TY - JOUR T1 - Effect of High Intensity Endurance Training on p53 and Cytochrome-c Gene Expression in Male Rat Soleus Muscle TT - اثر تمرین استقامتی با شدت بالا بر بیان ژن‌های p53 و سیتوکرومc عضله‌ نعلی موش‌های صحرایی نر JF - yums-armaghan JO - yums-armaghan VL - 22 IS - 5 UR - http://armaghanj.yums.ac.ir/article-1-1571-en.html Y1 - 2017 SP - 608 EP - 622 KW - High intensity endurance training KW - p53 gene KW - Cytochrome-c gene KW - Soleus muscle N2 - Abstract Background and aim: Recent research has suggested that exercise training is potential to influence the process of programmed cell death. However, little is known about the effect of high intensity endurance exercise on apoptotic factors. Therefore, in the present study the effect of high intensity endurance training on p53 and cytochrome-c gene expression in male rats' Soleus muscle was investigated. Methods: In this experimental study sixteen 3-month-old male rats were randomly divided into two groups of endurance training (n=8) and control (n=8). Exercise program included three months endurance training at the intensity of 75-80% of VO2max with the frequency of 5 days per week. Forty-eight hours following the last training session, the soleus muscle of the animals were extracted and p53 and cytochrome-c gene expression were assessed using Real Time-PCR method. Data were analyzed by independent t-test at the significance level of P<0.05. Results: No significant difference was seen between the experimental and control groups regarding p53 gene expression (P=0.67). However, cytochrome-c gene expression was significantly lower in the experimental group than the control group (P=0.001). Conclusion: High intensity endurance training decreased cytochrome-c gene expression which is likely mediated by improved mitochondrial membrane stability and decreased permeability. It may influence the process of apoptosis in soleus muscle. However, it needs to be examined and validated in future studies. M3 ER -