were tested for resistance to methicillin by disk method and for resistance to vancomycin using the least inhibitory concentration method. Finally, both groups were tested for molecular weight. The collected data were analyzed using one-way analysis of variance and error coefficient less than 0.05% using statistical tests.   Results: Out of 502 isolates, 168 isolates were resistant to methicillin (33.46%), 6 isolates were resistant to vancomycin (1.19%) and 2 isolates had intermediate resistance to vancomycin (0.39%). The frequency of mecA gene in isolates Methicillin resistance was 98.8%, vanB and vanC1 gene frequency was 37.5% each and vanC3 gene frequency was 12.5% in vancomycin resistant isolates. The highest resistance of methicillin-resistant isolates was isolated from the catheter and the highest resistance of vancomycin-resistant isolates with intermediate resistance to vancomycin were isolated from the secretion site. In both groups, the highest resistance was related to adults.   Conclusion: VRSA and MRSA are considered as a serious threat for human health. Correct detection of the infection resistance patterns and the use of convenient antibiotic can decrease the speed of the development of bacteria resistance.     3199 Nutrition An Analytical Study of Complementary Feeding Pattern Among Healthy and Failure to Thrive Children in Boyer-Ahmad County: A Matched Case-Control Study Pourali S Ghadimi A Khorshodian N Avazpour M Malekzadeh J General Practitioner, Boyer-Ahmad Health Center, Yasuj University of Medical Sciences, Yasuj, Iran, Department of Pediatric, Yasuj University of Medical Sciences, Yasuj, Iran Department of Nutrition, Yasuj University of Medical Sciences, Yasuj, Iran Department of Emergency, Yasuj University of Medical Sciences, Yasuj, Iran Social Determinants of Health Research Center, Yasuj University of Medical Sciences, Yasuj, Iran 1 11 2021 26 5 815 826 07 11 2021 13 11 2021 Background & aim: Children under the age of two are the most vulnerable group of children who are at risk for developmental disorders due to rapid growth and inadequate food intake. Therapeutic measures to solve the nutritional problems of children, especially infants, are often without significant effect and indicate the need for further studies. Therefore, the aim of this study was to determine and compare the complementary feeding pattern of children aged 6-24 months with growth and health disorders in Boyer-Ahmad city.   Methods: In the present analytical case-control study conducted in 2018, 68 underweight children referred to Boyer-Ahmad pediatric and nutrition clinics, or selected from the list of malnourished children in health centers were compared to 61 healthy control children in terms of nutritional patterns and socio-economic status. The variables of parents' education, parents' occupation, mothers' knowledge, attitude and practices toward child nutrition and their 24-hour food intake were evaluated. The data were analyzed using Chi-square, independent samples t-test and Man-Whitney tests.   Results: There was no statistically significant difference between the two groups of children in terms of energy, protein, fat, iron, zinc and vitamin A intake, but the amount of carbohydrate intake in children with FTT showed a statistically significant difference with healthy control children (p = 0.003). The most important factors related to FTT in children were the nutritional knowledge of the mothers (p = .001), mother's education (p = .02), father's education (p = 0.033), frequency of complementary food consumption (p = .017) and time Consumption of snacks (p = .017) by children. The variables of parental employment status, age of starting complementary feeding, type of milk consumed were not statistically different between the children with FTT and controls.   Conclusion: Nutritional information of mothers, frequency of complementary feeding, and food preparation hygiene, time of feeding snacks and education level of parents had the most relationship with growth of children.         2914 Occupational Health Evaluate the Ergonomic Status of Workstations in Gachsaran Health Center in 2019 Pakzad A Ghavam A Aghaei Borzabad P Department of Health Services Management, Marvdasht University, Marvdasht, Iran 2Institute of Advanced Science and Technology and Environmental Sciences, Graduate University of Industrial and Advanced Technology, Kerman, Iran Center for Factors Affecting Health, Yasuj University of Medical Sciences, Yasuj, Iran 1 11 2021 26 5 827 837 14 09 2020 31 10 2021 Background & aim:  The human living environment should be designed in accordance with his physical, mental and physical condition, so that no pressure or harm is inflicted on him. Also, if you give them any, all, or all of them, they will cause musculoskeletal disorders. Therefore, the purpose of the present study was to determine and evaluate the ergonomic status of workstations in Gachsaran health center.   Methods: The present descriptive qualitative study was conducted in 2019. The study population included the staff of Gachsaran health center from which 10 people were selected. A non-random purposive method was used to select participants in the study. This continued until data saturation, the data collection tool being a semi-structured interview. In order to analyze the data, the latent content analysis method was used.   Results: The findings led to the extraction of the main themes of ergonomic health center status and 5 main themes; workstations comprising 16 proprietary codes, ergonomic knowledge comprising 7 proprietary codes, musculoskeletal disorders comprising 18 proprietary codes, organizational performance comprising 13 proprietary codes, and realization barriers comprising 8 codes. Became dedicated. Ergonomic knowledge includes the awareness, attitude and behavior of individuals. Orumi implementation is implemented in a system when all users of the system with the necessary ergonomic knowledge can be implemented in the principles of an organization, users of that system suffer from work-related injuries and diseases, are satisfied with the conditions of the workplace. One of the factors that can be understood in the principles of ergonomics in the workplace.   Conclusion: Workstations, ergonomic knowledge, musculoskeletal disorders, organizational performance and barriers to the realization of five themes were identified in the present study. It is suggested that managers change their attitude toward ergonomics while developing a workstation evaluation program to provide effective interventions to improve workstations. As well as practical training in providing employees with ergonomic principles.     3057 Clinical Evaluation of the Effects of Phototherapy on Neonatal Serum Magnesium Levels: A Systematic Review Nabi Meybodi M Parsai H Abdollahi Fard GH Mousavi SSH Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran Department of Physics and Medical Engineering, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Departments of Social Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran 1 11 2021 26 5 838 850 08 04 2021 01 11 2021 Background & aim: Neonatal jaundice with a prevalence of 60% in the first weeks of life is one of the most common neonatal diseases that occurs due to elevated serum bilirubin levels. Phototherapy is the most common method for the treatment of neonatal jaundice, since the effect of phototherapy on serum magnesium levels in infants is not well understood, as a result, the aim of the present study was to determine the effect of phototherapy on serum magnesium levels in neonatal hyperbilirubinemia.   Methods: In the present systematic review article in 2021, domestic databases of University Jihad, Civilica, and university journals in addition to PubMed database were searched without time limit. Studies which assessed the effect of phototherapy on serum magnesium level were reviewed and the results were reported. All articles were separately reviewed by two authors. The data were interpreted through comparative analysis.   Results: Magnesium serum level was measured in less than 24 hours after phototherapy in eight studies, 48 hours in four, and 72 hours in three. Two hundred and twenty-two articles were extracted from the mentioned databases. Applying the inclusion and exclusion criteria, 14 articles were selected for thorough assessment. As shown in most of these studies, phototherapy had a significant effect on decreasing serum magnesium levels in neonates. Only one study did not show any significant effect. Most studies have not evaluated demographic and clinical variables in decreasing serum magnesium levels.   Conclusion: There was a significant relationship between phototherapy and serum magnesium levels. Considering the role of NMDA receptor and its stimulatory effect in brain damage due to hypoxia and hyperbilirubinemia and the importance of magnesium in its inhibition, the body increases the extracellular magnesium levels during neonatal jaundice to prevent brain damage. It was concluded that after phototherapy, and therefore resolution of hyperbilirubinemia, serum magnesium level was reduced.     3156 Health Management Investigating the Components of Research Performance of Faculty Members of Yasuj University of Medical Sciences Hosseini M Ahmadi E Shahamat N Amirianzadeh M Department of Management, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran Department of Management, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran Department of Management, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran Department of Management, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran 1 11 2021 26 5 851 866 03 09 2021 10 11 2021 Background & aim: Giving importance to research and increasing research activities in each country provides appropriate contexts for the development and progress of that country. The present study was conducted to identify components related to the performance of faculty members of Yasuj University of Medical Sciences.   Methods: The present research was a qualitative, exploratory study conducted in year of 2021. In present study, purposeful sampling was used. Data collection was performed through semi-structured in-depth interviews, face to face and telephone calls. The key participants in the study were faculty members of Yasuj University of Medical Sciences, of whom 21 were selected by purposeful sampling and interviewed. Each interview lasted an average of 40 to 60 minutes. Atrid-Sterling method was also used for analysis.   Results: Twenty faculty members of Yasuj University of Medical Sciences participated in the present study, 9 females (45 percent) and 11 males (55 percent). In terms of academic rank, four were full professors, 7 were associate professors, and 10 were assistant professors. Moreover, after analyzing the interview data and first coding, 80 basic themes were obtained, which were then reduced to fourteen organizing themes. To end with, global themes policy, individual and situational were identified as factors related to faculty research performance.   Conclusion: To achieve a comprehensive performance in the field of research depends on proper and correct planning in this area. Situations such as team thinking, knowledge-based, and community-oriented can improve faculty members' research performance.