%0 Journal Article %A Ahmadi, A %A Jamshidi, F %T Evaluation of the Protective Effect of Satureja khozestanica Essential Oil on the Potential Fertilization in Vitro and the Process of Embryonic Growth in Syrian Mice Under the Oxidative Stress Induced by Nonophenol %J Armaghane Danesh %V 25 %N 2 %U http://armaghanj.yums.ac.ir/article-1-2431-en.html %R 10.52547/armaghanj.25.2.201 %D 2020 %K In Vitro Fertilization, Nonyl Phenol, Oocyte, Satureja khuzestanica, %X Background & aim: Nano phenol (NP) is known to be an environmental contaminant and an immunosuppressant agent, which has adverse effects on sex cell’s nucleus, inducing stress, oxidative stress and reducing fertility. The aim of the present study was to determine the protective role of Satureja khozestanica as an antioxidant on the growth process of fetuses resulting from in-vitro fertilization following Nano phenol administration. Methods: In the present experimental study conducted in 2016, 40 mature female mice, aged 6-8 weeks, weighing 20-25 grams, were randomly divided into 4 equal groups. The control group received corn oil orally and the test groups were treated with NP (225mg/kg), khozestanica essential essence (225 mg/kg), NP(225mg/kg) + khozestanica essential essence (225mg/kg) for 28 consecutive days orally by gavages. PMSG and HCG were administrated for stimulating the superovulation process. The sperms were obtained from 6 mature male mice. Following oocyte collection in-vitro fertilizing was performed using HTF+4mgBSA medium. The fertilized oocytes were incubated for 120 hours and the embryo development were studied. Two proportion test was used for statistical analyses. (p<0.05). Results: A significant decrease in all quality parameters of oocyte and embryo, fertilization percentage (65.96), percentage of two-celled embryos (64.52) and blastocyst (29.03) were observed in the Nano Phenol group in comparison with the control group. Prescribing khozestanica essential essence was able to prevent the adverse effects of nanoparticles on the maturity of oocytes and cause a significant increase in the quality and number of oocytes. Due to the antioxidant and androgenic effects of khozestanica essential essence, its administration together with Nano phenol was able to prevent reproductive toxicity and significantly increase the fertilization rate (95.83), do cellulite (86/96), blast cyst (6/57). Conclusion: Co-administration of the khozestanica essential essence with Nanophenol reduced toxic effects on the number and oocyte’s quality, in-vitro fertilization potential, improved the embryo development, and reduced the arrested embryo. %> http://armaghanj.yums.ac.ir/article-1-2431-en.pdf %P 201-212 %& 201 %! %9 Research %L A-10-1743-1 %+ Department of Basic Sciences, Urmia University, Urmia, Iran %G eng %@ 1728-6506 %[ 2020